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Streptomyces strain and application thereof

A technology of streptomyces and strains, applied in the field of bioengineering, can solve the problems of weak activity in vivo, few development prospects, and narrow antifungal spectrum

Active Publication Date: 2012-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Clinically, with the rise of the fungal infection rate, the utilization rate of antifungal drugs continues to increase, followed by the continuous increase of drug-resistant strains, the curative effect of existing antifungal antibiotics is greatly reduced, and it is also difficult for clinical treatment. Therefore, it is more and more people's attention to find new antifungal antibiotics that are safe, efficient, low-toxicity and broad-spectrum.
[0005] In the past 10 years (1996-2005), about 174 new antifungal antibiotics have been reported in various countries, with more than 30 types of chemical structures. The fungal spectrum is too narrow, the activity in the body is weak, or because of the strong cytotoxicity, etc., the natural products themselves and the products with structural modifications have little development prospects

Method used

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  • Streptomyces strain and application thereof
  • Streptomyces strain and application thereof
  • Streptomyces strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Bacterial Strain Isolation and Purification

[0025] Soil samples were cross-sampled at five points in the area delineated in the East China Sea of ​​Zhejiang Province. Take 10g of soil samples at each point and put them into conical flasks. bottle, placed on a shaker and vibrated for 30 minutes, so that the bacteria in the soil sample were fully dispersed in the water, that is, 10 -1 bacteria liquid. After the inoculation loop was flame sterilized and cooled, dip a ring to dilute it to 10 -1 Bacterial solution was streaked and inoculated in four zones on Gao's No. 1 medium plate, cultured at 28°C for 5 days, and then single colonies were picked and streaked for further purification.

[0026] Take out the cultured single colony together with the small pieces of agar around it with a perforator, transfer it into a medium-free plate, culture it at 28°C for 4 days, and then transfer it into a plate coated with Candida albicans. The distance between the centers ...

Embodiment 2

[0028] Example 2 strain identification

[0029] (1) Morphological observation

[0030] It grows well on Gao's No. 1 medium. The color of the colony is gray, the shape of the colony is relatively round, the surface is wrinkled, the middle protrusion is white, and the diameter is medium-sized (about 5mm). Peak, 7d is fully mature. After culturing on the plate for 4 days, pick a single colony and observe it under an optical microscope. The results are as follows: figure 1 As shown, according to the morphological observation and the observation of spores and mycelium under the optical microscope, it can be initially identified as an actinomycete.

[0031] (2) 16S rDNA sequence analysis

[0032] Take 1 ring of activated actinomycetes strains, insert them into the seed medium and cultivate them for 24 hours, then insert them into the fermentation medium according to the inoculation amount of 10% (v / v), cultivate them at 200 r / min for 4 days at 28°C, and use Filter through gauze,...

Embodiment 3

[0050] The mensuration of embodiment 3 bacterial strain antibacterial activity

[0051] The antibacterial activity of Streptomyces ZJU088 against the tested strains was tested by the tube-and-dish method, and a positive control of mycocin and a blank control of normal saline were set up.

[0052] Take 1 ring of the above-mentioned activated strains and put them into a seed bottle, put 25ml of seed culture medium in a 250ml Erlenmeyer flask, the initial pH value is 7.0, and cultivate at 200r / min at 28°C for 24h to obtain a seed solution; Inoculate 10% (v / v) into a 250ml Erlenmeyer flask (containing 40ml fermentation medium), cultivate at pH 7.0, 200r / min at 28°C for 4 days, centrifuge the fermentation broth at 5000rpm for 15min, and leave the supernatant The antibacterial effect of the fermented liquid on Candida albicans was measured by the tube-and-plate method, and the results showed that it had a strong inhibitory effect on Candida albicans, and the diameter of the inhibiti...

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Abstract

The invention discloses a Streptomyces strain and an application thereof, belonging to the technical field of bioengineering. The strain is named as Streptomyces sp. ZJU088, and preserved in CGMCC (China General Microbiological Culture Collection Center) on May 19th, 2011, with preservation number CGMCC No.4889. The Streptomyces strain disclosed by the invention has a perfect effect in inhibitingfungi, particularly has a strong inhibition effect on tritirachium album, and is important in treating fungal diseases.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a Streptomyces strain and application thereof. Background technique [0002] Infections caused by fungi are divided into superficial fungal infections and deep fungal infections. The contagiousness of superficial fungal infection is strong, and its common pathogen is various tinea fungi, mainly invades skin, hair, nail (toe) etc., although these fungal diseases are stubborn but not fatal. The common pathogens of deep fungal infection are Candida and Aspergillus, which mainly invade internal organs and deep tissues, which are extremely harmful and often life-threatening. Among them, Candida albicans is a major type of deep fungal infection. It is the main cause of nosocomial blood infection, and it is highly lethal when infected in wounds and body fluids, and clinical studies have found that its fatality rate does not decrease with the use of conventional antifungal drugs....

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/10C12R1/465A61K35/741
Inventor 吴绵斌刘超超
Owner ZHEJIANG UNIV
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