Method for preparing high-purity poliumoside

A high-purity technology of chrysoside, applied in the field of natural medicinal chemistry, can solve the problems of large amount of solvent and small preparation amount, and achieve the effects of large preparation amount, high degree of industrialization and high purity

Inactive Publication Date: 2012-02-15
NANJING ZELANG MEDICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But adopt preparation liquid phase to prepare chrysoside, th

Method used

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  • Method for preparing high-purity poliumoside

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Embodiment 1

[0016] Pulverize the medicinal material of Guangdong Zizhu, take 2kg and add 5 times the amount of 70% acetone solution for ultrasonic extraction (200w, 40KHz) 3 times, 30 minutes each time, the extract is filtered to recover the solvent, the concentrated solution is added to the same volume of water-saturated n-butanol for extraction 2 The second time, the extract was perfused into a short column of alumina (500g of neutral alumina, with a particle size of 120-200 mesh), and the column solution was concentrated under reduced pressure to obtain 35g of extract. Chloroform, methanol, isopropanol, and aqueous solution are mixed at a ratio of 3:1:0.5:5. After fully stratified, remove the lower phase and fill the high-speed countercurrent chromatography column, start the host, adjust the speed to 700rpm, and pump the upper phase as the mobile phase at the same time. After dynamic balance, the flow rate was adjusted to 2ml / min, the mobile phase was dissolved and the extract was injec...

Embodiment 2

[0018] Pulverize the medicinal material of Guangdong Zizhu, take 2kg and add 5 times the amount of 50% acetone solution for ultrasonic extraction (200w, 60KHz) 3 times, 30 minutes each time, the extract is filtered to recover the solvent, and the concentrated solution is added to the equal volume of water-saturated n-butanol for extraction 2 The second time, the extract was perfused into a short column of alumina (500g of neutral alumina, particle size 120-200 mesh), and concentrated under reduced pressure to obtain 42g of extract. Chloroform, methanol, isopropanol, and aqueous solution are mixed according to the ratio of 10:4:2:15. After fully stratified, take the lower phase and fill the high-speed countercurrent chromatography column, start the host, adjust the speed to 1000rpm, and pump the upper phase as the mobile phase at the same time. After dynamic balance, the flow rate was adjusted to 3ml / min, the mobile phase was dissolved and the extract was injected with a samplin...

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Abstract

The invention discloses a method for preparing high-purity poliumoside. The method comprises the following steps of: smashing callicarpa kwangtungensis serving as a medicinal material; adding a 50-70 percent acetone solution in an amount which is 5-7 times that of the medicinal material for performing ultrasonic extraction for 2-3 times; filtering the extracting solution to recover a solvent; adding a concentrated solution into water-saturated n-butyl alcohol for extracting; making the extraction liquid pass through a short aluminum oxide column; recovering a reagent from the column passing liquid; separating the obtained extract by adopting a high-speed counter-flow chromatography; detecting with an ultraviolet detector; collecting a target component; and drying under reduced pressure to obtain a poliumoside monomer. The method for preparing the high-purity poliumoside has simple process steps, large preparation amount and a short period, and the purity of the obtained product is not less than 98 percent.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a preparation method of high-purity aureoside. Background technique [0002] Guangdong Purple Pearl is a plant of the Verbenaceae Purple Pearl, which is used as medicine with stems and leaves. Have astringent to stop bleeding, dissipate blood stasis, the effect of heat-clearing and toxic substances removing. Commonly used for epistaxis, hemoptysis, hematemesis, hematochezia, metrorrhagia, traumatic bleeding, cough due to lung heat, sore throat, toxic heat sores, water and fire burns. Modern studies have found that purple pearls contain flavonoids, phenylpropanoid glycosides, polysaccharides and volatile oils. "Pharmacopoeia of the People's Republic of China"-2010 Edition stipulates that the total amount of forsythiaside B (C34H44019) and silicoside (C35H46019) in Guangdong Zizhu shall not be less than 0.50%. [0003] Poliumoside is a phenylpropanoid glyco...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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