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Method for detecting seven aromatic amine compounds in human urine through liquid chromatography-series mass spectrometry

A technology of tandem mass spectrometry and liquid chromatography, which is applied in the field of detection of seven aromatic amine compounds in human urine by liquid chromatography-tandem mass spectrometry, can solve cumbersome and time-consuming problems, and achieve simplified sample pretreatment steps, high sensitivity, and improved Analyzing the Effects on Efficiency

Inactive Publication Date: 2012-11-21
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a liquid chromatography-tandem mass spectrometry method for the cumbersome and time-consuming pretreatment of the determination method of aromatic amine compounds in human urine in the prior art

Method used

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  • Method for detecting seven aromatic amine compounds in human urine through liquid chromatography-series mass spectrometry
  • Method for detecting seven aromatic amine compounds in human urine through liquid chromatography-series mass spectrometry
  • Method for detecting seven aromatic amine compounds in human urine through liquid chromatography-series mass spectrometry

Examples

Experimental program
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Effect test

Embodiment 1

[0038] will be -18 o Urine samples stored in C were removed and thawed at room temperature. Take 5 mL of the thawed urine sample and place it in a 50 mL plastic centrifuge tube, add 1 mL of concentrated hydrochloric acid solution, 80 oC water bath for 1 h. After the sample was cooled, about 1.2 mL of 10 mol / L NaOH solution was added (to neutralize the sample solution to a pH of about 7.0), followed by 10 mL of ammonium acetate-ammonia buffer solution (0.5 mol / L, pH 7.0). Add 50 μL D7-1-aminonaphthalene and D5-aniline mixed internal standard solution. The samples were purified using a PAH solid-phase extraction column (Supel MIP& reg; pk of 50, 3 mL) produced by Shanghai Anpu Company. The sample solution was loaded onto a PAH molecular imprinting column activated with 1 mL cyclohexane, washed with 1 mL cyclohexane, and finally eluted with 10 mL ethyl acetate. The eluate was blown dry with nitrogen, reconstituted by adding 100 μL of methanol solution, and analyzed by LC-MS / M...

Embodiment 2

[0040] Randomly select 10 urine samples, take 100 mL each after thawing at room temperature and mix well. Take 5 mL of mixed urine sample, perform sample pretreatment according to the steps described in Example 1, and perform LC-MS / MS analysis. Five parallel experiments were carried out on the same day, and the relative standard deviation of each compound was between 5.36% and 7.87%, indicating that the method has good reproducibility and stability.

[0041]

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Abstract

The invention provides a method for detecting seven aromatic amine compounds in human urine through a liquid chromatography-series mass spectrometry. The method is to detect aniline, o-toluidine, m-toluidine, 1-naphthylamine, 2-naphthylamine, 3-aminobphenyl and 4-aminobphenyl, and is characterized by comprising the steps of hydrolyzing urine via concentrated HCl, purifying the sample through PAH (Polycyclic Aromatic Hydrocarbon) molecular imprinting columns, and analyzing through the liquid chromatography-series mass spectrometry (LC-MS / MS). Compared with the prior art, the method has the advantages that the LC-MS / MS method is adopted, so that higher sensitivity is achieved, and the pre-processing of the sample can be greatly simplified (about 2 hours are consumed); the pre-processing through a GC-MS (Gas Chromatography-Mass Spectrometer) method as shown in a literature is complicated and wastes time (by more than 6 hours). The method provided by the invention can effectively improve the analyzing efficiency, and is relatively suitable for analyzing seven aromatic amine compounds in human urine.

Description

[0001] technical field [0002] The present invention belongs to the research of cigarette smoke exposure biomarkers, and specifically relates to a liquid chromatography-tandem mass spectrometry method for detecting seven kinds of aromatic amine compounds in human urine, namely aniline, o-toluidine, m-toluidine, 1- Detection of aminonaphthalene, 2-aminonaphthalene, 3-aminobiphenyl and 4-aminobiphenyl. [0003] Background technique [0004] Aromatic amine compounds are widely present in the ambient atmosphere, dyes, drugs, food additives, pesticides, plastics, rubber, cosmetics, discharged wastewater, tobacco and smoke. Aromatic amine compounds are generally toxic and can enter the human body through inhalation or skin contact. Some aromatic amine compounds are also carcinogenic. People who are exposed to dyes such as naphthylamine are high-risk groups for bladder cancer and leukemia. [1] . Cigarette smoke contains a variety of carcinogenic aromatic amines, such as 1-amin...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 余晶晶王昇赵阁王冰刘克建潘立宁张晓兵谢复炜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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