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Tissue engineering scaffold material of chemical bonding active material and preparation method thereof

A tissue engineering scaffold and technology of active substances, applied in the field of biological scaffold materials and their preparation, can solve the problems of limited application, lack of hydrophobicity and lack of signal for cell recognition, etc.

Inactive Publication Date: 2012-07-18
江西欧芮槿生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the hydrophobicity of the surface of polyester materials and the lack of cell recognition signals, the application of such materials in the field of tissue engineering is limited.

Method used

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  • Tissue engineering scaffold material of chemical bonding active material and preparation method thereof
  • Tissue engineering scaffold material of chemical bonding active material and preparation method thereof
  • Tissue engineering scaffold material of chemical bonding active material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Configuration of polymer spinning solution: PCL (molecular weight: 100,000) and collagen (molecular weight: 100,000) are mixed and dissolved in trifluoroethanol to prepare a polymer spinning solution with a total concentration of 12w / v%. , wherein the mass ratio of PCL to collagen is 60:40;

[0047] (2) Preparation of nanofiber membrane material: the polymer spinning liquid obtained in step (1) is loaded into the liquid storage device of a single-nozzle electrospinning equipment for electrospinning, and the electrospinning process parameters are: static voltage is 20kV; The feeding rate is 50ul / min, the receiving distance is 15cm; the ambient temperature is 20°C, and the polymer fiber membrane material is collected on the collector, and the average diameter of the fiber is 550nm.

[0048] (3) Soak the polymer fiber membrane material obtained in step (2) into a mixed solution of 80 / 20 ethanol and water containing 100mM carbodiimide, cross-link the GE component, and r...

Embodiment 2

[0052] (1) Configuration of polymer spinning solution: dissolve PLGA (molecular weight: 100,000) in a mixed solvent of 80 / 20 DMF and acetone to obtain a PLGA solution with a concentration of 12w / v%; dissolve hyaluronic acid and gelatin In a 90 / 10 mixed solvent of ethanol and water, a mixed solution with a concentration of 5w / v% and a mass ratio of hyaluronic acid and gelatin of 30 / 70 is prepared.

[0053] (2) Preparation of nanofiber membrane material: use a multi-nozzle spinning device, fill the mixed solutions of PLGA solution, hyaluronic acid and gelatin into different infusion channels for electrospinning, and the electrospinning process parameters are: The voltage is 25kV; the feeding rate is 20ul / min, the receiving distance is 8cm; the ambient temperature is 50°C, and the polymer fiber membrane material is obtained on the collector, and the diameter of the fiber is between 250-1000nm.

[0054] (3) Soak the fibrous membrane obtained in step (2) into a mixed solution of 80...

Embodiment 3

[0058] (1) Preparation of synthetic polymer nanofiber membrane: PCL is dissolved in the mixed solvent of DMF and methanol of 80 / 20, and is prepared into a PCL solution with a concentration of 12W / V% for electrospinning. The electrospinning process parameters are: The static voltage is 15kV; the feeding rate is 10ul / min, the receiving distance is 8cm; the ambient temperature is 20°C, and a 2-6mm thick PCL nanofiber membrane material is obtained on the collector.

[0059] (2) Alkali treatment: prepare 0.1 M sodium hydroxide aqueous solution, place the PCL nanofiber membrane material in the solution, react at 0° C. for 20 minutes, and rinse with a large amount of deionized water.

[0060] (3) Typing of gelatin macromolecules: the PCL nanofiber membrane material obtained in step (2) is put into a concentration of 200 mM dehydrating agent carbodiimide, and after reacting at room temperature for 16 hours, soak and rinse with a large amount of deionized water , and then soaked in a g...

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Abstract

The invention provides a tissue engineering scaffold material of a chemical bonding active material and a preparation method of the tissue engineering scaffold material. The tissue engineering scaffold material of the chemical bonding active material is prepared by a biodegradable macromolecular substance and an active material, wherein the macromolecular substance is counted by 100 parts by weight, and the active material is 0-15 part(s) by weight but is not zero; the 100 parts by weight of the macromolecular substance consists of 50-100 parts by weight of the synthesized macromolecular substance and 0-50 part(s) by weight of natural macromolecular substance; and a macromolecular nano-fiber film or a composite macromolecular nano-fiber film is formed by the macromolecular substance by means of electrostatic spinning, and the active material is evenly bonded on the surface of the fiber film. The tissue engineering scaffold material of the chemical bonding active material provided by the invention is good in biocompatibility, enough in mechanical strength, biodegradable, and suitable for stem cell proliferation and directed differentiation, and has the functions of cell migration adhesion promotion and stem cell capture to induce the regeneration of tissues such as bone, cartilage, nerve, skin, etc.

Description

technical field [0001] The invention relates to a biological material, in particular to a biological scaffold material capable of trapping stem cells, inducing stem cell proliferation and directional differentiation, and inducing tissue regeneration, as well as its preparation method and medical application. Background technique [0002] In recent years, the concept of tissue-inducing biomaterials has gradually formed in the field of biomaterials science. The key to human tissue regeneration and functional reconstruction is to design scaffolds with high bioresponsive activity that can effectively induce human tissue regeneration and repair behavior. For tissue engineering scaffolds, since most biological reactions occur on the surface or interface, the surface properties and structure of biomaterials are extremely important for the biocompatibility of materials. The surface properties of the implant material determine its adsorption to biomacromolecules in the body, especia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/18A61L27/20A61L27/22A61L27/24
Inventor 聂华荣邓志锋汪泱易应萍郭菲
Owner 江西欧芮槿生物科技有限公司
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