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Extraction method for high acyl gellan gum

A technology of high acyl gellan gum and extraction method, which is applied in the field of extraction of non-clear and transparent high acyl gellan gum, which can solve the problem of damage to the health of operators, instability of acyl groups to acids, and influence on product coagulation. Gel performance and other issues, to achieve the effect of improved gel and suspension performance, extended shelf life, and suitable for large-scale production

Inactive Publication Date: 2012-08-08
杭州健恒生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem with this process is that the process of high-temperature sterilization treatment of the fermentation broth greatly damages the acyl groups on the main chain of gellan gum, especially the glycerol groups, and the removal of acyl groups is serious, which affects the gel performance of the product.
This process uses a variety of non-polar solvents including acetone, ether or n-hexane to wash the gellan gum fiber, which not only damages the health of the operators, but also easily causes production accidents; in addition, the process adjusts the pH to a strong Acidity makes high acyl gellan gum flocculate and precipitate to achieve the purpose of solid-liquid separation, and the acyl group in high acyl gellan gum is also very unstable to acid and will be removed from the molecule when the pH value is lower than 2.0. Seriously affect the gel performance of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The high acyl gellan gum prepared in this example is non-clear type high acyl gellan gum, and the specific process steps are as follows:

[0029] (1) Pretreatment of fermentation broth: select gellan gum fermentation broth with a gel yield of 1.8% and a glue viscosity of 10000cp as the extraction raw material, slowly add potassium chloride accounting for 5% of the volume of the fermentation broth to the fermentation broth, add Pay attention to slowly adding in batches, and keep stirring rapidly, and keep stirring for 30 minutes, and slowly add acetic acid solution to adjust the pH value of the fermentation broth to 5.0;

[0030] (2) Extraction, washing, and solid-liquid separation of fermentation broth: add ethanol with a mass percentage concentration of 95% to the fermentation broth obtained in step (1), and the amount added is 1.5 times the initial volume of the fermentation broth. At this time, there is flocculent precipitation Formation, rapid agitation, extraction ...

Embodiment 2

[0034] The high acyl gellan gum prepared in this example is non-clear type high acyl gellan gum, and the specific process steps are as follows:

[0035] (1) Pretreatment of fermentation broth: select gellan gum fermentation broth with a gel yield of 1.6% and a glue viscosity of 8500cp as the extraction raw material, and slowly add sodium chloride with a volume of 8% of the fermentation broth to the fermentation broth. Pay attention to slowly adding in batches, and keep stirring rapidly, and keep stirring for 30 minutes, slowly add citric acid solution, and adjust the pH of the fermentation broth to 5.5;

[0036] The above-mentioned fermented liquid is passed through a ceramic membrane, concentrated under stirring conditions, and the volume of the fermented liquid obtained after concentration is 50% of its initial volume;

[0037](2) Extraction, washing, and solid-liquid separation of fermented broth: add isopropanol with a mass percent concentration of 96% to the fermented bro...

Embodiment 3

[0041] The high acyl gellan gum prepared in this example is transparent high acyl gellan gum, and the specific process steps are as follows:

[0042] (1) Pretreatment of fermentation broth: select gellan gum fermentation broth with a gel yield of 1.7% and a glue viscosity of 9000cp as the extraction raw material, slowly add potassium sulfate with a volume of 3% of the fermentation broth to the fermentation broth, and pay attention when adding Slowly add in batches, and keep stirring rapidly, keep stirring for 30 minutes, slowly add lactic acid solution, adjust the pH of the fermentation broth to 4.5;

[0043] The above-mentioned fermented liquid is passed through a ceramic membrane, concentrated under stirring conditions, and the volume of the concentrated fermented liquid obtained is 70% of its initial volume;

[0044] (2) Extraction, washing, and solid-liquid separation of fermented liquid: add lysozyme to the fermented liquid obtained in step (1), the concentration of lysoz...

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Abstract

The invention relates to an extraction method for high acyl gellan gum, which comprises the following process steps of adding alkali metal salt occupying 3%-8% of the volume of fermentation liquid into the fermentation liquid, adjusting the pH value, adding low alcohols with different concentration into the fermentation liquid, repeatedly performing extraction, washing and solid-liquid separation, obtaining high acyl gellan gum fibers, finally scattering the high acyl gellan gum fibers, drying and crushing the fibers at 90 DEG C-120 DEG C, and obtaining the high acyl gellan gum. The extraction process of the extraction method for the high acyl gellan gum is performed in a weak acid environment without high temperature and strong acid and alkali treatments, and has a good protection function on the stability of acyl groups. The gel and floating performance of the high acyl gellan gum is greatly improved, a final product also has weak acidity, and a good prolonging function on the guarantee period of the product is achieved. The extraction method for the high acyl gellan gum adopts a method of washing the low alcohols in a mode of small amount and multiple times, impurities and pigment in the high acyl gellan gum are removed thoroughly, and the appearance of the obtained product is greatly improved.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to an extraction method capable of producing non-clear type and transparent type high acyl gellan gum. Background technique [0002] Gellan gum is a kind of hydrophilic colloid developed by the American Keke (KELCO) company. Sphingomonas paucimobilis ) is a fermenting bacteria, after several stages of fermentation and cultivation, the fermented liquid is produced through a series of post-extraction processes. Gellan gum is one of the polysaccharides of the gellan family (Gellan Family). Its molecular basic structure is a main chain, which is composed of repeated tetrasaccharide units. The monosaccharides involved in the formation are glucose, rhamnose and glucuronic acid. , the molar ratio is 2:1:1. [0003] In the original form of gellan gum, that is, high acyl gellan gum, there are two substituents, namely, acetic acid acyl substituent and glycerol acyl substituent, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 许怀远陈波
Owner 杭州健恒生物技术有限公司
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