Aqueous solution of silk fibroin and preparation method for aqueous solution

A technology of silk fibroin and aqueous solution, which is applied in the field of biomedicine, can solve problems such as difficulty in applying silk fibroin, and achieve the effect of avoiding waste

Active Publication Date: 2012-06-27
JIANGSU HUAJIA SILK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aloe vera gel is sterilized at a high temperature of 120°C for 3-5 seconds, and then filtered after rapid cooling after sterilization. However, it needs to be sterilized by filtration, which is difficult to apply to silk fibroin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Put 100 g of silkworm raw silk into 5 L of 0.05% Na 2 CO 3 In aqueous solution, at 98-100 o C treatment for 30 minutes, repeated 3 times to degumming silk, fully washed and dried to obtain pure silk fiber. Add pure silk fiber to the ternary solution (by CaCl 2 / water / ethanol molar ratio is 1:8:2) solution, at 70 o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag and dialyze with deionized water for 4 days to remove CaCl 2、 Ethanol and other impurities are removed to obtain a pure silk fibroin solution.

[0018] The pure silk fibroin solution was centrifuged for 30 minutes in a centrifuge with a centrifugal speed of 4000 rpm to remove impurities produced during the dissolution and dialysis process.

[0019] Add 5% glycerin and 0.01% potassium chloride to the silk fibroin solution; put it into a high-temperature-resistant container, and sterilize it in a high-temperature steam sterilize...

Embodiment 2

[0021] Put 0.1 kg of raw silk into 3 liters of sodium carbonate aqueous solution with a mass concentration of 0.15%, treat it at 98-100° C. for 2 hours, degumming the raw silk, and obtain pure silk fiber after fully washing. The dried pure silk fiber is heated and dissolved at 60±5° C. with 1 liter of lithium bromide solution with a concentration of 9.0 mol / liter to obtain a silk fibroin mixed solution. The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water for 3 days to remove impurities such as lithium bromide to obtain a pure silk fibroin solution.

[0022] The pure silk fibroin solution was centrifuged for 20 minutes in a centrifuge with a centrifugal speed of 5000 rpm to remove impurities produced during the dissolution and dialysis process.

[0023] Add 1% sorbitol of silk fibroin to the silk fibroin solution, add 0.001% of silk fibroin sodium thiocyanate; put it into a high-temperature resista...

Embodiment 3

[0025] Put 200 grams of waste silk into 8 liters of sodium carbonate aqueous solution with a mass concentration of 0.05%, degumming at 98-100° C. for 0.5 hour, repeat the treatment 3 times, and obtain pure silk fibroin after fully washing. The dried pure silk fibroin is heated and dissolved in 1 liter of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 at 75±5°C to obtain a silk fibroin mixed solution. The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water for 3 days to obtain a pure silk fibroin solution.

[0026] The pure silk fibroin solution is centrifuged in a centrifuge with a centrifugal speed of 10,000 rpm for 10 minutes to remove impurities generated during the dissolution and dialysis process.

[0027] Add 2% mannitol of silk fibroin to the silk fibroin solution, add 0.02% potassium bromide of silk fibroin; put it in a high-temperature resistant container, and put it...

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PUM

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Abstract

The invention discloses an aqueous solution of silk fibroin and a preparation method for the aqueous solution, and belongs to the technical field of biomedicine. According to the technical scheme, the method comprises the following steps of: adding strongly polar polyol or soluble salt for hindering gelatinization of a silk fibroin solution into the silk fibroin solution, performing high-temperature steam sterilization, and thus obtaining the aqueous solution of the silk fibroin, wherein the aqueous solution of the silk fibroin can be stored for over 3 months at room temperature without gelatinization or deterioration and has stability. The aqueous solution of the silk fibroin is suitable to be prepared into various silk fibroin films, hydrogel, porous materials, microspheres and the like used for cosmetics, skin-care products, sun lotion, tissue engineering materials, medicament carriers, artificial skin and the like. Because the stable aqueous solution of the silk fibroin can be stored for long time, the preparation-and-use workshop type production mode of a silk fibroin biomaterial can be changed into a large-scale industrialized production mode.

Description

technical field [0001] The invention relates to a protein solution and a preparation method thereof, in particular to a stable protein aqueous solution prepared by using silk fibroin as a raw material and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Silk protein is a high-purity protein secreted by endothelial cells on the inner wall of silk glands of silkworm silk. It is composed of 20 kinds of amino acids such as tyrosine, alanine, and serine. Biodegradable. In the field of non-textiles, silkworm silk fibers have long been used as surgical sutures. In recent years, a large number of research results on the application of silk fibroin in artificial skin, artificial blood vessels, artificial bones, drug slow-release carriers and enzyme-immobilized materials have shown that silk fibroin materials are non-toxic, non-irritating, and non-immunogenic. biomaterials with broad application prospects. The production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08K13/02C08K5/053C08K3/16C08K5/372A61K8/64A61K47/42A61Q17/04A61Q19/00A61Q1/00A61L27/22A61L27/60A61L27/50
Inventor 卢神州吴锡龙李明忠
Owner JIANGSU HUAJIA SILK
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