Sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and preparation method thereof

A nanocomposite and semi-interpenetrating technology, applied in the field of sericin-based semi-interpenetrating temperature-sensitive nanocomposite hydrogel and its preparation, can solve problems such as insufficient biocompatibility, achieve excellent cell compatibility, and prepare methods Simple, low-cost preparation effects

Inactive Publication Date: 2011-10-05
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to design a sericin-based semi-interpenetrating temperature-sensitive nanocomposite hydrogel, so that the gel has high transpar...

Method used

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  • Sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and preparation method thereof
  • Sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] 1) Dispersion of inorganic nano-saponite particles: at 20°C, add hectorite to deionized water to prepare an aqueous solution with a concentration of 1% by mass, and stir for 25 minutes until completely dispersed to form a transparent solution;

[0036] 2) Preparation of the reaction pre-polymerization liquid: under the protection of nitrogen, add sericin and isopropylacrylamide to the above-mentioned transparent solution in sequence, and the concentration of sericin and alkylacrylamide monomers in the aqueous solution is 1% by mass respectively and 9%, stirred at 20°C for 25 minutes to form a slightly pale yellow reaction prepolymer solution;

[0037] 3) Preparation of gel: Cool the above reaction prepolymerization solution to 2°C with ice cubes, add an aqueous initiator solution with a mass percentage concentration of 0.1% under nitrogen protection and magnetic stirring, stir for 15 minutes, and inject a mass percentage concentration of 0.1% % catalyst aqueous solution...

Embodiment 2

[0041] 1) Dispersion of inorganic nano-saponite particles: at 22°C, lithium magnesium silicate was added to deionized water to prepare an aqueous solution with a concentration of 1.5% by mass, and stirred for 25 minutes until completely dispersed to form a transparent solution;

[0042] 2) Preparation of the reaction pre-polymerization solution: under the protection of nitrogen, add sericin and isopropylacrylamide to the above-mentioned transparent solution in sequence, and the mass percent concentration of sericin and alkylacrylamide monomers in the aqueous solution is 2% respectively and 8%, stirred at 22°C for 30 minutes to form a slightly pale yellow reaction prepolymer solution;

[0043] 3) Preparation of gel: Cool the above reaction prepolymerization solution to 1° C. with ice cubes, add an aqueous initiator solution with a mass percentage concentration of 0.15% under nitrogen protection and magnetic stirring, stir for 20 minutes, and inject a mass percentage concentratio...

Embodiment 3

[0047] 1) Dispersion of inorganic nano-saponite particles: at 25°C, add hectorite to deionized water to prepare an aqueous solution with a concentration of 3% by mass, and stir for 30 minutes until completely dispersed to form a transparent solution;

[0048] 2) Preparation of the reaction pre-polymerization solution: under the protection of nitrogen, add sericin and isopropylacrylamide to the above-mentioned transparent solution in sequence, and the mass percent concentrations of sericin and alkylacrylamide monomers in the aqueous solution are 3% respectively and 7%, stirred at 25°C for 35 minutes to form a slightly pale yellow reaction prepolymer solution;

[0049] 3) Preparation of the gel: Cool the above reaction pre-polymerization liquid to 1°C with ice cubes, add an aqueous initiator solution with a mass percentage concentration of 0.1% under nitrogen protection and magnetic stirring, stir for 15 minutes, and inject a mass percentage concentration of 0.2 % catalyst aqueo...

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Abstract

The invention relates to a sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel and a preparation method thereof. The hydro-gel comprises a sericin polypeptide chain part, an inorganic nano-soapstone particle part and a polymer formed after an alkylacrylamide monomer is polymerized. In the hydro-gel, a semi-interpenetrating network technology is mainly adopted, and by combining the physical crosslinking effect of inorganic nano-soapstone particles, the sericin, the inorganic nano-soapstone particles and in-situ free radical of the alkylacrylamide monomer are polymerized. The preparation method comprises the following steps of: dispersing the inorganic nano-soapstone particles; preparing a reaction prepolymerization solution; preparing gel; and purifying the gel. The sericin-based semi-interpenetrating temperature-sensitive nano-composite hydro-gel has rich raw material sources, simple preparation method, low cost, high swelling degree and excellent cell compatibility, can be adjusted according to the content of sericin, and has a wide application prospect in artificial skin, artificial cartilage, cell culture stent, bioreactor and other biomedical aspects.

Description

technical field [0001] The invention relates to the fields of biological materials and intelligent polymer materials, in particular to a sericin-based semi-interpenetrating temperature-sensitive nano-composite hydrogel and a preparation method thereof, which can be applied to technical fields such as biomedicine, tissue engineering, and drug release. Background technique [0002] Silk is composed of silk fibroin and sericin. Sericin wraps around the silk fibroin and accounts for about 25% of the mass of the cocoon layer. It is mainly used to glue and protect the silk fibroin. In addition, it also contains a small amount of wax, pigment, and carbohydrates. and inorganic components. Sericin (SS) is a globular protein with a relative molecular mass of 14,000 to 314,000 and composed of 18 amino acids. Polar side chain amino acids account for 74.61% of the amino acid composition of sericin, so sericin has good water absorption, water solubility, anti-oxidation, promotion of cell...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L33/24C08L89/00C08K3/34C08F120/54C08F2/44
Inventor 张青松李学伟陈莉郭冠群赵义平
Owner TIANJIN POLYTECHNIC UNIV
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