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Method for performing surface treatment on material for boosting cell growth and resisting biological pollution

An anti-biological pollution and surface treatment technology, applied in the direction of coating, can solve the problems of low graft density and difficult to increase graft density, and achieve the effects of improving graft density, control of reaction conditions, and good anti-biological pollution performance.

Inactive Publication Date: 2014-05-07
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many molecules have been found to have anti-biofouling properties. Hydrophilic molecules usually have certain anti-biofouling properties. However, after these molecules are grafted on the surface of the material, the grafting density is often low and difficult to increase. Therefore, its wide application is limited. The use of surface in situ polymerization can increase the grafting density while maintaining the biological activity of immobilized molecules, thereby improving the anti-biofouling properties of the material surface.

Method used

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  • Method for performing surface treatment on material for boosting cell growth and resisting biological pollution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: according to the following steps, the surface of the material is treated

[0024] (1) Material surface activation: Cut the Ti6Al4V material into discs with a diameter of 1.5 cm and a thickness of 1 mm. After the surface is polished to a mirror surface, it is ultrasonically cleaned with acetone, ethanol, and distilled water for 10 minutes, and immersed in a mixed solution of concentrated sulfuric acid and hydrogen peroxide. Medium (sulfuric acid: hydrogen peroxide = 70:30 (v / v)) was treated for 30 minutes, then taken out, then washed with a large amount of deionized water, dried for later use;

[0025] (2) Material surface bromination: firstly prepare a toluene solution of 3-bromopropyltrimethoxysilane with a concentration of 10mM, immerse the surface-activated Ti6Al4V disk into 20ml of the prepared silane solution, self-assemble for 12 hours, take out Afterwards, use toluene, acetone, and deionized water to ultrasonically clean for 10 minutes in sequence, ...

Embodiment 2

[0028] Embodiment 2: Treat the surface of the material according to the following steps (alkali treatment)

[0029] (1) Material surface activation: Cut the Ti6Al4V material into discs with a diameter of 1.5cm and a thickness of 1mm. After the surface is polished to a mirror surface, it is ultrasonically cleaned with acetone, ethanol, and distilled water for 10 minutes, and immersed in 80% sodium hydroxide solution. After 6 hours of medium treatment, take it out, then wash it with a large amount of deionized water, and dry it for later use;

[0030] (2) Material surface bromination: immerse the surface-activated Ti6Al4V in 20ml of 10mM 3-bromopropyltrimethoxysilane toluene solution for self-assembly reaction for 12 hours, take it out and ultrasonically clean it with toluene, acetone, and deionized water in sequence Dry after 10 minutes for later use;

[0031] (3) Grafted anti-biopyridine molecules: Weigh 0.054 g CuBr and 3.0 g MPC respectively, dissolve them in 20 ml methanol...

Embodiment 3

[0033] Embodiment 3: according to the following steps, the material surface is processed

[0034] (1) Material surface activation: Cut the Ti6Al4V material into discs with a diameter of 1.5cm and a thickness of 1mm, polish the surface to a mirror surface, and then ultrasonically clean it with acetone, ethanol, and distilled water for 10 minutes, and put it into the vacuum chamber of the plasma processor After vacuuming, treat with oxygen plasma for 10 minutes, wash the sample with a large amount of deionized water after taking it out, and dry it for later use;

[0035] (2) Material surface bromination: first prepare a toluene solution of 3-bromopropyltrimethoxysilane with a concentration of 10mM, immerse the surface-activated Ti6Al4V disk into 20ml of silane solution, stir and react for 24 hours, take it out with toluene, Acetone and deionized water were ultrasonically cleaned for 10 minutes in sequence, then dried for later use;

[0036] (3) Grafted anti-biopyridine molecule...

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Abstract

The invention discloses a method for performing surface treatment on a material for boosting cell growth and resisting biological pollution, which comprises the following steps: firstly, performing activating treatment on a material surface, and performing surface self-assembly reaction; and then fixing amphoteric-ion molecules and polyethylene glycol molecules with the characteristics of resisting biological pollution and extracellular matrix proteins or albumen polypeptides for boosting cell growth on the material surface in turn, thereby obtaining a material with the characteristics of boosting cell growth and resisting biological pollution. By performing surface treatment on the material according to the method provided by the invention, the material surface is simultaneously endowed with the capabilities of resisting biological pollution and boosting cell growth. The method is applied to the fields of biological materials, tissue engineering, nanometer material research, and the like.

Description

technical field [0001] The invention belongs to the field of material surface modification, and in particular relates to a material surface treatment method for promoting cell growth and anti-biological pollution. Background technique [0002] After the biomaterials applied to the human body are implanted into the human body, protein or other substances usually adsorb on the surface of the material, which triggers a series of adverse biochemical reactions, resulting in the failure of the implant. Improving the anti-biofouling ability of the material surface through material surface treatment is helpful to improve the biocompatibility of the material surface; It is also necessary to have the ability to promote the growth of specific cells; for example, for artificial vascular implants, the surface of the material not only needs to have the ability to resist biofouling to inhibit the adsorption of proteins, platelets, coagulation factors, etc., but also has the ability to prom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/28
Inventor 潘长江董云肖丁红燕张兵兵
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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