New compound separated from Mythic Fungus, preparation method thereof and medicinal purpose thereof

A compound and drug technology, applied in the field of new compounds for multi-drug resistance (MDR), can solve problems that have not been widely used

Active Publication Date: 2012-01-11
福建仙芝楼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional Chinese medicine has been used to improve the general condition of cancer patients, but it has not been widely used for the specific eradication of MDR cancer cells

Method used

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  • New compound separated from Mythic Fungus, preparation method thereof and medicinal purpose thereof
  • New compound separated from Mythic Fungus, preparation method thereof and medicinal purpose thereof
  • New compound separated from Mythic Fungus, preparation method thereof and medicinal purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 compound (1) preparation

[0030] The material source Ganodema Lucidum (Leys.ex Fr.) KARST) is purchased from China Fujian Xianzhi Tower Biotechnology Co., Ltd., which is stored at the School of Pharmacy of Fujian Medical University.

[0031]Extract and separate dry and crushed ganoderma with water -free ethanol to extract 3 times, 2h each time, filter with No. 2 filter paper, and remove ethanol with a rotating evaporator to get alcohol extraction.After adding an appropriate amount of water to the alcohol, use petroleum ether and ethyl acetate to extract in turn.Perform the column layer on the silicone, elute it with chloroform-methanol gradient, and take chloroform-methanol (volume ratio 99: 1) to cricket column layer.Ether-ethyl acetate (volume ratio ratio 4: 1) to elute, take this part of the preparation of high-efficiency liquid chromatography (Shimadzu LC-6AD semi-preparation high-efficiency liquid chromatography instrument), methanol-water gradient to eluted...

Embodiment 2

[0032] Example 2 compounds (1) chemical structure determination

[0033] Structural measurement is used to record the FTir spectrum with Perkin-Elmer 1600 optical light meter, and the ultraviolet spectrum is measured with Shimadzu-3100 Lighting Turn. 3 In the solution, the Bruker MRI can record the NMR spectrum, and use the Agilent 6210 flight timermia.

[0034] The physical and chemical properties of compounds (1) are the invention compounds (1) are white and foster powder, UV (ETOH) λmax 254nm; Liebermann-Burchard reaction positive; IR νmax (CM -1 ): 3427, 2971, 2917, 2888, 1725, 1703, 1652, 1453, 1408, 1380, 1306, 1239, 1056; 1 H-nmr (CDCL 3 , 500MHz): Δ1.52 (1H, M, H-1), Δ2.90 (1H, M, H-1 '), Δ2.50 (1H, M, H-2), Δ2.45M, H-2 '), Δ1.55 (1H, M, H-5), Δ2.11 (1H, M, H-6), Δ1.64 (1H, M, H-6'), Δ4.81 (1H, M, H-7), Δ2.79 (1H, D, J = 8.5Hz, H-12), Δ2.75 (1H, D, J = 8.5Hz, H-12 '), Δ2.12 (1H, DD, J = 19.5, 9.5 Hz, H-16), Δ2.79 (1H, DD, J = 19.5, 8.5Hz, H-16 '), Δ1.97 (1H, M, H-17), Δ0.9...

Embodiment 3

[0037] Example 3 compounds (1) Biology experiments and analysis of anti -cancer effects

[0038] 1. Materials and methods

[0039] Cell and reagent high-expressed drug-resistant cells K562 / A02, human chronic granulocyte leukemia cells K562, human marrow leukemia cell plant HL60, human Burkitt lymphoma cell CA46, human colon cancer cell strain SW1116Human colon cancer cells SW480, human liver cancer cells SMMC7221, and human liver cancer cells HEPG2.Cultivate these cells in the RPMI 1640 culture liquid containing 10%calf serum, set 37 ° C, 5%saturated humidity CO 2 Cultivation in the culture box (SMMC7221 of human liver cancer cells and human colon cancer cells SW480 are cultivated with 10%Maverick serum culture liquid).The above compounds (1) chemical names are Achide Athyl, which are obtained from the preparation of Example 1.

[0040] Cell proliferation analysis takes the number of K562, HL60, CA46, SW1116, SW480, SMMC7221 and HEPG2 cells, and inoculate in a certain density in t...

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PUM

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Abstract

The invention relates to a new compound separated from Mythic Fungus, a preparation method thereof and a medicinal purpose thereof. The invention discloses a compound with the chemical structural formula shown in the specification. The preparation method of the compound comprises the following steps: 1, extracting Mythic Fungus with alcohol or an alcoholic solution once or more than twice, filtering, collecting a filtrate, carrying out reduced pressure concentration, and drying to obtain an alcohol extract; 2, adding water to the alcohol extract, extracting with petroleum ether for degreasing, extracting with ethyl acetate, extracting an obtained ethyl acetate extract with an aqueous solution of NaHCO3, taking an ethyl acetate phase, and evaporating to dryness to obtain a crude product; and 3, carrying out chromatogram separating separation and purification on the obtained crude product to obtain the compound. The compound of the present invention can be treated as a medicine for treating tumors.

Description

Technical field [0001] The present invention involves a new compound that has a multi -resistant drug (MDR) that is separated from ganoderma and can inhibit tumor cells. [0002] The invention also involves the preparation of the compound. [0003] The invention also involves the application of the compound in the preparation of the treatment of tumor drugs. Background technique [0004] Muti-Drug Resistance (MDR) of the tumor is clinically considered to be the most common cause of chemotherapy failure. The longer chemotherapy for tumors often leads to the selective survival of cancer cells. These surviving cancerCells have cross -resistant chemotherapy drugs that are unrelated to broad -spectrum.Several mechanisms that may lead to MDR include the failure to enter a programmatic death (apoptosis) or the drug failed to reach or act on the intracellular target due to the adaptation of chemotherapy.Anti -cancer therapy, such as drug treatment, biological therapy, or radiation therap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00A61K31/575A61P35/00A61P35/02
Inventor 许建华李鹏张志强
Owner 福建仙芝楼生物科技有限公司
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