Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of citicoline

A technology of citicoline and phosphorylcholine, which is applied in the field of preparation of citicoline, can solve the problems of increased cost of citicoline, expensive substrates, cumbersome process steps, etc., and achieves easy reaction conditions Effect of control, low production cost and low substrate cost

Inactive Publication Date: 2011-09-28
苏州天马医药集团天吉生物制药有限公司
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current citicoline production methods require the participation of a variety of microorganisms, making the production process conditions relatively harsh and the process steps relatively cumbersome; and the current citicoline production methods use substrates Most of the prices are relatively expensive, which greatly increases the cost of producing citicoline

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of citicoline
  • Preparation method of citicoline
  • Preparation method of citicoline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The preparation method of citicoline of the present invention uses the culture of a single genetically engineered bacterium or its processed product as an enzyme source to catalyze the reaction of substrates including ammonium chloride, orotic acid and phosphorylcholine, so that the reaction solution generate and accumulate citicoline in the reaction liquid, and extract the citicoline from the reaction solution.

[0024] 【Example】

[0025] The genetically engineered bacteria used in this example is a single strain, which can simultaneously induce the expression of yeast phosphorylcholine transcytidylase (CCT) and orotidine pyrophosphorylase (pyrE) in the pyrimidine metabolic pathway of Escherichia coli , orotidine decarboxylase (pyrF), uridylate kinase (pyrH), nucleoside diphosphate kinase (NDK) and cytidine triphosphate ligase (pyrG).

[0026] Primers were designed according to the known cct gene sequence of Saccharomyces cerevisiae (genebank accession number: M36827)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of citicoline. The method comprises the following steps: the culture of a single gene engineering bacterium or the treatment material of the culture is used as enzyme source to catalyze the reaction of a substrate comprising ammonium chloride, orotic acid and phosphocholine and ensure that citicoline is generated and accumulated in the reaction solution, and citicoline is extracted from the reaction solution. The invention has the following beneficial effects: (1) the single microorganism is utilized to catalyze the reaction, reaction conditions are easy to control; (2) the cost of the substrate is lower so that the production cost of citicoline is lower; and (3) the reaction is fast and the conversion rate is higher. The method can be widely used in the preparation of citicoline.

Description

technical field [0001] The invention relates to a preparation method of citicoline, which belongs to the field of biotechnology. Background technique [0002] Citicoline, also known as cytidine diphosphate choline, was named citicoline in the Chinese Pharmacopoeia 2005 edition, English name: Citichaline, and its chemical name: choline cytosine nucleoside-5'-diphosphate. It is a precursor for the biosynthesis of lecithin, and when brain function declines, the lecithin content in brain tissue is significantly reduced. Supplementing exogenous citicoline can activate the biosynthesis of lecithin, stimulate the excitement of the brainstem reticular structure, increase the threshold of awakening, restore the function of nerve tissue, improve brain metabolism and nerve conduction, and improve the patient's consciousness level. [0003] In the 1950s, Dr. Kennedy and others discovered and determined the molecular structure, and later Rossiter and others clarified its function. In t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/30C12N15/70C12R1/19
CPCY02P20/582
Inventor 汪仁谢建新李晓丹王进全夏冰
Owner 苏州天马医药集团天吉生物制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com