Aging oil biodesulfurization microbial agent and method for treatment of aging oil with microbial agent
A technology for biological desulfurization and aging of oil, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] The water sample that will be taken from in the fish culture landscape pond is brought back to the laboratory and is used in aseptic room with 400mL enriched culture solution (in the purple sulfur bacteria seed culture medium, adding mass concentration is 2% Na 2 S·9H 2 O is the enrichment medium) into a 500mL Erlenmeyer flask, pour the collected water sample to the mouth of the bottle, airtight, oscillate and mix, and cultivate in a 37°C, 3000-5000lx light incubator for 10 days until cultured When the substance turns red, use a pipette gun to transfer 1mL of the enriched culture to another Erlenmeyer flask filled with fresh enriched culture solution. Repeat the above operation 2 to 3 times to obtain well-growing light and bacterial enrichment. Cultures. Then the enriched culture was diluted in a gradient, and then separated from the solid medium with agar added, and the colony was picked and cultured in the liquid medium (i.e. seed medium) for 7 days, and then repeate...
Embodiment 2
[0052] Soil samples collected from acidic (pH about 2 to 3) and moist soil (the sampling soil layer is 15cm away from the ground) were added to the Thiobacillus denitrifica seed medium in batches in a natural state, and the cultured soil samples were taken out after 3 weeks for separation nourish. Weigh 0.5g of the cultured soil sample, quickly pour it into a sterile water bottle with glass beads (the amount of glass beads should fill the bottom of the bottle is the best), shake for 5-10 minutes to fully break up the soil sample, which becomes 10 -2 soil suspension; use a sterile pipette to aspirate 10 -2 0.5mL of the soil suspension, put into 4.5mL sterile water and serve 10 -3 Dilution, so repeated, can make 10 -3 ~10 -8 After cultivation, take the turbid bacterial solution at the last level, dilute it in a gradient, and then separate it from the solid medium with agar added, pick but the colonies are cultured in the liquid medium for 7 days, and repeat the solid medium ...
Embodiment 3
[0055] The composition of the seed culture medium of purple sulfur bacteria PSE with the preservation number CCTCC NO: M2011276 is CH 3 COONa 3g / L, (NH 4 ) 2 SO 4 1.0g / L, MgSO 4 0.2g / L, NaCl 1g / L, KH 2 PO 4 0.3g / L, K 2 HPO 4 0.5g / L, CaCl 2 0.05g / L, yeast extract 0.1g, V B1 1Ml, 1L of distilled water, the isolated purple sulfur bacterium PSE with the preservation number CCTCC NO: M2011276 is cultured in the seed medium for 7 days under light, and the seed liquid of the bacteria can be obtained.
[0056] The composition of the seed culture medium of Thiobacillus denitrificans with the preservation number CCTCC NO: M2011135 is Na 2 HPO 4 1.2g / L,KH 2 PO 4 1.8g / L, NH 4 Cl 0.5g / L, KNO 3 5g / L, Na 2 S 2 o 3 10g / L, NaHCO 3 1g / L, MgSO 4 ·7H 2 O, CaCl 2 , MnSO 4 , FeCl 3 A small amount, 1L of distilled water, and the isolated Thiobacillus denitrificans with the preservation number CCTCC NO: M2011135 are placed in an oven at about 30°C for more than 7 day...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap