Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for extracting alpha-amylase inhibitor from Fuzhuan tea

An amylase inhibitor and Fuzhuan tea technology, which can be used in medical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the research reports on the extraction of active substances of α-amylase inhibitors in fuzhuan tea that have not yet been seen. problem, to achieve good inhibitory activity, increase added value, and simple preparation process

Inactive Publication Date: 2011-10-12
NANTONG GUOMIQI MASCH EQUIP CO LTD
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers at home and abroad have studied the biological activity of Fuzhuan tea from different angles, discussed the effects of Fuzhuan tea on weight loss, lowering blood fat, anti-tumor, and treating diarrhea, and achieved good results. Research Report on the Extraction of α-amylase Inhibitor Active Substances in Brick Tea

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting alpha-amylase inhibitor from Fuzhuan tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Determination of α-amylase inhibitor activity

[0017] Utilize 3,5-dinitrosalicylic acid method (DNS method) to measure, specifically as follows: 0.2mL α-amylase solution (prepared with pH 6.9, 20mmol / L phosphate buffer containing 6.7mmol / L NaCl) and 0.2 mL of extracts containing different concentrations of α-amylase inhibitors were mixed evenly, and placed in a 37°C water bath to preheat for 15 minutes, then 0.6mL of 10g / L starch solution preheated to 37°C was added. After reacting for 5 minutes, 1.0 mL of DNS color reagent was added immediately. Develop color in boiling water for 5 minutes, dilute with 8 mL of deionized water after cooling in an ice-water bath, take 0.2 mL of the sample into a 96-well plate, and measure the absorbance at 540 nm with a microplate reader. The sample without α-amylase inhibitor was used as the control. The calculation method of the inhibition rate of α-amylase inhibitor to α-amylase is:

[0018] Inhibition rate (%)=(1-α-amy...

Embodiment 2

[0019] Embodiment 2: Determination of the alcohol extraction process with reference to the inhibition rate of α-amylase inhibitors to α-amylase activity:

[0020] According to the design principle of the Box-Behnken central combination experiment and the results of the single factor experiment, three factors, ethanol concentration, extraction time and extraction temperature, which have a significant impact on the extraction rate of inhibitors were selected, and three factors and three levels were adopted on the basis of the single factor experiment. response surface analysis method. The specific implementation method is: take 3.00 g of special Fuzhuan brick tea (purchased from Hunan Yiyang Tea Factory, the same below) powder that is crushed to 20 mesh, add 30 mL of ethanol aqueous solution with a concentration of 40% to 60% (v / v), and extract for 1h to 2h , the extraction temperature is 40°C to 60°C, and the extraction is performed twice under the same conditions. The extract...

Embodiment 3

[0024] Embodiment 3: the preparation of α-amylase inhibitor

[0025] (1) Take 10.0 g of Fuzhuan brick tea powder crushed to 40 mesh, add 100 mL of ethanol with a concentration of 55% (v / v), extract at a constant temperature of 54 ° C for 105 min, extract twice under the same conditions, combine the extracts, and concentrate at low temperature and reduced pressure to 70 mL ;

[0026] (2) The concentrated solution is extracted 4 times with an equal volume of chloroform, and the aqueous phase solution is extracted 4 times with an equal volume of ethyl acetate, and the extraction part of the ethyl acetate is separated, and dried in a low-temperature vacuum with a rotary evaporator to obtain α-amylase inhibition 0.71g, the half-inhibitory concentration of α-amylase (IC 50 ) was 1.09 mg / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for extracting an alpha-amylase inhibitor from Fuzhuan tea. The method comprises the following steps of: (1) crushing and sieving Fuzhuan tea, adding the obtained tea powder into an ethanol water solution of which the weight is 8-15 times of the weight of the tea powder and the concentration is 40-60%, extracting for 1-3 times at the constant temperature of 40-60 DEG C, mixing the extracting solutions, and concentrating to obtain a concentrated solution; and (2) extracting the concentrated solution obtained in the step (1) for 3-5 times with chloroform, separating and taking a water phase solution, extracting for 3-5 times with ethyl acetate, separating and taking an ethyl acetate phase solution, and drying under the reduced pressure to obtain the alpha-amylase inhibitor. The invention mainly has the beneficial effects that: by using the Fuzhuan tea as a raw material for preparing the alpha-amylase inhibitor, the preparation processes are simple, and the obtained product has better inhibition activity to alpha-amylase and is favorable for improving the added value of the Fuzhuan tea. The invention provides a method for further developing the Fuzhuantea into health foods and functional foods.

Description

(1) Technical field [0001] The invention relates to a method for extracting α-amylase inhibitor from Fuzhuan tea. (2) Background technology [0002] α-amylase inhibitor is a new type of drug for the treatment of diabetes and obesity. It can reduce the digestion and absorption of carbohydrates in food by inhibiting the biological activity of the key enzyme α-amylase in human glucose metabolism, thereby achieving hypoglycemia and weight loss. role. Compared with other hypoglycemic and weight loss products, the side effects are relatively small, so the development of α-amylase inhibitors has a good application prospect for the hypoglycemic and weight loss of specific groups of people. [0003] Fuzhuan brick tea is a kind of dark tea made from black hair tea, which is processed through screening, steaming, retting, pressing, flowering and drying. It is divided into special Fuzhuan tea and ordinary Fuzhuan tea. The main difference lies in the blending of raw materials: all the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P3/10
Inventor 王普姜运耀何军邀
Owner NANTONG GUOMIQI MASCH EQUIP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com