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Compounds extracted from Chinese herbal medicine semen cassiae and their application

A technology for compounds and uses, which is applied in the field of extracting and separating compounds with pharmacological activity from Chinese medicine cassia seeds, can solve the problems such as insufficient depth of traditional Chinese medicine cassia seeds.

Inactive Publication Date: 2012-05-02
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the chemical constituents of the traditional Chinese medicine Semen Cassiae is not deep enough, and it is necessary to further explore its active ingredients and clarify the relationship between its pharmacological activity and active ingredients

Method used

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  • Compounds extracted from Chinese herbal medicine semen cassiae and their application
  • Compounds extracted from Chinese herbal medicine semen cassiae and their application
  • Compounds extracted from Chinese herbal medicine semen cassiae and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The extraction and separation method of embodiment 1 compound I and II:

[0026] 20 kg of dried cassia seeds, crushed, extracted by percolation with 95% alcohol of 10 times the amount of crude drug, concentrated under reduced pressure until no alcohol smell, added appropriate amount of water to disperse, extracted with petroleum ether, chloroform, n-butanol in sequence, recovered solvent to obtain petroleum ether Extract 100 g, chloroform extract 150 g, n-BuOH extract 200 g and the remaining water fraction. The n-BuOH extract was separated by silica gel (100-200 mesh) column chromatography, chloroform-methanol gradient elution, every 500ml fraction was collected, and the solvent was recovered, wherein the 88th-90th fraction was filtered to remove the methanol-insoluble fraction, The dried powder is compound II, where fractions 109-113 are combined, separated by silica gel (100-200 mesh) column chromatography, eluted with ethyl acetate-methanol-water (10:2:1), and collec...

Embodiment 2

[0027] The activity of embodiment 2 naphthalene compounds I in protecting the liver

[0028] 1 Experimental materials

[0029] 1.1 Samples and reagents

[0030] Carbon tetrachloride was provided by Beijing Chemical Reagent Factory. Alanine aminotransferase (ALT) and aspartate transferase (AST) detection kits were provided by Nanjing Jiancheng Institute of Biology. Positive control drug: bifendate, Dezhou Deyao Pharmaceutical Co., Ltd., batch number 040410. Test sample: Naphthalene compounds isolated above I: 1-hydroxyl-2-acetyl-3,8-dimethoxy-6-O-[β-D-apiofuranosyl-(1→2)-β-D-glucopyranosyl]- naphthalene.

[0031] 1.2 Animals

[0032] Kunming mice, male, weighing 18-22 g, were provided by the Experimental Animal Center of China Academy of Chinese Medical Sciences.

[0033] 1.3 Instruments

[0034] 752 ultraviolet grating spectrophotometer, Shanghai Precision Scientific Instrument Co., Ltd.; LDZ5-2 centrifuge, Beijing Medical Centrifuge Factory; DKB-8A electric heating con...

Embodiment 3

[0043] The activity of embodiment 3 anthraquinone compounds II in antioxidation

[0044] 1 Experimental materials

[0045] 1.1 Samples and reagents

[0046] DPPH (1,1-diphenyl-2-pichydrazyl free radical), product of Sigma Company. Positive control drug vitamin C, Sigma company. Test sample: the anthraquinone compound II isolated above, namely 1-demethylaurantio-obtusin-2-O-β-D-glucopyranoside

[0047] 1.2 Instruments

[0048] UV-T6 new century ultraviolet spectrophotometer, KQ-500E medical ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), Sartorius 1 / 10,000 electronic balance.

[0049] 2 Experimental content

[0050] 2.1 Detection method for scavenging DPPH free radicals

[0051] Accurately weigh 2.1mg VC and 2.4mg of compound II respectively, dissolve them in 10mL measuring bottles and prepare them with ethanol to make the concentrations respectively 0.21mg / mL and 0.24mg / mL, and then divide the above liquids to be tested by 2 times, 6 times, 18 times, 54 time...

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Abstract

The invention discloses structures of two new compounds deriving from Chinese herbal medicine semen cassiae, and the two compounds are 1-hydroxyl-2-acetyl-3, 8-dimethoxy-6-O-[beta-D-apiofuranosyl-(1-2)-beta-D-glucopyranosyl]-naphthalene (I), and 1-demethylaurantio-obtusin-2-O-beta-D-glucopyranoside (II) respectively. Simultaneously, the invention also discloses an extraction and separation method of the two new compounds, as well as application of their derivatives in preparing active medicaments for antioxidation and liver protection.

Description

technical field [0001] The invention belongs to the field of phytochemistry, specifically belongs to the field of extraction, separation and preparation of active ingredients of traditional Chinese medicines, and in particular relates to the extraction and separation of compounds with pharmacological activity from the seeds of cassia cassia and a method thereof. Background technique [0002] Semen Cassiae (Semen Cassiae) is the dry mature seed of Cassia obtusifo L. or Cassia tora L., a leguminous plant. The chemical composition of cassia seeds is mainly anthraquinones, in addition to benzopyrone, sterols, fatty acids and other components. Modern pharmacological studies have shown that Semen Cassiae has the activities of lowering blood pressure, lowering blood fat, protecting liver and inhibiting bacteria, and is widely used. In addition, cassia seed is a traditional Chinese medicine that can be used as both medicine and food, and is a good raw material in the field of healt...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P39/06
Inventor 王祝举唐力英吴宏伟
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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