Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sesame protein source metal chelating peptide and peptide trace element chelate and its application

A trace element, chelated peptide technology, applied in the application, hybrid peptide, peptide and other directions, can solve the problems of large intestinal content interference, low bioavailability, large gastrointestinal irritation, etc., to achieve a wide range of applications, raw material cost Low, easily absorbed effect

Inactive Publication Date: 2011-12-21
CHINA AGRI UNIV
View PDF1 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nutritional function of trace elements has gone through four stages of development. The first generation products are inorganic salt additives, such as copper sulfate, zinc sulfate, etc.; the second generation products are some simple organic acid salts, such as zinc gluconate, fumaric acid Ferrous iron, etc. These two types of products have poor stability, are highly irritating to the gastrointestinal tract, and are greatly disturbed by intestinal contents, poor absorption and utilization, and the total bioavailability is lower than 20%.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sesame protein source metal chelating peptide and peptide trace element chelate and its application
  • Sesame protein source metal chelating peptide and peptide trace element chelate and its application
  • Sesame protein source metal chelating peptide and peptide trace element chelate and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, enzymatic method prepares the sesame protein hydrolyzate containing metal chelating peptide

[0039] Method I

[0040] 1. Extraction of sesame protein by ultrasonic-assisted alkali-dissolving and acid-precipitation method

[0041] Pretreatment of sesame meal (purchased from Beijing Maozhiyuan Trading Co., Ltd.): fully pulverize the sesame meal, extract twice with petroleum ether, remove residual oil, and obtain pretreated sesame meal. Weigh 100g of the pretreated sesame meal, add 300mL of water, adjust the pH value to 11 with NaOH, ultrasonically extract for 1 hour, then centrifuge at 4000rpm for 20 minutes, collect the supernatant, and repeat the extraction twice. Combine the supernatants, adjust the pH to 4.0 with HCl, centrifuge at 8000 rpm for 15 minutes, collect the precipitate, and freeze-dry to obtain sesame protein.

[0042] 2. Preparation of sesame protein hydrolyzate containing metal chelating peptide by enzymatic method

[0043] The sesame pr...

Embodiment 2

[0064] Embodiment 2, preparation composite metal complexing peptide

[0065] 1. Separation and purification of metal chelating peptides

[0066] The freeze-dried powder obtained by method I in Example 1 is dissolved with a small amount of deionized water, separated with a metal chelating affinity chromatography column (zinc column), at first the zinc column is eluted with water, and what is separated is the metal chelating ability Poor peptides; then with concentrations of 0.3M, 0.6M and 0.9M 4 Cl 0.01M phosphate buffer (pH=6) was used as mobile phase gradient elution, the flow rate was 1ml / min, the temperature was room temperature, and the peptides in the mixture were eluted sequentially from weak to strong metal chelating ability, so that the peptides The mixture is divided into four components, detects the concentration of peptide at 254nm, and detects the corresponding metal chelating ability of each eluent (the assay method of metal chelating ability is the same as in Ex...

Embodiment 3

[0071] Embodiment 3, preparation metal chelating peptide pure substance

[0072] 1. Preparation of metal chelating peptide NCS

[0073] The complex metal-complexing peptide prepared in Example 2 above was separated and purified by reverse-phase high-performance liquid chromatography (HPLC). When separated by HPLC, use ODS column (YMC ODS-AQ S-5 4.6×250mm, USA). The sample volume was 20 μL. The elution solvents are: A, pure water (v / v) containing 0.1% trifluoroacetic acid (TFA); B, 80% acetonitrile solution added with 0.1% TFA. Gradient elution was performed with a mixture of A and B, (volume percentage of solvent B from 10% to 60%). Flow rate: 1.0ml / min, elution time 30 minutes. Detect the peak position of the peptide at 210nm, such as figure 2 shown, where figure 2 A is figure 1 Middle component 2 is through the spectrogram that reverse phase high-performance liquid chromatography (RP-HPLC) separates; Collect and measure the metal chelating ability of the eluent co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a sesame protein source metal chelating peptide and a peptide trace element chelate and application thereof. The invention provides a polypeptide mixture. The polypeptide mixture provided by the invention is prepared by a method comprising the following steps of: hydrolyzing sesame protein by using protease to obtain a hydrolysis product containing polypeptide with amino acid sequence Asn-Cys-Ser, and thus obtaining the polypeptide mixture. According to the prepared sesame protein source metal chelating peptide and the peptide trace element chelate, the raw material cost is low; sesame seed meal is generally used as a raw material for preparing feed, so the finished product has high additional value; the metal chelating peptide provided by the invention contains 2 to 3 amino acids, has low molecular weight, and is directly absorbed by the digestive tract more easily; and the production technology has low equipment requirement and low investment, and is easy in development and utilization.

Description

technical field [0001] The invention relates to a sesame protein source metal chelating peptide and a peptide trace element chelate and application thereof. Background technique [0002] Trace elements are important nutrients for maintaining life activities and growth and development. The nutritional function of trace elements has gone through four stages of development. The first generation products are inorganic salt additives, such as copper sulfate, zinc sulfate, etc.; the second generation products are some simple organic acid salts, such as zinc gluconate, fumaric acid Ferrous iron, etc. These two types of products have poor stability, are highly irritating to the gastrointestinal tract, are greatly disturbed by intestinal contents, and are poorly absorbed and utilized. The total bioavailability is lower than 20%. The third-generation product, amino acid trace element chelate salt, has the advantages of strong anti-interference, good stability, and easy absorption, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K19/00C07K5/093C12P21/06A23K1/16A23L1/305A23K20/147A23K20/20A23L33/165A23L33/18
Inventor 李博汪婵
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com