Trichoderma harzianum strain, and its application in control of potato late blight

A potato late blight, potato late blight technology, applied in the application, chemicals for biological control, fungicides, etc., can solve the problems of easily polluted environment, fungicide resistance and even drug resistance, and high chemical control costs, To achieve the effect of broad application prospects, increase farmers' income, and promote rapid development

Active Publication Date: 2012-10-17
CHINA AGRI UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high cost of chemical control and easy pollution of the environment, and the pathogens are easy to develop resistance or even resistance to fungicides

Method used

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  • Trichoderma harzianum strain, and its application in control of potato late blight
  • Trichoderma harzianum strain, and its application in control of potato late blight
  • Trichoderma harzianum strain, and its application in control of potato late blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the acquisition and identification of Trichoderma harzianum HNA-14

[0056] 1. Obtainment of strain HNA-14

[0057] 1. Sample collection

[0058] The samples were collected from the surface rhizosphere soil of potato crop fields in Anyang, Henan.

[0059] Remove the 3-5cm topsoil layer in the field with soil-borrowing equipment, dig out the soil together with the roots of the plants, pack them in polyethylene plastic bags, and bring them back to the laboratory for separation.

[0060] 2. Obtainment of strain HNA-14

[0061] Air-dry the root system that adheres to the soil, pat the root system to make the soil adhered to it fall off, dilute it with sterile water gradient, absorb 0.1mL of the diluted solution and drop it on the TSM medium plate, and use a sterilized L-shaped coating The cloth bar is evenly coated, and placed in a constant temperature incubator at 25-28°C for 3-4 days. Pick a single colony that resembles Trichoderma and transfer it to a P...

Embodiment 2

[0068] Embodiment 2, the inhibitory action of Trichoderma harzianum HNA-14 to pathogenic bacteria

[0069] Experimental group: Trichoderma harzianum HNA-14 bacteria cake and pathogenic bacteria cake were inoculated on the same PDA plate for confrontation culture (the vertical distance between the centers of the two bacteria cakes was 3cm, and the diameters of the two bacteria cakes were both 5mm); The PDA plate of the pathogenic bacteria cake was used as the control group; after 3 days of dark cultivation at 25°C, the colony diameter of the pathogenic bacteria (the average of the longest diameter and the shortest diameter) was measured and the inhibition rate was calculated. Inhibition rate = (colony diameter of pathogenic bacteria in the control group - colony diameter of pathogenic bacteria in the experimental group) / colony diameter of pathogenic bacteria in the control group * 100%. Three repeated treatments were set for each pathogenic bacteria, and three repeated treatm...

Embodiment 3

[0074] Embodiment 3, the greenhouse biocontrol effect experiment of Trichoderma harzianum HNA-14

[0075] After the potato cubes are germinated, select germinated seed potatoes with consistent growth and sow them in the cultivation pot, 10 seeds per pot; cultivate them in the greenhouse (25-30°C) until the two compound leaves of the seedlings are flattened, and perform the following operations in groups (3 for each group) Bowl):

[0076] Experimental group: Each potato seedling was inoculated with 15 mL of Trichoderma harzianum HNA-14 spore suspension (spore concentration of 5×10 6 individual / mL) and 15 mL of spore suspension of Phytophthora infestans (spore concentration of 5×10 6 per mL); the inoculation method is to inject the spore suspension into the rhizosphere soil with a syringe;

[0077] Control group: each potato seedling was inoculated with 15 mL of spore suspension of P. infestans (spore concentration of 5×10 6 per mL); the inoculation method is to inject the sp...

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Abstract

The invention discloses a Trichoderma harzianum strain, and its application in the control of potato late blight. The Trichoderma harzianum strain HNA-14 provided in the invention has a preservation number of CGMCC No.5990. The Trichoderma harzianum strain HNA-14 can be used for inhibiting pathogens, promoting the growth of potato plants, and controlling the potato late blight generation of the potato plants. The Trichoderma harzianum strain has a wide application prospect in the sustainable agriculture development.

Description

technical field [0001] The invention relates to a Trichoderma harzianum strain and its application in preventing and controlling potato late blight. Background technique [0002] Potatoes are the fourth largest food crop in the world and an important food and vegetable crop in China. Because of its high economic value and rich nutrition, it is widely cultivated all over the world. my country has become the world's largest potato producer. Potato production in my country is mainly distributed in Southwest, North China, Northwest, Northeast, Central China, East China and other regions. According to statistics, the potato cultivation area in my country in 2010 has exceeded 6 million ha 2 , the output accounts for 1 / 4 of the world. [0003] Potato late blight is a destructive disease in potato production, which generally occurs all over the world, generally causing a 20% to 80% reduction in potato production, and severe cases can result in complete failure. After the 1980s,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/885
Inventor 王琦姚彦坡李燕
Owner CHINA AGRI UNIV
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