Compound extracted from Italian oriental cocklebur and application thereof as weedicide
A technology of Italian cocklebur, herbicide, applied in the direction of herbicide and algicide, application, biocide, etc.
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Embodiment 1
[0036] Embodiment one: the extraction of cocklebur saponin
[0037] The present invention specifically provides a method for extracting cocklebur saponin from the harmful plant cocklebur, the specific extraction steps are as follows:
[0038] (1) Grinding the Italian cocklebur fruit with a pulverizer after drying: first collect the mature Italian cocklebur plant, separate the fruit from other parts, and smash it with a pulverizer after drying for subsequent use;
[0039] (2) Soak several days and concentrate with five times volume of 95% ethanol: soak seven days at room temperature with 95% five times volume of crushed Italian cocklebur fruit; filter the soaking solution to remove residue, and then Evaporate with a rotary evaporator until there is no alcohol smell, and 95% ethanol is a commonly used ethanol reagent;
[0040] (3) The concentrated substance is degreased with petroleum ether: take the above-mentioned concentrated extract and stir it with warm water to obtain a s...
Embodiment 2
[0044] Example 2: Comparison of the effects of cocklebur saponin and commercial herbicides
[0045] Specific experimental method: the purified cocklebur saponin was formulated with chloroform to a concentration of 1 mg / ml, and then diluted to 8 μg / ml, 40 μg / ml, and 200 μg / ml in sequence. Filter paper was placed in a petri dish with a diameter of 9 cm, and then Add 3ml of cocklebur saponin solutions with different concentrations, 8 μg / ml, 40 μg / ml, 200 μg / ml, 1000 μg / ml in turn. After the chloroform is completely volatilized, add 3ml of distilled water to each petri dish, and add 10 amaranth, lettuce, wheat, ryegrass seeds to each petri dish; seal it with a sealing film and place it at 25°C for cultivation After cultivating in the box for 4 days, measure amaranth, lettuce, wheat seedling height and root length, measure ryegrass seedling height and root length after 5 days; adopt commercial herbicide promethazine to test with the same method.
[0046] Statistical method of data...
Embodiment 3
[0049] Example 3: Application of cocklebur saponin as a herbicide.
[0050] Specific experimental method: After suspending the pure cocklebur saponin provided by the present invention with water, add it to a flower pot with a diameter of 20 cm at a ratio of 5 mg / kg of soil to conduct a pot experiment. The plants used in the experiment were amaranth, lettuce, wheat, and ryegrass. Conventional irrigation, no fertilization. Observations were made several weeks later.
[0051] Results: The dicotyledonous lettuce basically stopped growing one week after germination, the leaves appeared withered and yellow, the root system died, and then the plants gradually died. See attached Figure 5 For cultivating lettuce seedlings after four weeks. The dicotyledonous radish showed a similar phenomenon, the seedlings grew poorly after emergence, the leaves withered and turned yellow, the root system died first, and the plants died later. See attached Image 6 For the radish seedlings afte...
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