Preparation method for phytosterol acetate formed by catalytic synthesis through enzymic method
A technology of phytosterol acetate and phytosterol, which is applied in the field of preparation of phytosterol acetate catalyzed by an enzymatic method, can solve problems such as safety hazards of acetic anhydride, safety of chemical synthesis methods, and environmental pollution, and achieve production The effect of short process flow, convenient industrial production and high esterification rate
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Embodiment 1
[0023] Add phytosterol and pyruvic acid with a molar ratio of 1:5 into a 150mL triangular flask, add 10mL of dehydrated n-hexane, put it in a water bath constant temperature oscillator, 55°C, fully shake and mix for 30min, and wait for the reaction Add 5%wt lipase (based on the mass of pyruvic acid) as a catalyst, 40mg / mL (the amount of molecular sieve added in the reaction solvent per unit volume is 40mg) molecular sieve as a dehydrating agent, and react under stirring conditions at 55°C Ends in 18 hours. After the reaction solution is cooled, filter the reaction solution with suction to separate the enzyme, molecular sieve and a small amount of unreacted sterols. If the filtrate still has a small amount of turbidity, it can be filtered again. According to the fact that phytosterol esters have a strong absorption peak at 640nm of visible light , Quantitative determination of the amount of phytosterol esters, the esterification rate was 93.01%.
Embodiment 2
[0025] Add phytosterol and pyruvic acid with a molar ratio of 1:5 into a 150mL triangular flask, add 10mL of dehydrated n-hexane, put it in a water bath constant temperature oscillator, 55°C, fully shake and mix for 30min, and wait for the reaction The substance was completely dissolved, and 6% lipase (based on the amount of pyruvate) was added as a catalyst, 40 mg / mL molecular sieve was used as a dehydrating agent, and the reaction was completed after 16 hours at 55°C under stirring conditions. After the reaction solution is cooled, filter the reaction solution with suction to separate the enzyme, molecular sieve and a small amount of unreacted sterols. If the filtrate still has a small amount of turbidity, it can be filtered again. According to the fact that phytosterol esters have a strong absorption peak at 640nm of visible light , Quantitative determination of the amount of phytosterol esters, the esterification rate was 91.25%.
Embodiment 3
[0027] Add phytosterol and pyruvic acid with a molar ratio of 1:4 into a 150mL triangular flask, add 10mL of dehydrated n-hexane, put it in a water bath constant temperature oscillator, 55°C, fully shake and mix for 30min, and wait for the reaction The substance was completely dissolved, and 5% lipase (based on the amount of pyruvate) was added as a catalyst, 40 mg / mL molecular sieve was used as a dehydrating agent, and the reaction was completed after 20 hours at 55°C under stirring conditions. After the reaction solution is cooled, filter the reaction solution with suction to separate the enzyme, molecular sieve and a small amount of unreacted sterols. If the filtrate still has a small amount of turbidity, it can be filtered again. According to the fact that phytosterol esters have a strong absorption peak at 640nm of visible light , Quantitative determination of the amount of phytosterol esters, the esterification rate was 87.90%.
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