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Preparation and application of composite structure microsphere with color change ability

A technology of composite microspheres and polystyrene microspheres, which is applied in the preparation of microspheres, medical preparations of non-active ingredients, microcapsule preparations, etc., can solve problems such as complex operation, weak electrostatic adsorption force, and easy breakage. Achieve the effects of strong anti-interference, easy separation and low price

Active Publication Date: 2014-09-24
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the LB / LS membrane has problems such as complicated operation and inconvenient application. The vesicle solution is not easy to separate and breaks easily, and the fiber is not suitable for the field of biomolecular recognition. These drawbacks seriously restrict the development of PDA biosensors. How to make PDA sensors It is portable, stable, and has a wide range of recognition functions, which has become a research hotspot
[0004] It has been reported in the literature that the PDA vesicle solution is attached to the surface of the glass substrate by layer-by-layer assembly, thereby increasing its storage capacity and portability. However, the interference of the glass itself has a relatively large impact on recognition. At the same time, the fixed PDA sensors are not easy to effectively combine with biomolecules, which limits their recognition range and ability
In order to increase the portability of the sensor, some people have studied the use of charge adsorption to adsorb PDA on the surface of silica balls. Although qualitative biometric identification can be performed, due to the weak pure electrostatic adsorption force and the problem of adsorption saturation, How to improve the photopolymerization performance of PDA, so as to improve its detection performance has become a difficult point

Method used

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  • Preparation and application of composite structure microsphere with color change ability
  • Preparation and application of composite structure microsphere with color change ability
  • Preparation and application of composite structure microsphere with color change ability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the preparation of diacetylene vesicle solution

[0048] 1. Purification of diacetylene (PCDA)

[0049] The powder of diacetylene (PCDA) molecule was dissolved in chloroform solution, and the polymer was removed through filter paper, and the ELELA N-1100 The chloroform solvent was completely spin-dried in a vacuum rotary evaporator at 45° C. to obtain a white solid powder. Protect from light, dry in vacuum at low temperature, and store for later use.

[0050] 2. Preparation of diacetylene vesicle solution

[0051] Weigh 1.87 mg of purified diacetylene (PCDA) monomer powder and add it to 10 mL of phosphate buffer solution (pH=7.4), and use a SCIENIZ JY92-II ultrasonic cell disruptor to sonicate for 5 minutes to obtain a clear transparent solution. The concentration is 0.5 mM. The prepared solution was kept at 4° C. overnight in the dark using tin foil. The next day, it was filtered through a membrane with a pore size of 0.2 μm-0.5 μm and stored for lat...

Embodiment 2

[0052] Embodiment 2, utilize molecular glue to prepare PSPCDA composite microsphere material

[0053] Centrifuge and wash the polystyrene microspheres (with a particle size of about 1 μm) to be used under a centrifugal force of 4000 g (the surface of the purchased polystyrene microspheres is modified with sulfonic acid groups and is negatively charged, and the Zeta potential is -28.6 mV), and take 3.4 mL of mass The fraction is 0.134% polystyrene microsphere solution (approximately 10 10 1), use 1M HCl standard solution to adjust the pH value of the solution between 3-3.2, add 1mL, 1mg / mL polyethyleneimine (PEI) (Mw 50000) solution (concentration containing NaCl is 0.1M), Let stand and incubate for 20min. Under the centrifugal force of 4000g-8000g, centrifuge to remove excess PEI, and add an equal volume of deionized water. The centrifugation and washing were repeated three times. At this time, the PSPEI microsphere solution was positively charged, and the Zeta potential was...

Embodiment 3

[0057] Embodiment 3, utilize molecular glue to prepare PSPCDA composite material

[0058] Centrifuge and wash the polystyrene microspheres (with a particle size of about 1 μm) to be used under a centrifugal force of 4000 g (the surface of the purchased polystyrene microspheres is modified with sulfonic acid groups and is negatively charged, and the Zeta potential is -28.6 mV), and take 3.4 mL of mass The fraction is 0.134% polystyrene microsphere solution (approximately 10 10 ), use 1M HCl standard solution to adjust the pH value of the solution to 7.5, add 1 mL of 1 mg / ml polyallylamine hydrochloride (PAH) (Mw 70000) solution (concentration containing NaCl is 0.3M), let stand Incubation for 20min. Under the centrifugal force of 4000g-8000g, centrifuge to remove excess PAH, and add an equal volume of deionized water. Repeat three times. At this time, the PSPAH microsphere solution is positively charged, and the Zeta potential is about 15.2. Adjust the pH value of the soluti...

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Abstract

The invention discloses a composite microsphere with a biosensing effect and its preparation method. The preparation method of the composite microsphere comprises: using polyelectrolyte as a molecular glue, by means of static electricity and hydrophobic interaction, assembling diacetylene vesicles on the surface of a polystyrene microsphere, thus forming a composite microsphere particle; and subjecting the composite microsphere particle to ultraviolet irradiation, thus obtaining the polystyrene / polydiacetylene vesicle composite microsphere with color change and biomolecule recognition abilities. The microsphere utilizes the polyelectrolyte as the molecular glue to make diacetylene adsorbed onto the microsphere surface. Compared with other forms of diacetylene sensors, the microsphere provided in the invention has the advantages of stability, easy storage, easy separation, and strong anti-interference property. Compared with enzyme-linked immunosorbent assay and other immunodetection methods, the microsphere also has the characteristics of simplicity, rapidity, visibility to naked eyes, and low price, etc. At the same time, the composite microsphere can also be used in micro-reactors and used for separation, drug carriers as well as various aspects.

Description

technical field [0001] The invention relates to the preparation and application of a composite structure microsphere with color changing ability. Background technique [0002] Polydiacetylene (PDA) biosensors have received widespread attention since their appearance. Polydiacetylene has a unique conjugated structure, which can cause the transition of delocalized π electrons under ultraviolet light, and photopolymerization occurs, showing a bright blue color. When the blue polydiacetylene skeleton is disturbed by external stimuli, the polymer The conformation of the backbone will change, resulting in a blue shift of its absorption peak, turning the color of polydiacetylene into red, and producing unique fluorescence characteristics, thereby realizing the detection of stimuli. PDA biosensors have recognition functions for solvents, pH values, temperatures, and biomolecules (sugar-proteins, antigen-antibodies, DNA, etc.), can quickly sense external stimuli, generate visible re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569B01J13/02A61K47/32
Inventor 江龙董文杰
Owner INST OF CHEM CHINESE ACAD OF SCI