Magnetic light-enzyme composite catalyst and preparation method thereof

Abstract

The invention relates to a magnetic light-enzyme composite catalyst and a preparation method thereof. The magnetic light-enzyme composite catalyst is laccase immobilized on magnetic nano TiO2 processed by surface functionalization, particularly laccase immobilized on magnetic nano TiO2 particles modified by copper ion chelating. According to the invention, the magnetic light-enzyme composite catalyst provided by the invention realizes the transmission of electrons between laccase and TiO2 in the catalytic oxidation process, solves the problems of slow speed of receiving electrons by laccase from the substrate and slow combination between photoinduced electrons and O2; by using the synergism of enzyme catalysis and photocatalysis to raise catalytic oxidation efficiency, the oxidation of substrates with high oxidation-reduction potential and macromolecule substrates can be realized; the catalyst has superparamagnetism, can be conveniently and effectively controlled and separated under the effect of external magnetic field, the preparation process is simple, and the catalyst is suitable for large scale production.

Description

technical field [0001] The invention relates to a novel magnetic photoenzyme composite catalyst, in particular to a magnetic photoenzyme composite catalyst which couples titanium dioxide photocatalysis with an electron transfer chain catalyzed by laccase enzymes and a preparation method thereof. Background technique [0002] Laccase (benzenediol: oxidoreductase, EC 1.10.3.2) is a copper-containing polyphenol oxidase, which belongs to the same blue multi-copper oxidase as plant ascorbate oxidase and mammalian ceruloplasmin enzyme family. Laccase can convert O through 4 electrons 2 Catalytic reduction to water with concomitant oxidation of some phenolic substrates. The catalytic reaction of laccase oxidation of phenols, aromatic amines and other substances has the advantages of mild reaction conditions, high selectivity and high catalytic efficiency. Laccase is one of the most promising redox enzymes, mainly used in organic synthesis, degradation of environmental pollutants...

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Problems solved by technology

Jia et al. reported the use of porous glass beads immobilized laccase and TiO 2 The photocatalytic synergistic effect of phenolic catalysis can improve the catalytic effect of the sub-catalysis. The analysis shows that the enzyme catalysis and photocatalysis are carried out separately. The reason for the synergy may be that the free radical metalloenzyme catalytic pathway generated in the photocatalytic process accelerates the enzyme catalysis. , although this method achieves laccase catalysis and TiO 2 Photocatalytic synergy, but failed to resolve the rate-limiting step in their respective catalysis, and immobilized enzyme and TiO 2 Not easy to recycle (Degradation of high concentration 2,4-dichlorophenol by simultaneous photocatalytic–enzymatic process using TiO2 / UV and laccase, Jingbo Jia, etc., Journal of Hazardous Materials, 2012,205-206:150-155)

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