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Extraction method of teasel root total saponins

An extraction method and technology of total saponins are applied in the field of extraction of discontinuous total saponins, which can solve the problems of polluted environment, cumbersome process and high production cost, and achieve the effects of reducing production energy consumption, simple extraction process and reduced use.

Active Publication Date: 2013-05-01
浙江迪耳药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting total saponins of Dipsacus, which can overcome defects such as the use of a large amount of organic solvents in the existing extraction method, complicated process, high production cost, and environmental pollution. It is a simple process and easy industrial production. A method for extracting total saponins of Radix Dipsacus that can significantly reduce production costs and be more environmentally friendly

Method used

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  • Extraction method of teasel root total saponins
  • Extraction method of teasel root total saponins
  • Extraction method of teasel root total saponins

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Take the Dipsacus medicinal material, dry it, and crush it into a coarse powder of 10-80 mesh. Take 80kg of Dipsacus powder, add 200L, 150L, ​​and 150L of 90% ethanol, respectively, and reflux and extract three times at 80°C for 2, 2, and 0.5 hours respectively. Combine the extracts, concentrate and recover the ethanol until it has no alcohol smell , add 20L water and heat at 80°C to dissolve, filter to obtain the filtrate; add 40L petroleum ether (boiling range 60-90°C) to the filtrate and stir evenly, keep stirring at 35°C for 30 minutes for deesterification, let stand for 4 hours, release the lower aqueous solution after layering , crystallization, filtering to obtain the crude product of Dipsacus total saponins, adding 32 kg of ethanol with 20% concentration to the crude product, heating and dissolving, recovering ethanol, drying, and obtaining 8.0 kg of total saponins.

[0022] In the obtained extract, the weight percent content of total saponins of Dipsacus is 84....

Embodiment 2

[0024] Take the Dipsacus medicinal material, dry it, and crush it into a coarse powder of 30-60 mesh. Take 80kg of Dipsacus powder, add 200L, 150L, ​​and 150L of absolute ethanol, reflux and extract three times at 78°C for 3, 2, and 1 hours respectively, combine the extracts, concentrate and recover the ethanol until there is no alcohol smell, add 40L Heat water at 70°C to dissolve, filter to obtain the filtrate; add 80L of petroleum ether (boiling range 60-90°C) to the filtrate and stir evenly, keep stirring at 45°C for 45 minutes to deesterify, let stand for 8 hours, release the lower aqueous solution after layering, and crystallize , filtered to obtain the crude product of Dipsacus total saponins, the crude product was added with 32 kg of ethanol with a concentration of 35% and heated for dissolving to recover ethanol, and dried to obtain 8.0 kg of total saponins.

[0025] In the obtained extract, the weight percent content of Dipsacus saponins is 92.9%, and Dipsacus saponi...

Embodiment 3

[0027] Take the Dipsacus medicinal material, dry it, and crush it into a coarse powder of 30-80 mesh. Dipsacus coarse powder 80kg, add 91% concentration of ethanol 300L, 200L, 150L, ​​reflux extraction at 80 ℃ for three times, the extraction time is 3, 2, 2 hours respectively, combine the extracts, concentrate and recover ethanol until there is no alcohol smell, add Heat 80L of water at 55°C to dissolve, filter to obtain the filtrate; add 160L of petroleum ether (boiling range 60-90°C) to the filtrate and stir evenly, keep stirring at 55°C for 45 minutes for deesterification, let stand for 10 hours, release the lower layer of aqueous solution after separation, and analyze crystal, filtered to obtain the crude product of Dipsacus total saponins, the crude product was added with 30 kg of ethanol with 50% concentration, heated and dissolved, and then the ethanol was recovered, dried to obtain 11.2 kg of total saponins.

[0028] In the obtained extract, the weight percent content ...

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Abstract

The invention discloses an extraction method of teasel root total saponins, which comprises the following steps: pulverizing teasel roots into coarse powder, adding ethanol, performing heating reflux for several times, or performing heating reflux for 1 hour, then performing circulating reflux till the extract is approximately colorless, combining the extract, recovering ethanol till no ethanol smell exists, adding a proper amount of water, heating for dissolution, and filtering; extracting and degreasing the filtrate by petroleum ether, allowing the filtrate to stand so as to separate the water phase, precipitating the precipitate, filtering to obtain a teasel root total saponin crude product, adding ethanol into the crude product, heating and dissolving the product, recovering ethanol, and drying to obtain a teasel root total saponin extract. The method of the invention has a simple extraction process, does not adopt n-butanol extraction during the extraction process, omits repeatedextraction steps, simplifies the extraction process, reduces the production cost, decreases the use of toxic organic solvents (n-butanol), and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from Dipsacae, in particular to a method for extracting total saponins of Dipsacae. Background technique [0002] Dipsacus is the dry root of Dipsacus asperoides.Y.Cheng et T.M.Ai, a plant of Dipsacaceae. It has the functions of nourishing the liver and kidney, connecting bones and muscles, resisting osteoporosis, stopping bleeding and preventing miscarriage. It is mainly used clinically. It is used to treat soreness of the waist and knees, bruises, fractures, lumbar hyperosteogeny, threatened abortion and habitual abortion, etc. Its main active ingredient is triterpene saponin. Modern studies have shown that the main chemical components in Dipsacus are: saponins, iridoids, alkaloids, volatile oils, sucrose, sterols, calcium, iron, magnesium, manganese, zinc, copper and other trace elements and a small amount of aglycones. At present, more than 20 kinds of saponins have been isolated ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P15/06A61P19/08
Inventor 张龙清胡献跃金灵华方肖庆黄秀清
Owner 浙江迪耳药业有限公司
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