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A kind of compound enzyme for scouring cotton fabric and its preparation and application

A cotton fabric, compound enzyme technology, applied in the fields of biochemical equipment and methods, textiles and papermaking, enzymes, etc., can solve the problems of cotton cloth damage and strength reduction, and achieve low cellulase activity, mild reaction conditions, and environment-friendly conditions. Effect

Active Publication Date: 2018-02-23
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, we have made some progress in degrading cottonseed hulls with the compound enzyme crude enzyme solution produced by the fermentation of microorganisms that produce lignocellulose-degrading enzymes. However, due to the high activity of cellulase in the compound enzyme, it has caused certain damage to cotton cloth. , strong drop

Method used

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  • A kind of compound enzyme for scouring cotton fabric and its preparation and application
  • A kind of compound enzyme for scouring cotton fabric and its preparation and application
  • A kind of compound enzyme for scouring cotton fabric and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take a certain amount of microcrystalline cellulose and carboxymethyl cellulose (CMC), add 0.01g, 0.02g, 0.05g, 0.1g and 0.2g according to each unit of cellulase activity (U), pour Put it into the compound enzyme (ZL200710171935.6 or CN101974493A) obtained by fermentation of microorganisms such as Trichoderma reesei, and stir at room temperature for 30 minutes. The cellulase activity and xylanase activity of the supernatant and filtrate were measured after centrifugation or filtration under the condition of 5000g, and the experimental results are shown in figure 1 .

[0028] figure 1It shows the adsorption of different types of cellulose to the complex enzyme. From the two figures A and B, we can easily find that when the adsorption time is 30 minutes, with the increase of the amount of the two celluloses, the two enzymes The adsorption rate of microcrystalline cellulose also increases, the maximum adsorption rate of microcrystalline cellulose to cellulase is about 29...

Embodiment 2

[0030] The commercially available compound enzyme (Ningxia Hersbit Biotechnology Co., Ltd.) was treated with 0.05 g / U of microcrystalline cellulose, and stirred at room temperature for 15-90 min. The cellulase activity and xylanase activity of the supernatant and filtrate were measured after centrifugation or filtration under the condition of 5000g, and the experimental results are shown in figure 2 .

[0031] The results show that with the increase of adsorption time, the adsorption of microcrystalline cellulose to cellulase does not change regularly, but the amount of adsorption to xylanase has been increasing, indicating that the binding of microcrystalline cellulose to cellulase is changing. , but it is more firmly bound to xylanase. It can be seen from the figure that when the adsorption time is 15 min and 60 min, the adsorption amount of microcrystalline cellulose to cellulase reaches a maximum of about 28%, and the adsorption amount to xylanase is only 0.6%. Therefor...

Embodiment 3

[0033] Using properly treated corncobs and cottonseed hulls as inducers, the purchased Trichodermareesei Rut C30 was submerged in liquid culture, and the crude enzyme solution after four days of fermentation was collected. The crude enzyme solution was treated with 0.05 g / U of microcrystalline cellulose, stirred at room temperature for 15 minutes and absorbed, then the enzyme activity of the enzyme solution was measured. The results are shown in Table 1.

[0034] Table 1 Changes in the activity of the compound enzyme before and after adsorption

[0035]

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Abstract

The invention relates to a cotton fabric scouring compound enzyme and its preparation and application. The cotton fabric scouring compound enzyme comprises: xylanase, pectinase, cellulase, lipase and protease; its preparation method, It includes: adding cellulose to the compound enzyme crude enzyme liquid synthesized by fermentation of enzyme-producing microorganisms or passing through a cellulose-containing chromatographic column, separating and removing the cellulose after adsorption treatment or collecting the components flowing out of the chromatographic column, and the product is obtained; its application It includes: dilute the cotton fabric scouring compound enzyme, mix it with cotton fabric, and shake it in a water bath under the conditions of pH 2.0-10.0, 20-80°C, and 100-200rpm; wash and bleach after the reaction is completed. The composite enzyme prepared by the present invention has lower cellulase activity, higher xylanase activity and pectinase, and is more suitable for enzyme refining of cotton fabrics to remove impurities such as cottonseed hulls; the method operation of the present invention Simple, highly controllable and environmentally friendly.

Description

technical field [0001] The invention belongs to the field of fabric scouring compound enzyme and its preparation and application, in particular to a cotton fabric scouring compound enzyme and its preparation and application. Background technique [0002] At present, in order to meet the low-carbon economic requirements of my country's traditional industrial transformation and energy conservation and emission reduction, the textile industry is developing a new pretreatment process for cotton fabric biological enzymatic refining. Due to the low quality of domestic cotton, the removal of cottonseed hulls has become a major technical difficulty in enzyme scouring, which seriously hinders the industrial application of enzyme scouring technology. The chemical composition of cotton fiber symbiosis and cotton seed hulls is complex, and enzymes have high substrate catalytic specificity, and different types of enzymes can only degrade different impurity components. Enzymes are used t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50C12N9/42C12N9/26C12N9/20D06M16/00C12R1/885C12R1/66C12R1/80C12R1/77C12R1/785C12R1/645C12R1/07C12R1/125C12R1/38C12R1/465C12R1/01
Inventor 洪枫黄伟
Owner DONGHUA UNIV