Triticum aestivum farnesyl phosphate synthase (TaFPS) gene as well as isolation colonizing and enzyme activity measuring method thereof

Abstract

The invention belongs to molecular biology and relates to a technology for isolation colonizing of a triticum aestivum farnesyl phosphate synthase (TaFPS) gene as a key enzyme gene participating in isoprene-like substances such as wheat chlorophyll, carotenoid and the like as well as prokaryotic expression of enzyme protein, separated purification of the enzyme protein and detection in vitro of the enzyme activity. The invention provides important technical storage for further constructing a eukaryotic gene expression vector of the TaFPS gene and converting the eukaryotic gene expression vector into a corresponding crop, particularly obtaining plants with important commercial value by means of secondary metabolism, discussing the relationships between the overexpression of the TaFPS gene in a receptor plant and important economical characters such as a secondary metabolite, the seed size of crops, the grain weight and the like, further improving the yield of the crops, analyzing the structures of an introne, an exon and a promoter of the TaFPS gene, researching the functions of the promoter and developing a relevant molecular marker.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to the isolation and cloning of a key enzyme gene farnesyl pyrophosphate synthase gene TaFPS involved in the synthesis of isoprenoid substances such as wheat chlorophyll and carotenoids, the prokaryotic expression of enzyme protein, and the enzyme Protein separation and purification and enzyme activity in vitro detection technology. Background technique [0002] Isoprenoid substances exist in all organisms, especially in plants, and are important organic substances necessary for maintaining plant growth and development, photosynthesis, etc. At present, more than 30,000 plant isoprenoids have been discovered, and this number is still increasing year by year. Such substances have various roles in organisms, and can be used as photosynthetic pigments (such as chlorophyll, carotenoids), growth substances and plant hormones (cytokinins, abscisic acid, gibberellins and brassinosteroids), ...

AI Technical Summary

Problems solved by technology

So far, farnesyl pyrophosphate synthase genes have been isolated and cloned from 40 plant species including Arabidopsis thaliana, rice (Oryza sativa), corn (Zea mays), sorghum (Sorghum bicolor), and rubber (Hevea brasiliensis). , before the application of the present invention, there has not been disclosed or published about the separation and cloning of the farnesyl pyrophosphate synthase gene TaFPS from wheat (Triticum aestivum), the prokaryotic gene expression of encoding protease, the separation and purification of enzyme protein and its enzyme activity in vitro Research data information such as detection and kinetic parameters

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