Screw ring alkaloid compound, preparation method thereof and application in the aspect of marine organism fouling resistance

A technology of marine biofouling and compounds, applied in the field of anti-marine biofouling, the field of preparation of alkaloid compounds, can solve the problems of affecting embryo growth, low release rate, destroying ecological balance, etc., and achieve inhibition of barnacle larvae Effects of attachment to bryozoa larvae, simple preparation method, and environmental friendliness

Inactive Publication Date: 2013-06-19
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequent antifouling coatings that dominate the market are copper-containing antifouling coatings with low release rates. However, heavy metal elements will accumulate in the ocean, especially in harbors, resulting in a large number of algae deaths, affecting carp, shrimp, and crabs. And the embryonic growth of various molluscs and backpack animals, destroying their blood cells, thereby destroying their ecological balance, and will eventually be banned

Method used

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  • Screw ring alkaloid compound, preparation method thereof and application in the aspect of marine organism fouling resistance
  • Screw ring alkaloid compound, preparation method thereof and application in the aspect of marine organism fouling resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Sorbitol 20g, yeast extract 3g, MgSO 4 ·7H 2 O 0.3g, MSG 10g, Tryptophan 0.5g, KH 2 PO 4 0.5 g, 100 g of maltose, and 10 g of NaCl were mixed, and the volume was adjusted to 1 L with water, and the pH was adjusted to 6.5 to obtain No. 2 medium. Put the No. 2 medium into about 100 500mL Erlenmeyer flasks, each bottle is about 150mL, and sterilize at 115°C for 25 minutes by high-pressure steam.

[0024]Use a pipette gun to inoculate about 2 microliters of the marine fungus Penicillium sp.Scsio.00776 CCTCC NO: M 2011147 into the agar-potato plate medium (the medium is 200 grams of potatoes, 20 grams of glucose, 30 grams of sea salt, 20 gram agar and 1 liter of pure water), cultivated at 28°C for 3 days to obtain a plate with cultured strains, and then picked about 3 microliters of strains from the plate with a bamboo stick to inoculate into 150 mL of the above-mentioned 2 No. culture medium in Erlenmeyer flask in shaker (rotating speed 200r / min) temperature 28 ℃ and c...

Embodiment 2

[0027] Embodiment 2: Anti-biological barnacle larva attachment test of formula (1) compound

[0028] The activity test model adopts the attachment inhibition test of Barnacles larvae, one of the most commonly used marine fouling biological models in the laboratory. Adult barnacles feed on Chaetoceros gracilis Schutt, and culture barnacle nauplii to Venus larva stage in 0.22 μm filtered seawater, with 2 larvae per ml at 28°C. The resulting Venus stage larvae were tested for Venus activity. Anti-larval settling activity of compounds was determined using 24-well polystyrene plates. The pure product obtained in Example 1 was dissolved in DMSO, and diluted with sterile filtered seawater to obtain different concentration solutions (60-140 μg / mL). Add 1mL test solution and 20±2 mature barnacle larvae to each well, set 5 replicate samples for each concentration, and sterile filtered seawater as blank control. Place the 24-well cell culture plate in a 30°C incubator for 24 hours. T...

Embodiment 3

[0030] Embodiment 3: Anti-adhesion activity test of the compound of formula (1) against the larvae of marine macrofouling organisms

[0031] The activity test model adopts the attachment inhibition test of Burgelaneritina larvae, one of the most commonly used marine fouling biological models in the laboratory. The collected mature B. multiloculum was placed in seawater filtered with a 0.22 μm filter membrane, stimulated with light, and the larvae produced by the mature B. multiloculum stimulated by light were collected. Larvae were placed in a watch glass containing seawater filtered through a 0.22 μm filter membrane. The activity test was done with the larvae produced by Bryosa larvae by light. Anti-larval settling activity of compounds was determined using 24-well polystyrene plates. The pure compound was dissolved in DMSO and diluted with sterile filtered seawater to obtain different concentration solutions (60-140 μg / mL). Add 1mL test solution and 20±2 mature barnacle l...

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Abstract

The invention relates to a novel screw ring alkaloid compound, a preparation method thereof, and the application in the aspect of marine organism fouling resistance. The invention is characterized in that the structure of the screw ring alkaloid compound is expressed in formula (1). The preparation method comprise the steps that marine fungus Penicillium sp. Scsio. 00776 CCTCC NO: M 2011147 is fermented, obtained fermentation liquor is extracted with ethyl acetate and other solvents, through antiphase silica gel column chromatography, obtained solvent extractives are gradiently eluted sequentially with water-methanol systems with the methanol volume fractions of 5%, 35%, 55%, 75% and 100%, components flushed by taking water-methanol with the methanol volume fraction of 55% as an eluent are collected, sephadex column chromatograph is carried out on the components to obtain crude products, and the crude products are finally purified to obtain the screw ring alkaloid compound. The screw ring alkaloid compound disclosed by the invention has the activity of inhibiting bugula larva and kentrogon attachment, has the function of resisting marine organism fouling, and can be used for preparing efficient marine antifouling agents. Formula (1) is disclosed in the specification.

Description

technical field [0001] The present invention relates to an alkaloid compound, specifically to a novel spirocyclic alkaloid compound, and also to a preparation method of the alkaloid compound and its application in anti-marine biofouling. Background technique [0002] In the historical process of developing and utilizing the ocean, human beings have always been faced with the problem of preventing and eliminating marine organisms. Marine fouling organisms are a general term for various animals, plants and microorganisms that inhabit or attach to ships and various underwater artificial facilities and adversely affect human economic activities. There are more than 4,000 species of marine fouling organisms in the world, and 614 species have been recorded in my country's coastal areas, among which the most important groups are algae, hydra, external anal animals, eucalyptus, bivalves, barnacles, and sea squirts. Marine fouling organisms attach, grow and even form communities on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/20C09D5/16C12P17/18C12R1/80
Inventor 漆淑华贺飞
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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