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Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds

A methicillin-resistant and peony seed extraction technology, which is applied in the directions of antibacterial drugs, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve problems such as no relevant reports, and achieve a simple operation process , improve economic benefits and medicinal value, improve the effect of medicinal value

Active Publication Date: 2012-02-29
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to previous research findings, peony seed oil mainly contains linolenic acid, oleic acid, linoleic acid, palmitic acid and stearic acid and other oil substances; the chemical components related to non-oil are oleanolic acid, hedera saponin, Kaempferol, luteolin, apigenin, coylin, stigmasterol and other substances, in addition, it is known that peony seeds have certain antibacterial effects on Bacillus subtilis, Salmonella, Rhizopus, Aspergillus niger and Barmonella, while antibacterial The activity of methicillin-resistant Staphylococcus aureus infection has not been reported

Method used

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  • Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds
  • Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds
  • Method for extracting medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds

Examples

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Effect test

Embodiment 1

[0030] The method for extracting anti-methicillin-resistant Staphylococcus aureus drug components from peony seeds, the specific steps are:

[0031] Step 1. Take 100 parts of peony seeds and 300 parts of ethanol solution with a concentration of 60% according to the weight fraction ratio, first crush the peony seeds to 200 mesh particles, add the ethanol solution to the peony seeds, mix well, and put them in the container. Carry out airtight soaking for 2 days, filter to obtain extract I and filter residue I, and set aside;

[0032] Step 2: Add 200 parts of ethanol solution with a concentration of 60% in the container containing filter residue I, mix well, carry out airtight soaking for 2 days, filter to obtain extract II and filter residue II, and extract extract II and extract II Mix to obtain a mixed solution for subsequent use;

[0033] Step 3, take 1 part of the mixed solution and 8 parts of water according to the weight fraction ratio, mix evenly, let stand for 2 hours, ...

Embodiment 2

[0039] The method for extracting anti-methicillin-resistant Staphylococcus aureus drug components from peony seeds, the specific steps are:

[0040] Step 1. Take 100 parts of peony seeds and 350 parts of ethanol solution with a concentration of 70% according to the weight fraction ratio, first crush the peony seeds to 300 mesh particles, add the ethanol solution to the peony seeds, mix well, and put them in the container. Carry out airtight soaking for 4 days, filter to obtain extract I and filter residue I, and set aside;

[0041] Step 2: Add 250 parts of ethanol solution with a concentration of 70% in the container containing filter residue I, mix well, carry out airtight soaking for 3 days, filter to obtain extract II and filter residue II, and extract extract II and extract II Mix to obtain a mixed solution for subsequent use;

[0042] Step 3, take 1 part of the mixed solution and 12 parts of water according to the weight fraction ratio, mix evenly, let stand for 3 hours,...

Embodiment 3

[0048] The method for extracting anti-methicillin-resistant Staphylococcus aureus drug components from peony seeds, the specific steps are:

[0049] Step 1. Take 100 parts of peony seeds and 400 parts of ethanol solution with a concentration of 80% according to the weight fraction ratio, first crush the peony seeds to 400 mesh particles, add the ethanol solution to the peony seeds, mix well, and put them in the container. Carry out airtight soaking for 5 days, filter to obtain extract I and filter residue I, and set aside;

[0050] Step 2: Add 300 parts of ethanol solution with a concentration of 80% in the container containing filter residue I, mix well, carry out airtight soaking for 3 days, filter to obtain extract II and filter residue II, and extract extract II and extract II Mix to obtain a mixed solution for subsequent use;

[0051] Step 3, take 1 part of the mixed solution and 16 parts of water according to the weight fraction ratio, mix evenly, let stand for 5 hours,...

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Abstract

The invention discloses a method for extracting a medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds, which comprises: mixing peony seeds and ethanol solution, and soaking the peony seeds under a closed condition for 2 to 5 days; obtaining extract I and filter residue I; soaking the filter residue I in ethanol again to obtain extract II; mixing the extract Iand the extract II, adding water into the mixed extract, mixing, standing and obtaining supernate; subjecting the obtained supernate to extraction by an ethyl acetate solvent twice; mixing the extract obtained by the two times of extraction; and adding saturated solution of n-butyl alcohol into the mixture for extraction twice, mixing the n-butyl alcohol extract obtained by the two times of extraction, standing the mixture for 2 to 5 days, and obtaining the product. The method for extracting the medicinal ingredient against methicillin-resistant staphylococcus aureus from peony seeds, which is provided by the invention, is simple in process and operation, the content of the medicinal ingredient against methicillin-resistant staphylococcus aureus, which is obtained from peony seeds, is high, the purity of the medicinal ingredient is high, and the antibacterial effect is obvious.

Description

technical field [0001] The invention relates to a method for extracting medicinal components, in particular to a method for extracting anti-methicillin-resistant Staphylococcus aureus medicinal components from peony seeds. Background technique [0002] With the widespread use of antibiotics, the variation of bacterial structure and morphology has caused more and more bacteria to develop resistance to antibiotics, and the degree of drug resistance is increasing day by day. At present, methicillin-resistant Staphylococcus aureus (MRSA) infection, hepatitis B virus HBV and AIDS are listed as the three most difficult infectious diseases in the world. Methicillin-resistant Staphylococcus aureus is resistant to a variety of antibiotics. Methicillin-resistant Staphylococcus aureus is one of the main pathogenic bacteria causing nosocomial clinical infections because of its strong pathogenicity and poor therapeutic effect. Although vancomycin can be used to treat infections caused b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P31/04A61K131/00
Inventor 尹卫平刘普李文渊李亮邓瑞雪
Owner HENAN UNIV OF SCI & TECH
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