Production method of single cell oil by culturing Cryptococcus aerius with utilization of corncob residue hydrolysate

A cryptococcal yeast and single-cell oil technology, which is applied in the field of cultivating cryptococcus yeast to ferment and produce single-cell oil by using corn cob xylose residue hydrolyzate, to achieve the effects of reducing production costs, ensuring sustainable energy supply, and alleviating energy crises

Inactive Publication Date: 2012-03-28
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieval, the use of xylose residue and delignified xylose residue hydrolyzate to cultivate oleaginous microorganisms to produce single-cell oil has not been reported. Therefore, the use of xylose residue and delignified xylose residue to ferment and produce single-cell oil The method has extremely important practical significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of cellulase solution: inoculate Penicillium decumbens JU-A10 in a regular amount in the enzyme-producing medium, shake culture at 250 rpm at 30°C for 96 h to produce enzyme, centrifuge at 6000 rpm for 30 min, and collect The supernatant is the cellulase crude enzyme liquid;

[0022] The formula of the above-mentioned enzyme production medium is: 20g / l xylose residue, 30g / l bran, 6g / l microcrystalline cellulose, 5g / l bean cake powder, 2g / l ammonium sulfate, 1g / l urea, 2g / l nitric acid Sodium, 3g / l potassium dihydrogen phosphate, 0.5g / l magnesium sulfate, 3ml / l Tween 80;

[0023] (2) Preparation of saccharification solution: take delignified xylose residue, add cellulase solution with 25 FPA per gram of dry substrate, and saccharify at 45°C for 72 hours; collect the saccharification solution and put the residual cellulose in a water bath at 80°C for 30 minutes. Inactivate the enzyme, then centrifuge the saccharification solution at 8000 rpm for 10 minute...

Embodiment 2

[0029] (1) Preparation of cellulase solution: Inoculate Penicillium decumbens in an enzyme-producing medium in a conventional amount, culture at 30°C and shake at 250rpm for 84h to produce enzyme, centrifuge at 6000rpm for 30min, and collect the supernatant as cellulose Enzyme crude enzyme solution;

[0030] The formula of the above-mentioned enzyme production medium is: 20g / l xylose residue, 30g / l bran, 6g / l microcrystalline cellulose, 5g / l bean cake powder, 2g / l ammonium sulfate, 1g / l urea, 2g / l nitric acid Sodium, 3g / l potassium dihydrogen phosphate, 0.5g / l magnesium sulfate, 3ml / l Tween 80;

[0031] (2) Preparation of saccharification solution: take delignified xylose residue, add cellulase solution with 30 FPA per gram of dry substrate, and saccharify at 45°C for 72 hours; collect the saccharification solution and place it in a water bath at 80°C for 30 minutes to remove residual cellulose Inactivate the enzyme, then centrifuge the saccharification solution at 9000 rpm f...

Embodiment 3

[0037] (1) Preparation of saccharification solution: take delignified xylose residue, add commercial cellulase solution (from Qingdao Kangdien Biotechnology Co., Ltd.) with 25 FPA per gram of dry substrate, and the buffer system is pH4.8 The 0.05M HAC-NaAc buffer solution was saccharified at 45°C for 72 hours; the saccharification solution was collected and placed in a water bath at 80°C for 30 minutes to inactivate residual cellulase, and then the saccharification solution was centrifuged at 8000rpm for 10 minutes to obtain a clear saccharification solution;

[0038] (2) Detoxification treatment of the saccharified liquid: heating the saccharified liquid in a water bath to 80° C., adding 1.0% activated carbon by mass percentage, stirring for 30 minutes, and then vacuum filtering to remove the activated carbon to obtain a detoxified saccharified liquid;

[0039] (3) Utilize saccharification liquid to ferment Cryptococcus yeast: Prepare oil-producing saccharification liquid ferm...

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PUM

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Abstract

The invention discloses a production method of single cell oil by culturing Cryptococcus aerius with the utilization of corncob residue hydrolysate. In comparison with the prior art, the method for producing the single cell oil by inorganic acid or cellulase hydrolysate of corn straws has the characteristics of convenient technology, low cost, no pollution and high grease output, remarkably overcomes environmental pollution caused by chemical reagents such as a lot of dilute acid required for obtaining the hydrolysate needed in the above experiment, and simultaneously is used to greatly reduce the production cost. According to the invention, a lot of biomass resources are utilized and agricultural waste materials are processed. Therefore, the method provided by the invention is of great practical significance and has important economical values for improving the energy shortage state of our country, guaranteeing sustainable supply of energy in our country and alleviating energy crisis.

Description

technical field [0001] The invention relates to a method for producing single-cell oil, in particular to a method for cultivating Cryptococcus curvatus by using corncob xylose residue hydrolyzate (saccharification liquid) to ferment and produce single-cell oil. Background technique [0002] Single-cell oils, also known as microbial oils, are oils produced by microorganisms or algae using carbohydrates, hydrocarbons and ordinary oils as carbon sources, nitrogen sources, supplemented by inorganic salts, and some oils have high added value. Its fatty acid composition is similar to that of vegetable oils, mainly C16 and C18 fatty acids, such as palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2 ), linolenic acid (C18:3), etc. Microbial oil can be widely used in food, medicine, fine chemical industry and other industries. [0003] At present, industrial oils and edible oils at home and abroad mainly come from animals, plants and fossil resourc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 宋欣马晓静
Owner SHANDONG UNIV
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