Preparation method of egg yolk antibody for treating multocida pasteurellosis

Abstract

The invention discloses a preparation method of an egg yolk antibody for treating multocida pasteurellosis. The preparation method is characterized by including the steps: (1) preparing a pasteurella multocida antigen; (2) immunizing an egg laying hen; and (3) extracting the egg yolk antibody. The preparation method has the advantages that combination of an acidified water extraction method with an octylic acid extraction method is preferable for preparing a pasteurella multocida vaccine, the preparation method is free from environmental pollution, and the extracted product is safe and effective to animals; the egg yolk antibody vaccine enables the egg yolk antibody to be directly acted on a hen body, and homology and effectiveness of the egg yolk antibody are higher than those of other biological agents; and the problem of residues of antibiotic drugs on meat, poultry and egg products is solved, production cost is low, and the quality of the egg yolk antibody is effectively improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing egg yolk antibody for treating Pasteurella multocida. Background technique [0002] Pasteurellosis is an acute, febrile infectious disease caused by Pasteurella multocida. The acute type of pasturellosis in animals is often characterized by sepsis and hemorrhagic inflammation, so it was also called "hemorrhagic sepsis" in the past; the chronic type often manifests as purulent lesions in subcutaneous connective tissue, joints and various organs, and often Mixed infection or secondary with other diseases. Such diseases can occur throughout the year, causing damage throughout the entire breeding cycle, and are prone to cross-infection. If it is not effectively controlled, it will cause serious economic losses to the poultry industry. [0003] When the flocks get sick, treatment measures should be taken immediately, and where conditions permit, effective drugs s...

AI Technical Summary

Problems solved by technology

For areas where it often occurs or chicken farms, the effect of drug treatment is decreasing day by day, and it is difficult to effectively control the disease, but it directly leads to antibiotic residues, hormone residues, heavy metal residues and pesticide residues in meat and poultry food far exceeding my country's custom-made comprehensive color pollution-free Standards and international export standards; some breeding enterprises use traditional Chinese medicine preparations to prevent and control such diseases in chickens, but due to various reasons, the effect of traditional Chinese medicine poultry medicines is not satisfactory, the main reason is that poultry absorb Chinese medicines No, the efficacy of the medicine cannot be well reflected in the chicken body, so the disease cannot be fundamentally controlled in the chicken flock