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Preparation method of fibroins of different forms

A technology of silk fibroin and morphology, which is applied in the field of preparation of silk fibroin of different shapes, to achieve the effect of unique physical and chemical properties and low cost

Active Publication Date: 2012-07-11
THE HONG KONG POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing published technology is limited to the preparation of micro-sized silk fibroin powder

Method used

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  • Preparation method of fibroins of different forms
  • Preparation method of fibroins of different forms
  • Preparation method of fibroins of different forms

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preparation example Construction

[0050] figure 1 It is a flowchart of the preparation method of different forms of silk fibroin of the present invention. From figure 1 As can be seen, the preparation method of the present invention mainly comprises the following steps:

[0051] 1. Degumming of silk: use high temperature and high pressure water or boiled Na 2 CO 3 solution treatment to remove the sericin part of the cocoon silk material, then rinse with deionized water and then dry to obtain the degummed dry cocoon silk material. The cocoon silk material referred to here may include one or more of cocoon silk, spun silk and raw silk. In addition, high-temperature and high-pressure water refers to water having a temperature of 100 to 130° C. and a pressure of 0 to 0.18 MPa. It should be noted that all numerical ranges in which the lower limit is 0 herein do not include the lower limit of 0, but include the corresponding upper limit.

[0052] 2. Preparation of dried silk fibroin: dissolve the degummed dry ...

Embodiment 1

[0060] Put 100g of cocoon silk into deionized water, and then react in a steam heater at 120°C and 0.1MPa for 60min to remove the sericin on the surface. The degumming rate was measured by biuret reaction and weighing method, and the results confirmed that the silk surface sericin had been completely removed. Subsequently, the obtained degummed cocoon silk was rinsed with deionized water and dried for the next step.

[0061] Put 15g of dried degummed cocoon silk into 1000ml CaCl at 70°C 2 :CH 3 CH 2 OH:H 2 O=1:2:8 (mol) ternary solvent reacted for 6h to obtain silk fibroin solution. Then use a semi-permeable membrane to remove salt and other impurities to obtain a pure silk fibroin aqueous solution, and then use a freeze dryer to dry the solution to obtain a pure silk fibroin dry product.

[0062] The prepared dried silk fibroin was dissolved in hexafluoroisopropanol to obtain a 0.5% (mass volume ratio) silk fibroin organic solution.

[0063] Finally, the silk fibroin na...

Embodiment 2

[0078] Put 100g raw silk into boiled 0.5% sodium carbonate solution, react for half an hour to remove the sericin on the surface. The degumming rate was measured by biuret reaction and weighing method, and the results confirmed that the silk surface sericin had been completely removed. Subsequently, the resulting degummed raw silk was rinsed with deionized water and dried for the next step.

[0079] Put 25g of dried degummed raw silk into 1000ml of CaCl at 70°C 2 :CH 3 CH 2 OH:H 2 O=1:2:8 (mol) ternary solvent reacted for 8h to obtain silk fibroin solution. Then use a semi-permeable membrane to remove salt and other impurities to obtain a pure silk fibroin aqueous solution, and then use a freeze dryer to dry the solution to obtain a pure silk fibroin dry product.

[0080] The prepared dried silk fibroin was dissolved in hexafluoroisopropanol to obtain a 4% (mass volume ratio) silk fibroin organic solution.

[0081] Finally, the silk fibroin fiber was prepared by utilizin...

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Abstract

The invention discloses a preparation method of fibroins of different forms. The method comprises the following steps of: removing a sericin part from a bave substance, and drying to obtain a degummed bave substance dried product; dissolving the degummed bave substance dried product, removing impurities to obtain a fibroin solution, and drying to obtain a fibroin dried product; dissolving the fibroin dried product into an organic solvent, and preparing a fibroin organic solution; and preparing fibroins of different forms including fibroin nanoparticles and fibroin fibers with a supercritical fluid forced dispersion solution. The preparation method disclosed by the invention is safe and simple, and has low cost; and obtained fibroin nanoparticles or fiber products have unique physical and chemical properties, and can be applied in the fields of spinning, biological medicine and the like.

Description

technical field [0001] The invention relates to the technical field of material preparation, in particular to a method for preparing silk fibroin in different forms. Background technique [0002] Silk fibroin from silkworm is a biomacromolecular protein composed of 5507 amino acids. The repeat structure (-Gly-Ala-Gly-Ala-Gly-Ser-) composed of glycine, alanine and serine (-Gly-Ala-Gly-Ala-Gly-Ser-), the main components of this protein, leads to the formation of antiparallel β-sheet structure in silk fibroblasts. Due to its good biocompatibility and degradability, moderate moisture absorption and moisturizing properties, and UV absorption function, silk fibroin powder has been widely used as an additive and substrate in the food industry and special cosmetics. In addition, silk fibroin has also been widely used in the field of biomedical materials as a drug-controlled release carrier in various forms including membranes, three-dimensional scaffolds, gels, electrospinning and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K14/435
CPCY02P20/54
Inventor 李翼赵政胡军岩陈爱政
Owner THE HONG KONG POLYTECHNIC UNIV
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