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Detection method for nitrofuran antibiotics

A nitrofuran and detection method technology, applied in the field of chemical detection and analysis, can solve the problems of complex operation, high cost, long measurement time, etc., and achieve the effect of high sensitivity

Active Publication Date: 2019-03-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have the disadvantages of long measurement time, complicated operation and high cost.

Method used

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  • Detection method for nitrofuran antibiotics
  • Detection method for nitrofuran antibiotics
  • Detection method for nitrofuran antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The present embodiment has investigated altogether 7 big classes, altogether 18 kinds of antibiotic drugs are taken as research object, and these substances include: nitrofuran drugs (nitrofurantoin, nitrofurazone, furazolidone), sulfonamide drugs (sulfadiazine, sulfamethazine, sulfathiazole ), nitroimidazoles (metronidazole, ornidazole, dimetidazole), chloramphenicols (thiamphenicol, chloramphenicol), β-lactam antibiotics (ampicillin), large Cyclic lactones (roxithromycin) and imidazoles (fluconazole, clotrimazole, miconazole nitrate) and so on.

[0042] The selectivity test for antibiotic drug detection using the above-mentioned POSS fluorescent porous polymer dispersion:

[0043] (1) Take a certain quality of the above-mentioned different antibiotic drugs, add anhydrous grade tetrahydrofuran respectively to obtain a sample solution of 1mM / L for subsequent use;

[0044] (2) Put the POSS fluorescent porous polymer dispersion in the cuvette and put it into the sample p...

Embodiment 2

[0047] This embodiment provides a detection method for nitrofuran antibiotics. The sample to be tested is added to the above-mentioned POSS fluorescent porous polymer dispersion and mixed, and the sample to be tested is obtained by analyzing the change of the fluorescence signal intensity before and after adding the sample to be tested. The concentration of the nitrofuran antibiotics to be tested.

[0048] Its specific steps are:

[0049] (1) get the sample to be tested and make the sample to be tested for subsequent use, and prepare the standard samples of the nitrofuran antibiotics to be measured in different concentrations for subsequent use;

[0050] (2) Put the POSS fluorescent porous polymer dispersion into the sample pool of the fluorescence spectrophotometer to scan, record the fluorescence intensity, and add standard samples of different concentrations into the POSS fluorescent porous polymer dispersion each time. The fluorescence intensity that produces is detected ...

Embodiment 3

[0057] This embodiment tests the recycling performance of the POSS fluorescent porous polymer:

[0058] The prepared POSS fluorescent porous polymer dispersion was added to a fluorescent cuvette, and the degree of fluorescence quenching after adding the sample was recorded. The POSS fluorescent porous polymer was collected by centrifugation and washed several times with anhydrous THF, and then a new round of detection process was performed. like Figure 4 It can be seen that the recycling performance of the material is relatively stable after 9 cycles, and the fluorescence quenching rate of nitrofurazone still remains above 70% (RSD=5.8%).

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Abstract

The invention relates to a detection method for nitrofuran antibiotics. The detection method is characterized by measuring the concentrations of to-be-detected nitrofuran antibiotics in to-be-detectedsamples by adoption of a fluorescence quenching method by taking a POSS fluorescent porous polymer as a fluorescence material. According to the detection method, the fluorescence quenching method isadopted to rapidly detect the nitrofuran antibiotics in the to-be-detected samples, so that the detection method has the advantages of being high in sensitivity, good in selectivity, less in sample size and simple in method, and has practical application value for the detection of the nitrofuran antibiotics.

Description

technical field [0001] The invention relates to the technical field of chemical detection and analysis, in particular to a rapid detection method for nitrofuran antibiotics. Background technique [0002] Nitrofuran drugs are a class of synthetic antibacterial drugs, which include three main classes: nitrofurantoin, nitrofurantoin, and furazolidone. It acts on the microbial enzyme system, inhibits acetyl coenzyme A, and interferes with the metabolism of microbial sugars, thereby acting as a bacteriostasis. It is widely used to treat infectious diseases caused by Escherichia coli or Salmonella, and it can also promote the growth of animals, so it can be used as a feed additive. However, nitrofuran drugs are a class of potentially carcinogenic and mutagenic substances, and long-term use is prone to toxic reactions. For safety reasons, my country promulgated a ban on the use of nitrofuran antibiotics. There are many detection methods for nitrofuran antibiotics, mainly microbi...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N21/643G01N2021/6432G01N2021/6417
Inventor 李攻科周佩佩路振宇张润坤
Owner SUN YAT SEN UNIV
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