Culture medium and culture method of Fuscoporia punctata first level strain

A technology of layered pore bacteria and cultivation methods, applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of high cost, unfavorable large-scale industrial production, complex and cumbersome manufacturing process, strong labor force, etc., and achieve low cost and energy consumption. The effect of small, less labor force

Active Publication Date: 2011-10-19
三明市三真药业有限公司
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Problems solved by technology

It also solves the technical problems of complex and cumbersome production process of the existing layer of pore bacteria

Method used

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  • Culture medium and culture method of Fuscoporia punctata first level strain
  • Culture medium and culture method of Fuscoporia punctata first level strain
  • Culture medium and culture method of Fuscoporia punctata first level strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The strains of Laminaria spp. were provided by Sanming Sanzhen Biotechnology Co., Ltd., and the mother species slant: PDA medium.

[0023] The culture medium of the first-grade strain of Poreus laminatus is: 3% corn flour, 2% brown sugar, 0.3% peptone, 0.01% VB6, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, and 0.05% calcium chloride.

[0024] Preparation method of culture medium: Weigh each ingredient according to the formula, add water and stir evenly, put into 150ml / 500ml Erlenmeyer flask, pH5.0-6.0, 0.1Mpa sterilization for 30 minutes.

[0025] The culture conditions of the first-grade strains of the submerged submerged bacteria: the temperature of the shaker flask is controlled at 27-31°C, the filling volume is 150ml / 500ml, the optimum pH is 5.5-6.0, and the rotation speed is 180-220 rpm. Culture time About 80 hours to 120 hours. Obtain a layered recumbent pore with many and dense bacterial balls, an average bacterial count of 280-300 / ml, a bacteri...

Embodiment 2

[0029] 1. The stratum decumbens strains are provided by Sanming Sanzhen Biotechnology Co., Ltd., the mother species slope: PDA medium. Cultivation method: 250ml triangular Erlenmeyer flask of the first grade shaker, with a liquid volume of 150ml, inoculate a piece of 0.5cm strain. Set up a rotary shaker with a rotating speed of 180 rpm and a temperature of 27-31°C. Each test was repeated three times, and the results were averaged.

[0030] Biomass: After sample preparation and fermentation, take 100ml of the culture solution and subject it to 2000 rpm, centrifuge for 30 minutes, remove the supernatant, take the mycelia and weigh the wet weight of the mycelia to determine the biomass.

[0031] 2. The effect of medium composition on the yield of liquid fermentation mycelium.

[0032] 2.1 Carbon source selection test: the starch carbon source in the basal medium (the basal medium contains the basic nutrients needed for fungal growth and reproduction, which can be used for the gr...

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Abstract

The invention provides a culture medium formula and a submerged culture method of a Fuscoporia punctata first level strain. The culture medium of Fuscoporia punctata adopts brown sugar and corn flour as a carbon source of a fermentation process, peptone as a nitrogen source of the fermentation process, and a vitamin VB 6 as a fermentation nutrient. A Fuscoporia punctata first level strain obtained through the culture medium of Fuscoporia punctata has the characteristics of the number of bacterium balls of 280 to 300 per milliliter, bacterium ball diameter of 0.5 to 1.5 millimeters, uniform bacterium ball size, clear culture liquid, plenty of the bacterium balls, fine bacterium ball size, compact bacterium ball arrangement, and plenty of mycelia germination points thus is beneficial for a secondary fermentation process. The culture method of a Fuscoporia punctata liquid culture medium has the advantages of simple technology and low energy consumption, thus is applicable to large-scale industrial production.

Description

technical field [0001] The invention relates to a culture medium and a culture method for fungal culture, in particular to a culture medium and a culture method for the first-grade submerged fermentation strains of the fungi. Background technique [0002] Porina laminarius, also known as Inonotus zebras, belongs to the genus Aphylloides, Polyporaceae, and the genus Pleuronotus. The shape is perennial fruiting body, sterile cap, flat and flat than the surface of the substrate, up to 20 cm or larger in width, cracked when dry, multi-layered fungal tubes, each layer is 2-3 mm thick, and shrinks year by year, forming Oblate hemispherical fruiting body with a total thickness of 15mm. The surface of the tube hole is rusty brown at the beginning, and later becomes light smoke to brownish gray due to being filled with gray hyphae. The edge of the fruiting body is gray-black, and the wall of the tube hole is thick and complete. The orifice is round, 6-8 per mm. Spores spherical or ...

Claims

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Application Information

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IPC IPC(8): C05G1/00
Inventor 郑秀莲
Owner 三明市三真药业有限公司
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