Magnetic light-enzyme composite catalyst and preparation method thereof

A composite catalyst and magnetic technology, applied in the field of new magnetic photoenzyme composite catalysts, can solve the problems of immobilized enzymes and TiO2 being difficult to recover, etc.

Inactive Publication Date: 2012-10-17
INST OF PROCESS ENG CHINESE ACAD OF SCI
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jia et al. reported the use of porous glass beads immobilized laccase and TiO 2 The photocatalytic synergistic effect of phenolic catalysis can improve the catalytic effect of the sub-catalysis. The analysis shows that the enzyme catalysis and photocatalysis are carried out separately. The reason for the synergy may be that the free radical metalloenzyme catalytic pathway generated in the photocatalytic process accelerates the e

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Magnetic light-enzyme composite catalyst and preparation method thereof
  • Magnetic light-enzyme composite catalyst and preparation method thereof
  • Magnetic light-enzyme composite catalyst and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0077] Example 1

[0078] (1’) The prepared magnetic nano TiO 2 Nano particles are dispersed in deionized water to form a suspension, magnetic nano TiO 2 Fe 3 O 4 The content is 4% (w / w), magnetic nano TiO 2 The concentration is 2% (w / v), and then put it in the refrigerator for later use.

[0079] (1) Add 2g of silanization reagent, 120g of monoalcohol and magnetic nano TiO to the reaction system under the condition of stirring at room temperature (25℃) and 200 rpm. 2 4g of nanoparticles, 30g of water, adjust the pH to 5, and fully stir for 30 minutes to obtain the silanization reaction solution;

[0080] (2) The silanization reaction solution was reacted under nitrogen protection, water bath heating at 50°C, reflux device, and 120W ultrasonic conditions for 20 minutes. After magnetic separation, the silanized magnetic nano-TiO 2 Nanoparticle

[0081] (3) Silanized magnetic nano-TiO 2 Add nanoparticles to 2% (w / v) sodium iminodiacetate solution, the particle concentration is 4% (w / v), ...

Example Embodiment

[0086] Example 2

[0087] (1’) The prepared magnetic nano TiO 2 Nano particles are dispersed in deionized water to form a suspension, magnetic nano TiO 2 Fe 3 O 4 The content is 1% (w / w), and its concentration is 10% (w / v), and then put it in the refrigerator for later use;

[0088] (1) Add 2g of silanization reagent, 80g of monoalcohol, and magnetic nano TiO to the reaction system under the conditions of room temperature (25℃) and 200 rpm stirring. 2 1 g of nanoparticles, 20 g of water, adjust the pH to 3, and fully stir for 10 minutes to obtain the silanization reaction solution;

[0089] (2) The silylation reaction solution was reacted for 60 minutes under nitrogen protection, heated in a water bath at 30°C, equipped with a reflux device, and 60W ultrasonic conditions to obtain silanized magnetic nano-TiO 2 After the nanoparticles are magnetically separated, silanized magnetic nano-TiO 2 Nanoparticle

[0090] (3) Silanized magnetic nano-TiO 2 Add the nanoparticles to 0.5% (w / v) sodi...

Example Embodiment

[0095] Example 3

[0096] (1’) The prepared magnetic nano TiO 2 Nano particles are dispersed in deionized water to form a suspension, magnetic nano TiO 2 Fe 3 O 4 The content is 2% (w / w), and its concentration is 5% (w / v), and then put it in the refrigerator for later use;

[0097] (1) Add 2g of silanization reagent, 100g of monoalcohol, and magnetic nano TiO to the reaction system under the conditions of room temperature (25℃) and stirring at 300 rpm. 2 2.5 g of nanoparticles, 25 g of water, adjust the pH to 4, and fully stir for 20 minutes to obtain a silanization reaction solution;

[0098] (2) The silylation reaction solution was reacted for 40 minutes under nitrogen protection, heated in a water bath at 40°C, equipped with a reflux device, and 90W ultrasonic conditions to obtain silanized magnetic nano-TiO 2 After the nanoparticles are magnetically separated, silanized magnetic nano-TiO 2 Nanoparticle

[0099] (3) Silanized magnetic nano-TiO 2 Nanoparticles are added to 1% (w / v) s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to a magnetic light-enzyme composite catalyst and a preparation method thereof. The magnetic light-enzyme composite catalyst is laccase immobilized on magnetic nano TiO2 processed by surface functionalization, particularly laccase immobilized on magnetic nano TiO2 particles modified by copper ion chelating. According to the invention, the magnetic light-enzyme composite catalyst provided by the invention realizes the transmission of electrons between laccase and TiO2 in the catalytic oxidation process, solves the problems of slow speed of receiving electrons by laccase from the substrate and slow combination between photoinduced electrons and O2; by using the synergism of enzyme catalysis and photocatalysis to raise catalytic oxidation efficiency, the oxidation of substrates with high oxidation-reduction potential and macromolecule substrates can be realized; the catalyst has superparamagnetism, can be conveniently and effectively controlled and separated under the effect of external magnetic field, the preparation process is simple, and the catalyst is suitable for large scale production.

Description

technical field [0001] The invention relates to a novel magnetic photoenzyme composite catalyst, in particular to a magnetic photoenzyme composite catalyst which couples titanium dioxide photocatalysis with an electron transfer chain catalyzed by laccase enzymes and a preparation method thereof. Background technique [0002] Laccase (benzenediol: oxidoreductase, EC 1.10.3.2) is a copper-containing polyphenol oxidase, which belongs to the same blue multi-copper oxidase as plant ascorbate oxidase and mammalian ceruloplasmin enzyme family. Laccase can convert O through 4 electrons 2 Catalytic reduction to water with concomitant oxidation of some phenolic substrates. The catalytic reaction of laccase oxidation of phenols, aromatic amines and other substances has the advantages of mild reaction conditions, high selectivity and high catalytic efficiency. Laccase is one of the most promising redox enzymes, mainly used in organic synthesis, degradation of environmental pollutants...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B01J31/38B01J31/40A62D3/02
Inventor 刘春朝王锋郭晨
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap