Process for producing 2-keto-D-gluconic acid by high-concentration fermentation

A gluconic acid, high-concentration technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of large impurity content in yeast liquid, difficult extraction and purification, long fermentation cycle, etc., to improve the fermentation conversion rate and yield, The effect of shortening the fermentation cycle and reducing impurities

Active Publication Date: 2012-10-24
郑州拓洋生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the problems of long fermentation period, low product concentration, high impurity content of fermented yeast liquid, difficult...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A high-concentration fermentation process for producing 2-keto-D-gluconic acid. The seed culture adopts a 5L self-controlled fermenter. The formula of the seed medium is as follows. The following are mass and volume percentages: glucose 2%, corn steep liquor 0.1%, KH 2 PO 3 0.2%, urea 0.2%, MgSO 4 ·7H 2 O 0.05%, bubble enemy 0.05%; pH 6.8 before sterilization. Put the suspension of Pseudomonas fluorescens K1005 into about 3L of the above seed medium, control the stirring speed at 400r / m, the tank temperature at 33.5°C, the tank pressure at 0.04MPa, the ventilation rate at 0.5 V / V / m, and cultivate for 20h.

[0018] A 50L self-controlled fermenter was used for fermentation, and the formula of the fermentation medium was as follows: 15% glucose (40% of which was used as base material and 60% as supplementary material), 0.1% corn steep liquor, 2% calcium hydroxide, 0.05% foam enemy, and water as a solvent ; pH5.5 before sterilization. The base material glucose solution...

Embodiment 2

[0020] A high-concentration fermentative production process for 2-keto-D-gluconic acid, the seed culture is the same as in Example 1.

[0021] A 50L self-controlled fermenter is used for fermentation, and the formula of the fermentation medium is as follows: 24% glucose (40% of which is used as base material and 60% as supplementary material), 0.5% corn steep liquor, 2% calcium hydroxide, 0.05% foam enemy, and tap water as the solvent ; pH6.5 before sterilization. The base material glucose solution, corn steep liquor, and calcium hydroxide were sterilized at 120°C for 30 minutes, and mixed at 38°C, and the above-mentioned cultivated seed liquid was added to about 20L of fermentation medium to form a fermentation liquid. Glucose as feeding material was made into 11L solution, sterilized at 120°C for 30min, and added in feeding mode after cooling. After 9.5 hours of fermentation, the calcium hydroxide was exhausted, and 25% ammonia water was added to control the fermentation pH...

Embodiment 3

[0023] A high-concentration fermentative production process for 2-keto-D-gluconic acid, the seed culture is the same as in Example 1, and the strain is Arthrobacter globosa K1022.

[0024] A 50L self-controlled fermenter was used for fermentation, and the formula of the fermentation medium was as follows: 32% glucose (40% as base material and 60% as supplementary material), 1% corn steep liquor, 3% calcium hydroxide, 0.06% foam enemy, and water as the solvent ; pH7.0 before sterilization. The bottom material glucose solution, corn steep liquor, and calcium hydroxide were sterilized at 120°C for 30 minutes, and mixed at 38°C, and the above-mentioned cultured seed liquid was added to about 20L of fermentation medium. Glucose as supplementary material was made into 12L solution, sterilized at 120°C for 30min, and added by fed-batch method after cooling. After 10 hours of fermentation, the calcium hydroxide was exhausted, and 30% ammonia water was added to control the fermentatio...

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Abstract

The invention discloses a process for producing 2-keto-D-gluconic acid by high-concentration fermentation. A microorganism for converting glucose into the 2-keto-D-gluconic acid or salt of the 2-keto-D-gluconic acid is cultured in a fermentation container, as a carbon source in a fermentation culture medium, the glucose is subjected to fermentation culture, and the pH (Potential Of Hydrogen) value before sterilization is 5.5-7.0; and the fermentation culture medium consists of the following substances by massvolume: 10-32% of glucose, 0.1-1% of maize pulp, 1-5% of calcium hydroxide, 0.02-0.08% of de-foaming agent and a solvent as water. The pH value is adjusted by calcium hydroxide in the beginning of fermentation so that the cost is lower, the pH value of a fermentation solution is more accurately adjusted and controlled by liquid ammonia or ammonia water in the mid- to late-period of fermentation so that the fermentation solution is kept in the environment for optimal growth and acid production of the microorganism, and a nitrogen source is supplied at the same time, so that the acid production rate and the acid production concentration of bacteria are increased, the fermentation conversion rate and the yield are increased, the fermentation cycle is shortened, the quality of the fermentation liquid is high, the amount of the impurity is small, the extracting and purifying processes are convenient, and the emission of calcium sulfate waste residues is reduced at the same time; and the formed byproduct is ammonium sulfate which can be used as a fertilizer.

Description

technical field [0001] The invention belongs to the field of preparation of salt synthetic precursor 2-keto-D-gluconic acid of food additives, in particular to a process for preparing 2-keto-D-gluconic acid or its salt by high-concentration fermentation. Background technique [0002] 2-Keto-D-gluconic acid is one of the organic acids produced on a large scale in the industry at present, and it is the precursor for the synthesis of food antioxidant sodium D-erythorbate and D-erythorbic acid. The production methods of 2-keto-D-gluconic acid mainly include enzymatic method, chemical synthesis method and fermentation method. Due to factors such as production costs, the indirect fermentation technology of bacterial fermentation is mostly used in industrial production at present. [0003] At present, in industrial production, 2-keto-D-gluconic acid is produced by fermentation, using D-glucose as raw material. The method has the following disadvantages: due to the feedback inhibi...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12R1/39C12R1/06
Inventor 闫世梁黄惠英崔凤霞秦天仓史小莉肖媛张建国朱腾跃王敬臣宋向军王新华刘银霞张生克
Owner 郑州拓洋生物工程有限公司
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