Chitosan artificial nerve graft containing neurotrophic factor and preparation method thereof

A technology of neurotrophic factors and artificial nerves, which is applied in the field of medical devices, can solve the problems of limited tensile strength of nerve conduits, large product apertures, and cumbersome steps, and achieve the effects of long-term storage and transportation, guaranteed activity, and a good growth environment

Inactive Publication Date: 2012-02-08
JIANGSU YITONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires multiple stainless steel needles of a certain diameter to be fixed in parallel in the chitosan tube. The steps are cumbersome and the product has a large pore size, and the tensile strength of the nerve guide is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Chitosan was dissolved with lactic acid with a concentration of 0.5% to obtain a solution with a concentration of 3%. Then the solution was injected into the mold, and after pre-forming by freezing at -60°C, the material was withdrawn from the mold and put into 5% sodium hydroxide solution to neutralize the acid in the material. After cleaning, it was placed in a 15 μg / ml nerve growth factor (NGF) solution and fixed for 30 minutes using the abundant pores on the surface of the material. The chitosan artificial nerve graft containing neurotrophic factor is then obtained by freeze-drying, with a porosity of 89% and an average pore diameter of 87-98 μm.

Embodiment 2

[0028]Chitosan was dissolved with 8% acetic acid to obtain a 5% chitosan solution. Then the solution was injected into the mold, and after pre-forming by freezing at -80°C, the material was withdrawn from the mold and put into a 10% sodium carbonate solution. After the acid in the neutralized material was fixed and molded, the excess alkali and the resulting salt were removed with distilled water. After cleaning, the chitosan artificial nerve graft is obtained by freeze-drying. Before use, the chitosan artificial nerve grafts were placed in a 20 μg / ml solution of basic fibroblast growth factor (bFGF) and fixed for 1 hour using the abundant pores on the surface of the material. The chitosan artificial nerve graft containing neurotrophic factor is then obtained by freeze-drying, with a porosity of 77% and an average pore diameter of 55-67 μm.

Embodiment 3

[0030] Dissolve chitosan with phosphoric acid with a concentration of 2% to obtain a solution with a concentration of 6% chitosan, and then inject the solution into the mold, after pre-forming by freezing at -90 °C, the material is withdrawn from the mold and placed in 5% sodium hydroxide In the solution, after the acid in the neutralized material is fixed and formed, the excess alkali and the generated salt are washed with distilled water, and then freeze-dried to obtain the chitosan artificial nerve graft. Before use, the chitosan artificial nerve grafts were placed in a 20 μg / ml brain-derived neurotrophic factor (BDNF) solution and fixed for 2 hours using the abundant pores on the surface of the material. Then, the chitosan artificial nerve graft containing neurotrophic factor is obtained by freeze-drying, with a porosity of 53% and an average pore diameter of 11-23 μm.

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Abstract

The invention discloses a chitosan artificial nerve graft containing a neurotrophic factor and a preparation method thereof. The chitosan artificial nerve graft is prepared by processing chitosan into a nerve conduit with a porous structure and high tensile strength by virtue of weak acid dissolution, mold injection, freezing formation, neutralization and immobilization, cleaning, lyophilization and other processes under a condition of not adding any foaming agent or cross linking agent. The cell factor with a treatment effect in the nerve conduit is one or more of a basic fibroblast growth factor (bFGF), a brain derived neurotrophic factor (BDNF), a neurotrophic factor 3 (NT-3), a basic fibroblast cell growth factor (bFGF) and a glial-cell-line derived neurotrophic factor (GDNF) and the like. The product provided by the invention contains the neurotrophic factor, adopts degradable materials, and has excellent biocompatibility with a human body. The prepared product does not contain exogenous toxic or side-effect substances brought by a preparation process.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to an artificial nerve graft containing a neurotrophic factor and a preparation method thereof. Background technique [0002] In the real society, events such as traffic accidents, industrial accidents, sports accidents, and clinical operations can cause peripheral nerve damage. In clinical practice, when long-distance nerve defects cannot be compensated for by end-to-end sutures, they have to rely on grafts. to bridge repair. In recent years, tissue engineering based on the principles and technologies of life science and engineering has provided a new way to construct substitutes for nerve transplantation. The research on using tissue engineering technology to repair nerve injury mainly focuses on the design and construction of new tissue-engineered nerve bridging devices. At present, such bridging devices are usually made of biomaterials alone, and are processed into tubular struct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/54A61L27/56C08J9/40C08J9/28
Inventor 杨宇民张添顾晓松丁斐
Owner JIANGSU YITONG BIOTECHNOLOGY CO LTD
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