Electrode loaded with multi-layer glucose oxidase magnetic nanospheres and preparation method of electrode

Abstract

The invention discloses a preparation method of an electrode loaded with glucose oxidase magnetic nanospheres. The preparation method comprises the following steps of: preparing the nanospheres and 25 percent ethanol solution into mixed solution with molar ratio of 1:20-1:30 and electromagnetically stirring at room temperature until complete dissolution; transferring a dissolution system into an ice water bath; adding a proper amount of 5-10 percent analytically pure ammonia water, continuously stirring for 0.5-1 hour and aging the colloid at normal temperature for 1-2 hours to prepare nanoparticle sol; taking an electrode substrate, coating the prepared nanoparticle sol on the electrode substrate by using a pulling method at the pulling speed of 6-8 cm / min and standing to prepare a coated substrate; preparing the nanoparticles, toluene solution and water into mixed solution according to a certain molar ratio; vertically soaking the prepared coated substrate in a reactor with mixed solution; applying a horizontal magnetic field to the reactor; and reacting under the magnetic field condition of 5,000-10,000 gausses for 0.5-1 hour to prepare the electrode loaded with the multi-layer nanospheres with enzyme.

Description

technical field [0001] The invention relates to a method for preparing an electrode, in particular to a method for preparing an electrode loaded with multilayer glucose oxidase magnetic nano microspheres. Background technique [0002] Such as Figure 4 Shown: It reflects the basic process of converting the blood sugar detection process from a chemical reaction to an electrical signal. Under the catalysis of chemical decomposition reaction, charged particles (such as hydroxide ions, hydrogen ions and electrons) are released. The charged particles are transmitted to the electrical signal detection system through the electrodes and the wires connected to the electrodes. According to the generated current The size judges the intensity of the reaction, and the greater the intensity of the reaction, the more severe the reaction and the higher the glucose content; and vice versa. [0003] In the prior art, the enzyme layer mainly uses the method of directly coating the reaction so...

AI Technical Summary

Problems solved by technology

[0005] Glucose-reactive enzymes are difficult to form a stacked structure on the electrode surface, that is, only a small amount of reaction enzymes exist at a certain reaction point, and they are easily consumed in the glucose decomposition reaction. In the concentration zone, glucose molecules will permeate into this reaction zone, but because the reaction enzyme is not compensable, the point no longer has the reaction enzyme, so the reaction has no continuity

The resulting current is small and lasts for a short time, making it difficult to detect and judge

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