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Method for synchronously extracting and separating icariin and icarisid II from herba epimedii

A technology of icariin and icariin, applied to medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc. Tissue damage and other issues, to avoid health hazards, simplify the extraction and separation process, and reduce pollution

Inactive Publication Date: 2012-12-19
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent application method effectively improves the purity of icariin and makes the separation and purification easier in the later stage. However, in this method, dichloromethane is used, which is a toxic solvent, and it will damage the human liver at high concentrations. , kidney and brain tissue damage, and it has carcinogenic effects on animals, is a potential human carcinogen
For icariin II, at present, it is mostly obtained by hydrolyzing icariin, and there is no report on the effective purification of icariin II from Epimedium, although this method can effectively purify Epimedium glycoside II, but this method has hydrolyzed icariin, and icariin cannot be obtained at the same time, which is not conducive to the utilization of medicinal material resources
[0006] At present, there is no relevant report on the direct separation and extraction of icariin and icariin II using the same separation method

Method used

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  • Method for synchronously extracting and separating icariin and icarisid II from herba epimedii
  • Method for synchronously extracting and separating icariin and icarisid II from herba epimedii
  • Method for synchronously extracting and separating icariin and icarisid II from herba epimedii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 The separation method of icariin and icariin II of the present invention

[0046] 1) Take 500g of Korean Epimedium (Epimedium koresnum Nakai.) and at the same time: measure icariin and moisture.

[0047] 2) Soak the medicinal materials mentioned in 1) with 17 times the amount of water for 15 hours, decoct for 2 hours, and filter to obtain medicinal residues and filtrate respectively.

[0048] 3) Soak the medicinal residue obtained in 2) with 10 times the amount of water for 15 hours, decoct for 2 hours, and filter to obtain the second medicinal residue and filtrate respectively.

[0049] 4) Combine the filtrates obtained in 2) and 3), and discard the dregs of the two times. The combined filtrates were left to stand for 9 hours, and the supernatant was rotary evaporated at 55°C-70°C under reduced pressure, concentrated to 500ml, and the relative density was measured at 60°C to be in the range of 1.1-1.2 to obtain the extract.

[0050] 5) Add 95% ethanol to...

Embodiment 2

[0065] Embodiment 2 The separation method of icariin and icariin II of the present invention

[0066] 1) Take 500g of Epimedium brevicornu Maxim. At the same time: measure icariin and moisture.

[0067] 2) Soak the medicinal materials mentioned in 1) with 19 times the amount of water for 15 hours, decoct for 2 hours, and filter to obtain medicinal residues and filtrate respectively.

[0068] 3) Soak the dregs obtained in 2) with 11 times the amount of water for 15 hours, decoct for 2 hours, and filter to obtain the second dregs and filtrate respectively.

[0069] 4) Combine the filtrates obtained in 2) and 3), and discard the dregs of the two times. The combined filtrates were left to stand for 13 hours, and the supernatant was rotary evaporated at 55°C-70°C under reduced pressure, concentrated to 500ml, and the relative density was measured at 60°C in the range of 1.1-1.2 to obtain the extract.

[0070] 5) Add 95% ethanol to the extract obtained in 4) until the ethanol conc...

Embodiment 3

[0079] Embodiment 3 The separation method of icariin and icariin II of the present invention

[0080] 1) Take 500g of Epimedium sagittatum (Sieb. et Zucc.) Maxim. and at the same time: measure icariin and moisture.

[0081] 2) Soak the medicinal materials mentioned in 1) with 19 times the amount of water for 15 hours, decoct for 2 hours, and filter to obtain medicinal residues and filtrate respectively.

[0082] 3) Soak the medicinal residue obtained in 2) with 7 times the amount of water for 15 hours, decoct for 2 hours, and filter to obtain the second medicinal residue and filtrate respectively.

[0083] 4) Combine the filtrates obtained in 2) and 3), and discard the dregs of the two times. The combined filtrates were left to stand for 11 hours, and the supernatant was rotary evaporated under reduced pressure at 55°C-70°C, concentrated to 500ml, and the relative density was measured at 60°C to be in the range of 1.1-1.2 to obtain the extract.

[0084] 5) Add 95% ethanol to...

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Abstract

The invention provides a method for synchronously extracting and separating icariin and icarisid II from herba epimedii. The method obtains high-purity extract of icariin and icarisid II through macroporous adsorption resin gradient elution respectively. The invention also provides a method for extracting and separating icariin or icarisid II. The method provided by the invention not only realizes synchronous extraction and separation of icariin and icarisid II and simplifies the technological process, but also obtains a medicinal intermediate with relatively high purity, obviously increases the yield of monomer components, improves the utilization rate of medicinal materials and reduces the production cost; and meanwhile, without using organic solvents with relatively high toxicity, the method guarantees the safety of operators, realizes the green production and has great industrial application prospects.

Description

technical field [0001] The invention relates to a method for simultaneously extracting and separating icariin and icariside II from epimedium. Background technique [0002] Epimedium is a traditional tonic Chinese medicine, which has multiple functions such as tonifying kidney yang, strengthening muscles and bones, and dispelling rheumatism. Due to the development of modern natural medicinal chemistry, there are more and more studies on the monomeric active ingredients in Epimedium. [0003] Icariin, one of the isolated monomers extracted from Epimedium, has positive curative effects on improving the cardiovascular system, regulating endocrine, enhancing immunity, sex hormone-like effects, anti-tumor, and anti-hepatic toxicity. . Icariside II, a new monomer developed from Epimedium in recent years, is superior to icariin in improving the function of vascular endothelial cells; in inhibiting lipid peroxidation, total antioxidant capacity and In terms of reducing power, the...

Claims

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Application Information

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IPC IPC(8): A61K36/296A61K31/7048A61P39/06A61P19/10A61P37/02A61P35/00A61P9/00
Inventor 曾锐刘海萍瞿燕韦涛
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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