Solid-state fermentation method for bacillus natto-saccharomycete composite viable bacteria preparation with rice bran as matrix

A technology of Bacillus natto and solid-state fermentation, which is applied in the field of bioengineering, can solve problems such as limited effects, and achieve the effects of low production cost, good probiotic effect, and good probiotic effect

Inactive Publication Date: 2012-02-01
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the probiotics of a single strain of microecology contain less probiotics, the effect is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of fermentation medium: use full-fat rice bran and soybean meal as raw materials, which should be dry without agglomeration, mildew, deterioration, and uniform texture. 90 kg of rice bran, 8 kg of soybean meal, 2 kg of glucose, and 60 kg of water were added to keep the material fully moist without agglomeration, and the pH value of the material was adjusted to 6.0 to 6.5 with 1 N NaOH; fermented in a solid-state fermentation tank, and the fermentation medium was sterilized in a solid tank. The sterilization conditions are 115-125 °C / 20-40 min, and cooled to 30-37 °C. spare. Or use box-type fermentation, the fermentation medium is sterilized separately, the sterilization conditions are 115-125 ℃ / 20-40 min, and cooled to 30-37 ℃. spare.

[0029] (2) Strain preparation: The slant surface of the production strain was activated and passaged twice to improve the vitality of the strain. Primary seed culture: Take 1 ring of Bacillus natto from the slope with ...

Embodiment 2

[0033] Fermentation medium preparation: defatted rice bran and soybean meal raw materials should be dry without agglomeration, mildew, deterioration and uniform texture. 85 kg of rice bran, 10 kg of soybean meal, 5 kg of glucose, and 60 kg of water. In the solid-state fermentation method, the inoculation ratio of fermentation strains is Bacillus natto seed liquid: Saccharomyces cerevisiae seed liquid=7:3, inoculum size 8.0kg, and other steps and process parameters are the same as in Example 1.

Embodiment 3

[0035] (1) Preparation of fermentation medium: use full-fat rice bran and soybean meal as raw materials, which should be dry without agglomeration, mildew, deterioration, and uniform texture. 95 kg of rice bran, 10 kg of soybean meal, 5 kg of glucose, and 90 kg of water were added to keep the material fully moist without agglomeration, and the pH value of the material was adjusted to 6.0 to 6.5 with 1 N NaOH; fermented in a solid-state fermentation tank, and the fermentation medium was sterilized in a solid tank. The sterilization condition is 125 ℃ / 40 min, cooled to 37 ℃, and set aside.

[0036] (2) Strain preparation: The slant surface of the production strain was activated and passaged twice to improve the vitality of the strain. Primary seed culture: Take 1 ring of Bacillus natto from the slope with an inoculation needle, insert it into a 250 mL Erlenmeyer flask filled with 30 mL of beef extract protein medium, and culture it with shaking at 37 °C and 140 r / min 12-16 hou...

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Abstract

The invention discloses a solid-state fermentation method for bacillus natto-saccharomycete composite viable bacteria preparation with rice bran as a matrix, which includes the following steps: fermentation medium preparation: rice bran and soya bean meal are chosen as main materials, and are weighed according to a fermentation medium formula, the components of the medium are 85 to 95 percent by weight of the rice bran, 2 to 10 percent by weight of the soya bean meal, 1 to 5 percent by weight of glucose and the balance of water, the materials are kept sufficiently moist, and are not agglomerated, the pH value of the materials is regulated to 6.0 to 6.5 by NaOH solution, and the materials are sterilized for later use; solid-state fermentation: as for the inoculation proportion of the fermentation strain, the weight ratio of bacillus natto seed fermentation broth to saccharomyces cerevisiae seed fermentation broth is (5 to 7):(5 to 3), the inoculation proportion is 5 to 10 percent by weight, the solid-state fermentation time is 4 to 6 days, and thereby the composite viable bacteria preparation is obtained; and composite viable bacteria preparation drying and packaging are carried out. The composite viable bacteria preparation has the characteristics of high viable count, low cost, simple process, little investment, low energy consumption, no waste water, little environment pollution, convenient post-processing and the like.

Description

technical field [0001] The invention relates to a solid-state fermentation method for a compound feed bacterial agent of bacillus natto and yeast using rice bran as a substrate, and belongs to the technical field of bioengineering. Background technique [0002] Probiotics are living bacteria preparations made from beneficial microorganisms isolated from humans, animals, and plants under the guidance of microecology theory, and made through cultivation, fermentation, and drying processes. [0003] At present, 42 kinds of microbial strains are allowed to be directly fed in the world (U.S. Food and Drug Administration, American Feed Association, 1989), and the Ministry of Agriculture of my country announced in 1999 that there are 12 kinds of feed-grade microbial additive strains that are allowed to be used, including cheese Lactobacillus ( L. casei ), Lactobacillus plantarum ( L. plantarum ), Streptococcus faecalis ( S. faecalis ), Streptococcus faecium ( S.faecium ), Ped...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12N1/18C12R1/07C12R1/865A23K10/12A23K10/37A23K20/163
CPCY02P60/87
Inventor 黄占旺牛丽亚帅明蔡凯凯
Owner JIANGXI AGRICULTURAL UNIVERSITY
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