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Nanoparticle preparation method and nanoparticles prepared by method thereof

A nanoparticle and microemulsion technology, which is applied in the direction of medical preparations of non-active ingredients, pharmaceutical formulas, inorganic non-effective ingredients, etc., can solve the problems of poor repeatability of gene transfection and achieve the effect of ensuring safety

Active Publication Date: 2013-06-19
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when calcium phosphate nanoparticles are used as gene or vaccine vectors, the reproducibility of gene transfection is poor

Method used

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  • Nanoparticle preparation method and nanoparticles prepared by method thereof
  • Nanoparticle preparation method and nanoparticles prepared by method thereof
  • Nanoparticle preparation method and nanoparticles prepared by method thereof

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preparation example Construction

[0019] The invention provides a method for preparing nanoparticles, the method comprising mixing a first microemulsion with a second microemulsion to obtain a nanoparticle a; the first microemulsion contains a surfactant, an organic solvent and a first reactant An aqueous solution, the second microemulsion contains an aqueous solution of a surfactant, an organic solvent and a second reactant; the first reactant and the second reactant can form a precipitate after contact; the surface activity in the first microemulsion The agent is the same as the surfactant in the second microemulsion.

[0020] In order to realize the controllable synthesis of nanoparticles and prevent their agglomeration, the surfactant uses oil-soluble polyoxyethylene-polyoxypropylene block copolymer surfactant (Pluronic) and water-soluble polyoxyethylene-polyoxypropylene block copolymer surfactant. A mixture of segment copolymer surfactants (Pluronic), cationic surfactants such as cetyltrimethylammonium br...

Embodiment 1

[0044] Preparation of nanoparticles

[0045] (1) Preparation of the first microemulsion: after getting 3.3g Pluronic L64 (Germany BASF Company), 0.3g Pluronic L61 (Germany BASF Company) and 0.4g isopropyl myristate (U.S. ACROS Company) to mix well, add dropwise 45μl calcium chloride aqueous solution (12.5mM), stirred for 12h.

[0046] (2) Preparation of the second microemulsion: after getting 3.3g Pluronic L64 (Germany BASF Company), 0.3g Pluronic L61 (Germany BASF Company) and 0.4g isopropyl myristate (U.S. ACROS Company) to mix well, add dropwise 45μl disodium hydrogen phosphate aqueous solution (12.5mM), stirred for 12h.

[0047] (3) At 25°C, add the second microemulsion to the first microemulsion, and continue stirring for 12 hours; centrifuge at 8000 rpm for 30 minutes, wash twice with absolute ethanol, and finally disperse with 1ml double distilled water to obtain nanoparticle sol.

[0048] The obtained nanoparticles were detected by a laser particle size analyzer, an...

Embodiment 2

[0050] Preparation of nanoparticles

[0051] (1) Preparation of the first microemulsion: after getting 2.14g Pluronic P123 (Germany BASF Company), 1.29g Pluronic P85 (Germany BASF Company) and 0.57g n-hexane and mix well, add dropwise 135 μ l calcium chloride aqueous solution (0.1M) , stirred for 12h.

[0052] (2) Preparation of the second microemulsion: After mixing 2.14g Pluronic P123 (Germany BASF Company), 1.29g Pluronic P85 (Germany BASF Company) and 0.57g n-hexane, drop 135 μl of diammonium hydrogen phosphate aqueous solution (0.06M ), stirred for 12h.

[0053] (3) At 25°C, add the second microemulsion to the first microemulsion, and continue to stir for 12 hours; centrifuge at 8000 rpm for 30 minutes, wash twice with absolute ethanol, and finally disperse with 1ml of double-distilled water to obtain nano Particle sol.

[0054] The obtained nanoparticles were detected by a laser particle size analyzer, and the result was similar to that of Example 1. The surface of th...

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Abstract

The invention provides a nanoparticle preparation method. According to the preparation method, a first microemulsion is mixed with a second microemulsion. The first microemulsion contains a surfactant, an organic solvent and a first reactant aqueous solution. The second microemulsion contains a surfactant, an organic solvent and a second reactant aqueous solution. Precipitates can be formed afterthe first reactant contacts with the second reactant. The surfactant in the first microemulsion is the same as that in the second microemulsion, and the surfactant is a mixture of an oil-soluble polyoxyethylene-polyoxypropylene segmented copolymer surfactant and a water-soluble polyoxyethylene-polyoxypropylene segmented copolymer surfactant or a cationic surfactant of cetyl trimethyl ammonium bromide and the like. The nanoparticles provided by the invention have good safety and can obviously raise gene transfection level and promote proteins or drugs to enter into cells.

Description

technical field [0001] The invention relates to a method for preparing nanoparticles and nanoparticles prepared by the method. Background technique [0002] Organisms are complex organisms, and their natural defense system plays an extremely important role in maintaining the dynamic stability of the organism. Reticuloendothelial system, monocyte-macrophage system and immune system all play an important role. However, while protecting the body, some drug molecules for treating diseases may be cleared or degraded before reaching the target organ due to these protective mechanisms of the body, making it difficult to target organs or achieve effective accumulation at the target site. Therefore, in the process of disease treatment, the stability of the drug in the body, the circulation time or half-life, and whether it can quickly reach the target organ and form an effective accumulation play a key role in the rapid and effective cure of the disease. [0003] Based on the poten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/48A61K47/34A61K47/02A61K47/42
Inventor 陈春英许利耕
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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