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Method for extracting paeoniflorin from radix paeoniae lactiflorae

A technology of paeoniflorin and white peony, which is applied in the field of extracting high-content paeoniflorin from white peony, can solve the problems of small preparation amount, poor process reproducibility, low yield of high-content paeoniflorin, etc., and achieve fast column passing speed, The effect of less impurities and shorter time

Inactive Publication Date: 2012-05-09
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the existing process has the problems of low yield, small amount of preparation, or poor process reproducibility in the preparation of high-content paeoniflorin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Crush the white peony, take 5kg, put it into an ultrasonic extraction tank, configure 60% ethanol solution hydrochloric acid to adjust the pH of the solution, add 50L ultrasonic extraction (power 2kw) for 1 hour, extract twice, combine the two extracts to adjust with sodium hydroxide solution After neutral filtration, the ethanol was recovered under reduced pressure to obtain the concentrated solution, which was dispersed in water, and then adsorbed by adding 5L NKA-9 macroporous resin at a flow rate of 5kg / h. The adsorption was completed. The water washing sugar reaction was negative, and then 25L 70% pH3 ethanol solution was eluted. Adjust the eluent to neutral to recover ethanol, then add 3LHPD-100 macroporous resin adsorption column for adsorption, flow rate 1.5kg / h, 18L40% methanol elution, collect eluent, eluent add 2L acidic alumina short column, collect Lower the column liquid, reduce the pressure back to ethanol to 1L, add 3L ethyl acetate n-butanol (3:1) mixed s...

Embodiment 2

[0026] Crush the white peony, take 5kg, put it into an ultrasonic extraction tank, configure 70% methanol solution sulfuric acid to adjust the pH of the solution, add 25L ultrasonic extraction (power 2kw) for 0.5 hours, extract 3 times, combine the two extracts to adjust with potassium hydroxide solution After neutral filtration, the ethanol was recovered under reduced pressure to obtain the concentrated solution, which was dispersed with water, and 5L NKA-2 macroporous resin was added for adsorption. The flow rate was 5kg / h. The adsorption was completed. The water washing sugar reaction was negative. Then 40L 40% pH2 ethanol was eluted and washed. Recover ethanol by deliquation and neutralization, then add 4L X-5 macroporous resin adsorption column for adsorption, flow rate 2kg / h, 28L methanol elution, collect eluate, add eluate to 3L acidic alumina short column, collect lower column Return the ethanol to 2L under reduced pressure, add 2L ethyl acetate n-butanol (3:2) mixed sol...

Embodiment 3

[0028] Crush the white peony, take 5kg, put it into an ultrasonic extraction tank, configure 50% acetone solution hydrochloric acid to adjust the pH of the solution, add 40L ultrasonic extraction (power 3kw) for 1 hour, extract twice, combine the two extracts to adjust with sodium hydroxide solution After neutral filtration, the ethanol was recovered under reduced pressure to obtain the concentrated solution, which was dispersed in water, and 5L LSA-21 macroporous resin was added for adsorption. The flow rate was 5kg / h. The adsorption was over. The water washing sugar reaction was negative. Then 30L 60% pH1 ethanol was eluted and washed. The ethanol is recovered by deliquation and neutralization, and then added to 3L D-101 macroporous resin adsorption column for adsorption, flow rate 2kg / h, 18L 40% for elution, collect the eluate, add the eluate to a 3L acidic alumina short column, and collect Return the column liquid to 1.5L of ethanol under reduced pressure, add 2L of ethyl ac...

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Abstract

The invention relates to a method for extracting paeoniflorin from radix paeoniae lactiflorae. The process method comprises the following steps: crushing medicinal radix paeoniae lactiflorae materials; carrying out ultrasonic extraction with an acidic alcohol or ketone solution; adding polar macroporous resin into the concentrated solution for adsorption; eluting with 40-70% acidic ethanol; recovering ethanol in the eluent; adding nonpolar macroporous resin for adsorption; eluting with 30-50% methanol; allowing the eluent to pass through an acidic aluminum oxide short column; concentrating, adding a mixed solution of ethyl acetate and n-butyl alcohol, and extracting for 3-5 times; and carrying out vacuum drying to obtain the product. The method for producing paeoniflorin has the advantages of high yield and high content, thereby being applicable to industrial preparation.

Description

Technical field: [0001] The invention relates to a method for extracting paeoniflorin from white peony, in particular to a method for extracting high-content paeoniflorin from white peony. Background technique: [0002] Paeoniflorin is a hygroscopic amorphous white powder. The tetraacetate is a colorless needle crystal. Melting point: 196°C. Paeoniflorin is stable in an acidic environment (pH 2~6) and is easily decomposed by heat. [0003] Paeoniflorin has significant analgesic, sedative and anticonvulsant effects. It can also dilate the coronary arteries, increase coronary flow, combat acute myocardial ischemia, lower blood pressure, and also have liver protection, anti-tumor, and improve human immunity. It is clinically used in the treatment of coronary heart disease; it can be used as an auxiliary drug in the treatment of senile diseases, strengthening physical fitness and immune function, anti-inflammatory and relieving cough, expectorating and relieving asthma, etc., especial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 李法庆杨成东刘东锋
Owner 苏州宝泽堂医药科技有限公司