Method for recovering alhumin from deposited components I+II+III from cold ethanol method

A low-temperature ethanol method, albumin technology, applied in the preparation methods of albumin peptides, serum albumin, peptides, etc., can solve the problem of low albumin extraction rate, improve the total yield, reduce production costs, improve quality effect

Active Publication Date: 2012-01-11
XIAN HUITIAN BLOOD PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a production process for recovering albumin from the precipitation of components I+II+III by the low-temperature ethanol method, which solves the problem that the extraction rate of albumin in the prior art is not high

Method used

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  • Method for recovering alhumin from deposited components I+II+III from cold ethanol method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Preparation of F1 supernatant

[0030] Use 5 to 6 times the volume of water for injection to dissolve the precipitate of component I+II+III, stir at 1°C for 3 hours to completely dissolve it, add sodium dihydrogen phosphate to 0.01mol / L, and then add 0.2 mol / L sodium hydroxide to adjust the pH value to 4.95, slowly add ethanol with a volume fraction of 95% to make the volume concentration of ethanol in the solution reach 7.5%, and control the solution temperature to -4.5°C to cause F1 precipitation; after the F1 precipitation is completely produced Add perlite to 2.8g / L and diatomaceous earth to 2.8g / L, continue to stir to make the liquid into a slurry, press filter to separate the FI precipitate produced in the system, and obtain the FI supernatant;

[0031] (2) Preparation of FⅢ supernatant

[0032] Transfer the FⅠ supernatant from the previous step into a clean stainless steel reaction tank, add 0.2 mol / L sodium hydroxide to adjust the pH to 5.10, continue to ad...

Embodiment 2

[0046] (1) Preparation of F1 supernatant

[0047] Use: 6 times the volume of water for injection to dissolve the component I+II+III precipitate, stir at 5°C for 5 hours to completely dissolve it, add sodium dihydrogen phosphate to 0.01mol / L, adjust the pH value to 5.5, and slowly add The volume fraction of ethanol is 95% so that the volume concentration of ethanol in the solution reaches 8.5%, and the temperature of the solution is controlled between -2°C to cause F1 precipitation; after the F1 precipitation is complete, add perlite to 3.2g / L, silicon Alginate 3.2g / L, continue to stir to make the liquid into a slurry, press filter to separate the FI precipitate produced in the system, and obtain the FI supernatant;

[0048] (2) Preparation of FⅢ supernatant

[0049] Transfer the FⅠ supernatant from the previous step into a clean stainless steel reaction tank, adjust the pH value to 5.2, continue to add ethanol to a volume concentration of 14.5%, and control the liquid tempera...

Embodiment 3

[0063] (1) Preparation of F1 supernatant

[0064] Use 5.5 times the volume of water for injection to dissolve the precipitate of component I+II+III, stir at 3°C ​​for 4 hours to dissolve completely, add sodium dihydrogen phosphate to 0.01mol / L, adjust the pH value to 5.00, and slowly add volume Ethanol with a fraction of 95% makes the volume concentration of ethanol in the solution reach 8.0%, and the temperature of the solution is controlled between -2.2°C to cause F1 precipitation; after the F1 precipitation is complete, add perlite to 3.0g / L, diatom Soil 3.0g / L, continue to stir to make the liquid into a slurry, press filter to separate the FI precipitate produced in the system, and obtain the FI supernatant;

[0065] (2) Preparation of FⅢ supernatant

[0066] Transfer the FⅠ supernatant from the previous step into a clean stainless steel reaction tank, adjust the pH value to 5.15, continue to add ethanol to a volume concentration of 14%, and control the liquid temperature...

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Abstract

The invention relates to a method for recovering alhumin from deposited components I+II+III from the cold ethanol method. Traditional recovery methods have defects of less treating capacity of a chromatography column, large investment in solid-liquid centrifugation equipment and high energy consumption; in addition, by traditional recovery methods, only gamma globulin of the components is extracted during the extraction process, and an FII supernatant still contains much alhumin, thus influencing the extraction efficiency. The technical scheme adopted in the invention comprises the following steps of: (1) preparation of an F1 supernatant; (2) preparation of an FIII supernatant; (3) preparation of a crude alhumin solution; (4) extraction of FIII alhumin deposit; (5) preparation of V deposit; (6) impurity removal of a protein solution; and (7) preparation of an alhumin product. By the adoption of the method provided by the invention, alhumin remained in the deposited components I+II+IIIis effectively recovered, thus increasing the extraction amount of alhumin per ton blood plasma raw material by 0.5-1.0 kilograms, raising the total yielding rate of alhumin and reducing the production cost.

Description

Technical field [0001] The present invention is the field of blood products technology, involving a method of extracting recycling and recycling of leukin from the low -temperature ethanol component Ⅰ+Ⅱ+Ⅲ sedimentation. Background technique [0002] Extracting the separation of albumin from plasma is carried out at home and abroad. At home and abroad, the low -temperature ethanol method based on the COHN6 method is based on the difference in the physical and chemical properties of plasma protein.The conditions affecting protein stability are precipitated to extract different proteins, respectively. [0003] There are five main factors that affect protein precipitation in the low temperature ethanol method, namely pH, temperature, protein concentration, ionic intensity, ethanol concentration.By controlling these parameters, the different protein components in plasma can be precipitated step by step. Generally, the component I+Ⅱ+III is generally precipitated from the plasma, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/36C07K1/30C07K1/18
Inventor 张林张卫柱张华平张云郭军峰
Owner XIAN HUITIAN BLOOD PROD CO LTD
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