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Human epidermal growth factor nanoliposome and its preparation method

A technology of epidermal growth factor and nano-liposome, which is applied in skin care preparations, skin diseases, pharmaceutical formulations, etc., can solve the problems of unstable and long-acting release, hEGF cannot be quickly and completely absorbed by tissues, and inactivated. Achieve good epidermal penetration, improve transdermal absorption, and improve stability

Inactive Publication Date: 2011-11-23
TIANJIN TIANSHI BIOLOGICAL DEV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] In order to solve the problems existing in the prior art, the present invention provides a human epidermal growth factor nanoliposome and a preparation method thereof, which solve the problem that in the prior art, direct contact between hEGF and the external environment leads to inactivation, and hEGF cannot be rapidly and completely absorbed. Tissue absorption, release unstable, long-acting problems

Method used

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  • Human epidermal growth factor nanoliposome and its preparation method
  • Human epidermal growth factor nanoliposome and its preparation method
  • Human epidermal growth factor nanoliposome and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0066] According to the prescription volume 1ml unit, the prescription is as follows:

[0067] Prescription one:

[0068] Human Epidermal Growth Factor 1mg

[0069] Lecithin 182mg

[0070] Cholesterol (pharmaceutical grade) 18mg

[0071] Chloroform (analytical grade) 5ml

[0072] Phosphate buffer 1ml

[0073] Accurately weigh 182mg of lecithin, 18mg of cholesterol (PC: CHOL = 10: 1) dissolved in 10ml of chloroform ultrasonic (time 5min, frequency 53kHz) dissolved, after thorough mixing, add 1ml of hEGF aqueous solution (1mg / ml, drug substance ratio = 1: 100), ultrasonically mixed for about 4 minutes to form a W / O emulsion, decompressed (vacuum degree 800mbar, temperature 40°C) to remove the organic solvent to form a colloidal substance, then added 1ml of phosphate buffer, and ultrasonicated for 10min to obtain 1ml milky white uniform hEGF nano liposomes.

[0074] Preparation sample appearance: milky white homogeneous liquid

[0075] Encapsulation efficiency result: 30.8...

Embodiment 2

[0080] Prescription two:

[0081] According to the prescription volume 1ml unit, the prescription is as follows:

[0082] Human Epidermal Growth Factor 1mg

[0083] Lecithin 80mg

[0084] Cholesterol (pharmaceutical grade) 20mg

[0085] Chloroform (analytical grade) 5ml

[0086] Phosphate buffer 1ml

[0087] Preparation process parameters:

[0088] Accurately weigh 80mg of lecithin, 20mg of cholesterol (PC: CHOL = 4: 1) and dissolve in 5ml of chloroform by ultrasonic (time 2min, frequency 35kHz) to dissolve, after mixing thoroughly, add 1ml hEGF aqueous solution (1mg / ml, ratio of medicine to substance = 1: 100), ultrasonically mixed for about 4min to form a W / O emulsion, decompressed (vacuum degree 750mbar, temperature 35°C) to remove the organic solvent to form a gel-like substance, then added 1ml of phosphate buffer, and ultrasonically for 10min to obtain 1ml milky white uniform hEGF nano liposomes.

[0089] Preparation sample appearance: milky white homogeneous liqui...

Embodiment 3

[0093] Prescription three:

[0094] According to the prescription volume 1ml unit, the prescription is as follows:

[0095] Human Epidermal Growth Factor 1mg

[0096] Lecithin 89mg

[0097] Cholesterol (pharmaceutical grade) 11mg

[0098] Chloroform (analytical grade) 5ml

[0099] Phosphate buffer 1ml

[0100] Preparation process parameters:

[0101] Accurately weigh 89mg of lecithin, 11mg of cholesterol (PC: CHOL = 8: 1) dissolved in 5ml of chloroform ultrasonic (time 8min, frequency 53kHz) to dissolve, after mixing well, add 1ml hEGF aqueous solution (1mg / ml, drug substance ratio = 1: 100), ultrasonically mix for about 6 minutes to form a W / O emulsion, remove the organic solvent under reduced pressure (vacuum degree 800mbar, temperature 40°C) to form a gel-like substance, then add 1ml phosphate buffer (pH6.8), After ultrasonication for 10 min, 1 ml milky white homogeneous hEGF nanoliposomes were obtained.

[0102] Encapsulation efficiency result: 31.38%.

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Abstract

The invention discloses a human epidermal growth factor nanoliposome, which contains liposome microcapsules and human epidermal growth factor wrapped in the liposome microcapsules, wherein the mass ratio of the human epidermal growth factor to the liposome microcapsules ranges from 1: 50 to 1: 200. The invention also discloses a method for preparing the human epidermal growth factor nanoliposome. Phosphatide and cholesterol are adopted as the main membrane materials, whose biomembrane-like structures are similar to the skin structure and have good transdermal permeability, and are easier to prepare a lipid carrier with a uniform particles size and high entrapment rate by a preparation technology. According to the invention, human epidermal growth factor (hEGF) is prepared into the nanoliposome. As a raw material, the nanoliposome supplemented by other biologically-active substances such as hyaluronic acid is prepared by a corresponding preparation technology into aqua, cream and emulsion skin care products, so as to rapidly repair damaged skin, repair and remove wrinkles, nurse after sunscreen and radiation examination, nurse sensitive skin, and remove and repair various scars.

Description

technical field [0001] The invention relates to a cosmetic raw material and a preparation method, in particular to a liposome material and a preparation method thereof. Background technique [0002] Skin cell aging is the basis of skin diseases, so anti-aging is not only the fundamental issue of beauty, but also the key to skin health care. With age, the ability of basal cells to divide gradually decreases, while apoptosis increases with age. [0003] Human epidermal growth factor (hEGF), one of the human growth factors, is a small molecule polypeptide with a relative molecular weight of about 6200 consisting of 53 amino acids. Human epidermal growth factor (hEGF) is a powerful cell division factor with various biological activities. [0004] The main manifestations are: [0005] 1. Stimulate the growth and reproduction of skin tissue, cornea and tracheal epithelial tissue in vivo; [0006] 2. Accelerate the repair of epidermal wounds such as cornea and skin; [0007] 3...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/63A61K8/55A61K8/14A61Q19/08A61Q19/00A61Q17/04A61P17/02
Inventor 柯学赵学萃
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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