Preparation method of rice bran nutrient

A nutrient and rice bran technology, which is applied in the field of rice bran nutrient preparation, can solve the problems of high production equipment requirements, waste of resources, and small production scale, and achieve low production equipment requirements, enhance immunity, and prevent obesity.

Inactive Publication Date: 2012-02-22
ANHUI YANZHIFANG FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the total output of domestic rice bran is large, due to the immature deep processing technology of rice processing enterprises, the production scale is not large, and the stability of fresh rice bran is poor, it is not easy to store and transport, so it is difficult to concentrate production
At present, the effective utilization rate of rice bran is still less than 20%, and most of them are used as feed, or even as waste, which is a serious waste of resources.
[0009] In short, the deep processing of rice bran at home and abroad mainly focuses on the technology of separately extracting nutrients such as rice bran protein, rice bran polysaccharide, and rice bran fiber. Give full play to the role of various nutrients in rice bran in dietary health care

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Extraction and separation of rice bran nutrients

[0031] (1) Take fresh rice bran, the total nitrogen content of rice bran is 14.4%, the fat content is 15.2%, and the water content is 18.5%; 1000g rice bran is washed, dried, crushed, passed through a 80-mesh sieve, and treated with humid heat and high-temperature steam at 85°C for 6 minutes, and Gradually raise the temperature to 105°C within 12 minutes, and keep warm for 15 minutes;

[0032] (2) Add 2645g of petroleum ether to the material obtained in step (1), stir at 55-58°C for 0.6h, filter, and dry the filter residue at 105°C for 1h;

[0033] (3) Mix the material obtained in step (2) with aqueous hydrochloric acid solution of pH 5.5 at a material-to-liquid weight ratio of 1:8, add 20g of combined enzyme A, maintain 40°C and stir for 1 hour, centrifuge at 3500r / min, and collect Serum;

[0034] (4) Add 15g of combined enzyme B to the supernatant obtained in step (3), maintain 55°C and stir for 1-2h, cent...

Embodiment 2

[0037] Example 2 Extraction and separation of rice bran nutrients

[0038] (1) Take fresh rice bran, the total nitrogen content of rice bran is 14.6%, the fat content is 15.3%, and the water content is 18.3%; 1000g rice bran is washed, dried, crushed, passed through a 80-mesh sieve, and treated with humid heat and high-temperature steam at 85°C for 8 minutes, and Gradually raise the temperature to 105°C within 15 minutes, and keep warm for 15 minutes;

[0039] (2) Add 2237g of petroleum ether to the material obtained in step (1), stir at 55-58°C for 1h, filter, and dry the filter residue at 105°C for 1h;

[0040] (3) Mix the material obtained in step (2) with aqueous hydrochloric acid solution of pH 5.0 at a material-to-liquid weight ratio of 1:10, add 20g of combined enzyme A, maintain 45°C and stir for 1 hour, centrifuge at 4000r / min, and collect Serum;

[0041] (4) Add 15g of combined enzyme B to the supernatant obtained in step (3), maintain 60°C and stir for 1-2 hours, ...

Embodiment 3

[0046] Example 3 Adjuvant Therapeutic Effect of Rice Bran Nutrients on Liver Cancer Mouse Model

[0047] SPF grade healthy Kunming mice, male and female, 6 weeks old, weighing 18g-20g. Take H22 (S-type) liver cancer cells in the logarithmic growth phase cultured in vitro, and adjust the concentration of tumor cells to 1×10 7 / mL. Under sterile conditions, the tumor cells were inoculated subcutaneously in the right axilla of mice, with an inoculation volume of 0.2ml / mouse (the number of cells was 2×10 6 / Only). The entire inoculation process must be carried out in a sterile hood with aseptic operation, and the inoculation should be completed within 1 hour. The volume of the subcutaneous transplanted tumor reaches 60mm 3 Around time (about lOd), the model was successfully manufactured. The liver cancer mice were divided into 4 groups according to the principle of tumor volume and weight balance of tumor-bearing mice: model control group, cyclophosphamide group, test group I...

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PUM

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Abstract

The invention discloses a preparation method of rice bran nutrient. The rice bran nutrient extracted and separated by using the preparation method contains a plurality of nutrition constituents such as rice bran protein, rice bran polysaccharide, rice bran fiber, phytic acid, minerals and the like. The preparation method is simple and helpful for the industrial large-scale production. The preparation method disclosed by the invention comprises the following steps of: (1) cleaning, drying and crushing 1000 parts of rice bran, screening by using a 80-mesh sieve, carrying out muggy and high-temperature vapour treatment at 85 DEG C for 5-10 min, gradually heating up to 105 DEG C in 10-15 min, and carrying out heat preservation treatment for 10-15 min; (2) adding the materials obtained in the step (1) into petroleum ether with the weight of 2-3 times, stirring at 55-58 DEG C for 0.5-0.8 h, filtering and drying filter residue at 105 DEG C for 0.5-1.5 h; (3) mixing the materials obtained in the step (2) by using a hydrochloric acid water solution with the pH value of 5.0-5.5 in the material-liquid weight ratio of 1:(8-10), adding 15-25 parts of combined enzyme A, keeping at 38-45 DEG C, stirring and reacting for 1-2 h, carrying out centrifugalization at 3500-4000 r/min, and collecting supernate; (4) adding 15-20 parts of combined enzyme B into the supernate obtained in the step (3), keeping at 55-60 DEG C, stirring and reacting for 1-2 h, carrying out centrifugalization at 3500-4000 r/min, and collecting the supernate; and (5) carrying out vacuum concentration at 50-55 DEG C on the supernate obtained in the step (4), and carrying out freeze drying at subzero 20-25 DEG C to obtain the rice bran nutrient.

Description

[0001] technical field [0002] The invention belongs to the technical field of food processing and relates to a preparation method of rice bran nutrients. [0003] Background technique [0004] Rice bran is the husk of gramineous rice. It is a mixture of cortex, rice germ and a small amount of broken rice that are rolled down during rice milling. It accounts for about 5% to 6% of rice. It is not only rich in sources, but also comprehensive in nutrition. Rice bran is rich in unsaturated fatty acids, tocopherols, tocotrienols, lipopolysaccharides, edible fiber, squalene, γ-oryzanol and other physiologically active substances. These substances have significant functional effects on preventing human heart and cerebrovascular diseases, anti-cancer, enhancing immunity, lowering blood lipids, preventing constipation and obesity, and are important raw materials for health food, medicine, and chemical manufacturing industries, and are popular all over the world. [0005] to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/105A23L1/29A23L7/104A23L33/00
Inventor 张丽琍祁斌吴雷张超
Owner ANHUI YANZHIFANG FOOD
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