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Steroid enol compound as well as preparation method thereof and application thereof

A compound and sterenol technology, applied in the field of phytochemistry, can solve the problems of drug residues, poor control effect, pesticide pollution, etc., and achieve the effects of low cost and simple preparation method

Inactive Publication Date: 2012-12-26
SHAANXI SUNGER ROAD BIO SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current situation of domestic viral pesticides is: (1) Due to the absolute parasitism of the virus on the host (specialized parasitism), the energy and materials required for its replication are provided by the host cell. Although there are many mixed preparations, there is a lack of direct action on the virus. Single-compound pesticide varieties, resulting in poor control effect
(2) At present, the vast majority of drugs registered in China use morpholine hydrochloride as the main ingredient, and morpholine hydrochloride has long been used in the treatment of clinical viral diseases. If it is used for a long time in food crops, fruits and vegetables, etc., it will cause Drug residues, long-term consumption of food with residues will usually cause drug resistance problems in the human body
(3) Long-term and large-scale application of chemical virus preparations will cause serious "pesticide public hazards"
At present, there are few technical reports on the development and research of the antiviral application of Malvaceae plants. If the development and research of biologically active sterenol compounds from Malvaceae plants is successful, it will be beneficial to pesticides, especially antiviral related research. domain is of great significance

Method used

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  • Steroid enol compound as well as preparation method thereof and application thereof
  • Steroid enol compound as well as preparation method thereof and application thereof
  • Steroid enol compound as well as preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Preparation of the compound of formula (II) of the present invention

[0026] (1) Collect the branches, leaves and bark of Malvaceae plants, dry them and crush them;

[0027] (2) Weigh the raw materials, add 1 liter of water for every kilogram of crushed plant raw materials, fully seal and soak;

[0028] (3) Add 3 liters of methanol solvent per kilogram of raw materials, fully seal and stir until completely dissolved, and concentrate under reduced pressure to obtain the crude extract of plant raw materials;

[0029] (4) Put 30 grams of the crude extract of plant raw materials into an extraction tank, add an equal volume of water, and ultrasonically crush and dissolve at room temperature. After suction filtration, extract three times with petroleum ether, ethyl acetate, and n-butanol respectively. Obtain petroleum ether phase, ethyl acetate phase and n-butanol phase; Then select ethyl acetate phase and n-butanol phase for column separation;

[0030] (5) M...

Embodiment 2

[0037] Example 2: The control effect of the compound of formula (II) of the present invention on zucchini mosaic virus and watermelon mosaic virus

[0038] (1) Preparation of seedlings for inoculation: Zucchini and watermelon seeds are first soaked in warm soup for 30 minutes, and then germinated in a petri dish. When the buds grow to about 1cm, select consistent buds and sow them in flowerpots, and put them on insect-proof nets. Cultivate in a greenhouse, and inoculate and spray when it grows to two cotyledons and one heart leaf.

[0039] (2) Treatment method: The test is divided into 4 treatments, each with 6 strains, with disease control (inoculation only, no spraying), blank control (no vaccination, no spraying), spraying treatment (inoculation first, After 24 hours, the compound of formula (II) and the control drug (20% copper morpholino hydrochloride wettable powder) were sprayed, and observation and sampling were carried out on the 7th day after spraying;

[0040] (3) ...

Embodiment 3

[0048] Example 3: Field control effect of the compound of formula (II) of the present invention on tobacco mosaic virus and wheat yellow dwarf virus

[0049] 1. Experimental conditions: Tobacco and wheat were selected as crops; the targets were tobacco mosaic virus and wheat yellow dwarf virus.

[0050] 2. Experimental design and arrangement: The experimental drug is the antiviral preparation of this study, and 20% morpholinidine hydrochloride wettable powder control drug and water control are set up. A total of 3 treatments, repeated 3 times, a total of 9 plots. The experimental plots were arranged in random blocks.

[0051] 3. Application method: Spray the pesticide 20 days after transplanting, and spray the pesticide for the second time after every 7 days.

[0052] 4. Experimental results:

[0053] The control effect of table 3 test medicament to tobacco mosaic virus disease

[0054]

[0055] The control effect of table 4 test pesticides on wheat yellow dwarf virus d...

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Abstract

The invention discloses a novel steroid enol compound illustrated by a formula (II), a preparation method of the compound and a purpose of the compound in prevention and treatment of plant virus disease.

Description

technical field [0001] The invention relates to the technical field of phytochemistry, in particular to a new class of sterol compounds and their preparation method and application, especially for the application of antiviral agents. Background technique [0002] Plant viral diseases pose a serious threat to crop yield and quality worldwide, causing a 10-30% reduction in yield and an economic loss of about 20 billion U.S. dollars each year. my country is a large agricultural country, and the economic losses caused by viral diseases to food production, horticultural crops, and cash crops are about tens of billions of yuan every year. Therefore, there is an urgent need to develop antiviral drugs. Foreign countries have increased research in this area since the early 1960s. In 1985, the alginic acid casein (Mosanon) registered in Japan was extracted from Mirabilis jasmine and Lentemin (Lentemin), an extract of mushroom juice, was used to prevent and treat tomato viruses. sick...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00A01N45/00A01P1/00
Inventor 吴云锋
Owner SHAANXI SUNGER ROAD BIO SCI