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Preparation method of high-purity salvianolic acid A

A salvianolic acid, high-purity technology, applied in the field of preparation of high-purity salvianolic acid A, can solve the problems of decreased growth, increased dead seedling rate, decreased number of roots, diameter and length, etc.

Inactive Publication Date: 2011-11-23
上海朗萨医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, salvia miltiorrhiza is not suitable for continuous cropping. Through the investigation of the growth and development of salvia miltiorrhiza in plots with different continuous cropping years from 1 to 4 years, it was found that continuous cropping seriously endangers the growth of salvia miltiorrhiza The quantity, diameter and length of the root system decreased, the yield decreased, the appearance of the root system was deformed, and the content of active ingredients decreased, which also affected the yield of Salvia miltiorrhiza to a certain extent.

Method used

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  • Preparation method of high-purity salvianolic acid A
  • Preparation method of high-purity salvianolic acid A
  • Preparation method of high-purity salvianolic acid A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1. (Taking 2000g comfrey as an example)

[0064] 1. Extraction: Weigh 2000g of comfrey (root and stem slices), heat and extract with 10 times the amount of water (20000ml) at 80°C for 2 hours, extract twice, combine and filter to obtain the extract.

[0065] 2. Transformation: Concentrate the extract to 4000ml, adjust the pH to 3 with 2mol / L hydrochloric acid, reflux for 7hr, and stop the reaction to obtain a transformation solution containing high-concentration salvianolic acid A.

[0066] 3. Separation: Weigh 2000g of DM301 macroporous resin, pretreat and pack into a column. Add the conversion liquid to the top of the chromatography column so that it completely enters the column bed. 5 column volumes were eluted with water to remove impurities, and then 3 column volumes were eluted with a solvent containing 70% ethanol, and these eluates were combined to obtain fractions containing salvianolic acid A.

[0067] 4. Purification: Concentrate the fractions under...

Embodiment 2

[0069] Example 2. (Taking 2000g comfrey as an example)

[0070] 1. Extraction: Weigh 2000g of comfrey (root and stem slices), heat and extract with 10 times the amount of aqueous solution at 100°C for 2 hours, extract twice, combine and filter to obtain the extract.

[0071] 2. Transformation: Concentrate the extract to 4000ml, adjust the pH to 5 with 0.5mol / L sulfuric acid, reflux for 5hr, and stop the reaction.

[0072] 3. Separation: Weigh 2000g of 330-type macroporous resin, pretreat and pack into a column. Add the conversion liquid to the top of the chromatography column so that it completely enters the column bed. 5 column volumes were eluted with water to remove impurities, and then 4 column volumes were eluted with a solvent containing 50% ethanol, and these eluates were combined to obtain fractions containing salvianolic acid A.

[0073] 4. Purification: After the fractions were concentrated to an appropriate volume, they were prepared and purified with a polyamide ...

Embodiment 3

[0074] Example 3. (Taking 1000g of comfrey as an example)

[0075] 1. Extraction: Weigh 1000g of comfrey (root and stem slices), heat and extract with 10 times the amount of aqueous solution at 85°C for 2 hours, extract twice, combine and filter to obtain the extract.

[0076] 2. Transformation: Concentrate the extract to 2000ml, adjust the pH to 6 with 0.2mol / L nitric acid, reflux for 24hr, and stop the reaction.

[0077] 3. Separation: Weigh 1000g of CAD45 macroporous resin, and pack it into a column after pretreatment. Add the conversion liquid to the top of the chromatography column so that it completely enters the column bed. 5 column volumes were eluted with water to remove impurities, and then 3 column volumes were eluted with 60% methanol, and these eluates were combined to obtain fractions containing salvianolic acid A.

[0078] 4. Purification: After the fractions were concentrated to an appropriate volume, they were prepared and purified with a Sephadex LH-20 colu...

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Abstract

The invention relates to a preparation method of high-purity salvianolic acid A. The preparation method is characterized in that: by taking lithospermum as a raw material, salvianolic acid A is obtained through the processes of water heating and extraction, acid regulation and transformation, resin absorption, elution and separation as well as purification and concentration. In the preparation method, the lithospermum is used as the raw material for preparing the salvianolic acid A, thereby expanding the sources of medicinal raw materials, saving the original salvia mitiorrhiza material and greatly reducing the use cost of the raw material; and simultaneously, the preparation method of the salvianolic acid A by taking the lithospermum as the raw material has a simple technological process and is suitable for large scale production, and the salvianolic acid A prepared by the method has high yield and purity and low cost.

Description

technical field [0001] The invention belongs to the field of plant medicines, in particular to a preparation method of high-purity salvianolic acid A. Background technique [0002] Salvia miltiorrhiza is a plant of the genus Salvia in the family Lamiaceae. As a commonly used Chinese medicinal material in my country, it has a long history of clinical application and plays an important role in the treatment of cardiovascular diseases. Its remarkable curative effect has been widely recognized clinically. The active chemical components of Danshen mainly fall into two categories: fat-soluble tanshinone compounds and water-soluble salvianolic acid compounds. Salvianolic acid has a wide range of anti-lipid peroxidation and free radical scavenging effects. Among the known phenolic compounds, salvianolic acid compounds have the strongest antioxidant activity. Among them, salvianolic acid A (referred to as salvianolic acid A ) has the strongest antioxidant activity. Salvianolic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56A61P9/00
Inventor 周长新甘礼社朱志彬
Owner 上海朗萨医药科技有限公司
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