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Three-dimensional non-support bone repairing patch and preparation method thereof

A non-supporting, bone repair technology, applied in medical science, prosthesis, etc., can solve the problems of difficult cell planting and penetration, and achieve the effect of excellent biocompatibility, good biocompatibility, and large adsorption capacity

Inactive Publication Date: 2012-08-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the early stage, cells can easily migrate to the region of the electrospun fiber repair material at a depth of about 100 μm, but in the later stage, for deeper regions, it is difficult for cells to plant and infiltrate

Method used

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  • Three-dimensional non-support bone repairing patch and preparation method thereof
  • Three-dimensional non-support bone repairing patch and preparation method thereof
  • Three-dimensional non-support bone repairing patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of fiber scaffold

[0035] Dissolve PCL and PGA in trifluoroacetic acid at a mass ratio of 9:1 to prepare a 20% solution. Stir with a magnetic stirrer to obtain a transparent and uniform electrospinning solution;

[0036] The prepared electrospinning stock solution was electrospun, and the spinning parameters were controlled at a voltage of 15kv, a nozzle aperture of 0.4mm, a solution flow rate of 1ml / h, an ambient temperature of 20°C, and a distance of 15cm between the flat receiving device and the nozzle.

[0037] (2) Preparation of unsupported bone patch

[0038] After the electrospinning, the fiber support was removed from the flat receiving device, immersed in ethanol for 48 hours, and then vacuum-dried. Cut into a three-dimensional shape of length 5cm×width 5cm×thickness 0.5mm, and sterilize.

Embodiment 2

[0040] (1) Preparation of fiber scaffold

[0041] Dissolve PCL and PGA in trifluoroacetic acid at a mass ratio of 5:5 to prepare a 10% solution. Stir with a magnetic stirrer to obtain a transparent and uniform electrospinning solution;

[0042] The prepared electrospinning stock solution was electrospun, and the spinning parameters were controlled at a voltage of 20kv, a nozzle aperture of 0.7mm, a solution flow rate of 2ml / h, an ambient temperature of 25°C, and a distance between the winding drum (diameter of 50cm) and the nozzle of 5cm. The winding speed is controlled at 200rpm

[0043] (2) Preparation of unsupported bone patch

[0044] After electrospinning, the fiber support was removed from the winding drum, immersed in ethanol for 48 hours, and then vacuum-dried. Cut into a three-dimensional shape with a certain radian (length 5cm×width 5cm×thickness 0.5mm), and sterilize.

Embodiment 3

[0046] (1) Preparation of fiber scaffold

[0047] Dissolve PCL and PGA in hexafluoroisopropanol at a mass ratio of 5:5 to prepare a 5% solution. Stir with a magnetic stirrer to obtain a transparent and uniform electrospinning solution;

[0048]The prepared electrospinning stock solution was electrospun, and the spinning parameters were controlled at a voltage of 5kv, a nozzle aperture of 1mm, a solution flow rate of 0.5ml / h, an ambient temperature of 25°C, and a distance between the winding drum (diameter of 5cm) and the nozzle of 5cm. The winding speed is controlled at 4000rpm

[0049] (2) Preparation of unsupported bone patch

[0050] After electrospinning, the fiber support was removed from the winding drum, immersed in the organic solvent ethanol for 48 hours, and then vacuum-dried. It is cut into the three-dimensional shape of the repaired brain patch and sterilized for bone marrow mesenchymal stem cell culture.

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Abstract

The invention relates to a three-dimensional non-support bone repairing patch and a preparation method thereof. The patch is composed of nanometer fibre with an extracellular matrix, wherein the nanometer fibre is composed of polycaprolactone and polyglycolic acid which are blended in proportion by weight according to (1:9) to (9:1). The preparation method comprises the following steps of: dissolving two polymers in an organic solvent, and stirring to prepare a transparent and uniform electrostatic spinning solution; spinning the spinning solution by utilizing an electrostatic spinning process, and receiving to obtain a fibre support; and soaking, drying, tailoring and disinfecting the fibre support, and culturing by utilizing marrow mesenchmal stem cells to manufacture a three-dimensional bone patch repairing material. The three-dimensional non-support repairing patch provided by the invention can regulate pore diameter and void percentage at discretion according to need, can be used as nerve conduits and tissue engineering cytoskeletons of bones, blood vessels, hearts, nerves and the like, and is suitable for growth of multiple cells; and the preparation method has the advantages of simple process, low cost, and good application prospects.

Description

technical field [0001] The invention belongs to the field of medical materials and its preparation, in particular to a three-dimensional non-supporting bone repair patch and its preparation method. Background technique [0002] Polyglycolic acid (PGA) and polycaprolactone (PCL), represented by polyglycolic acid (PGA) and polycaprolactone (PCL), have good processability and plasticity, and the degradation performance and mechanical properties of the material can be adjusted in a wide range. They have been successfully used in bone regeneration. Scaffold materials, tissue engineering scaffolds such as heart, blood vessels and nerves [are used to support many regenerative tissues. Tissue-engineered scaffolds woven from PGA have placed chondrocytes and stem cells on top of the material to create a repairing heart patch that has been tested both in vivo and in vitro. However, due to the rapid degradation of PGA, it is difficult to maintain the mechanical stability, so its applic...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/38A61L27/50
Inventor 冯淑芹沈新元冯虎
Owner DONGHUA UNIV
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