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Method for measuring protein nitrogen content in tobaccos

A determination method and technology for nitrogen content, which are applied in biological testing, preparation of test samples, material inspection products, etc., can solve the inaccurate results of protein nitrogen content determination, incomplete and incomplete digestion, and affect the digestion of nitrogen-containing components. and other problems, to achieve the effect of high accuracy, simple steps, and short processing cycle.

Active Publication Date: 2014-03-26
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above-mentioned high-pressure digestion has a good digestion effect on inorganic nitrogen, but the nitrogen-containing components in tobacco are complex, and this high-pressure digestion method cannot completely digest certain nitrogen-containing compounds in tobacco such as plant alkaloids, amino acids, and water-soluble proteins. and thoroughly, the obtained digestion effect is not uniform, which brings large errors; and tobacco contains reducing components, such as sugars, which will react with oxidants, thereby affecting the digestion of nitrogen-containing components, seriously affecting Its digestion effect makes the determination of protein nitrogen content in tobacco inaccurate

Method used

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  • Method for measuring protein nitrogen content in tobaccos
  • Method for measuring protein nitrogen content in tobaccos
  • Method for measuring protein nitrogen content in tobaccos

Examples

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Effect test

Embodiment 1

[0069] 11 g of sodium nitrate was dissolved in 100 mL of distilled water to obtain a sodium nitrate stock solution with a mass concentration of 10.0%. Accurately pipette 1.0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL of the standard stock solution, and use 0.5% acetic acid solution to dilute it to 100mL respectively to obtain a series of sodium nitrate standards solution to be tested. Take 2 mL of the standard solution to be tested respectively, pour it into the sample tube of the continuous flow analyzer and measure it in parallel three times respectively, and get the average value. In the continuous flow analyzer, the sample injection volume is 0.1mL / min, the injection time is 100s, and the flushing time is 120s. First at 90°C, add 2mL of potassium persulfate solution with a molar concentration of 0.3mol / L in the standard solution to be tested, the injection volume of potassium persulfate solution is 0.8mL / min, then the mixed solution obtained at 90 ℃, under the acidic conditio...

Embodiment 2～17

[0075] According to the standard GB / T19616-2004 of the People's Republic of China, 16 kinds of tobacco samples were selected. The tobacco samples were the flue-cured tobacco leaf samples produced at home and abroad in 2006, 2007 and 2008 provided by the tobacco leaf group of the raw material inspection room of the company's technology center. According to the standards of the People's Republic of China Tobacco industry standard YC / T31-1996 prepares tobacco dust samples and determines the moisture content in the tobacco dust. Dissolve 2g of smoke powder sample in 100mL of 0.5% acetic acid solution by volume fraction, heat to boil for 15 minutes, filter quickly with nitrogen-free qualitative filter paper, wash the precipitate with 0.5% acetic acid solution by volume fraction, combine the obtained filtrates, After cooling, set the volume to 200mL, accurately pipette 2mL of the filtrate and pour it into the sample tube of the continuous flow analyzer for three parallel measurements...

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Abstract

The invention provides a method for measuring the protein nitrogen content in tobaccos. The method comprises the following steps of: separating protein nitrogen in the tobaccos to obtain a sample to be measured, wherein the sample to be measured contains non-protein nitrogen; adding potassium peroxydisulfate to the sample to be measured under the heating condition to obtain a mixed solution; carrying out ultraviolet catalysis and high-temperature heating on the mixed solution in sequence to obtain a digestion product; detecting the digestion product to obtain the non-protein nitrogen content in the tobaccos; and obtaining the protein nitrogen content in the tobaccos according to the preset total nitrogen content in the tobaccos and the non-protein nitrogen content in the tobaccos. The method provided by the invention has the capability of digesting nitrogenous substances in the tobaccos more completely and thoroughly and has a favorable digestion effect; and according to the method provided by the invention, reducible components in the tobaccos are also digested at the same time that the nitrogenous substances in the tobaccos are digested, so that the tobaccos lose the reducing action, the interference of the reducing components to the digestion of the nitrogenous substances is reduced, the homogeneity of digestion results is improved, and the accuracy of measuring the protein content in the tobaccos is also improved.

Description

technical field [0001] The invention relates to the technical field of analyzing and measuring tobacco components, in particular to a method for measuring protein nitrogen content in tobacco. Background technique [0002] The protein in tobacco has a great influence on the quality of tobacco. Generally speaking, the protein content is inversely proportional to the quality of tobacco. The protein content of high-quality tobacco is below 10%. Protein is one of the precursors of many nitrogen-containing compounds in smoke, and the reduction of protein content in tobacco contributes to the reduction of N-heterocyclic compounds (quinoline, N-heterocyclic amines), aromatic amines in Hofmann smoke analytes and HCN, so a lower content of protein has a positive impact on the quality of tobacco; but if the protein content is too low, the smoke produced will appear flat, and the taste and aroma will also deteriorate. The protein content directly affects the quality of tobacco . Moreo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N1/44
Inventor 孔浩辉程志颖张心颖马青
Owner CHINA TOBACCO GUANGDONG IND
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