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Method for degrading enteromorpha polysaccharide

A technology for the degradation reaction of E. enterica polysaccharide, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problems of uneven degradation products, polysaccharide structure damage, slow polysaccharide rate, etc., and the time required to achieve is short , lower molecular weight, fast reaction effect

Active Publication Date: 2013-01-30
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method mainly involves acidolysis and oxidative degradation. A large amount of volatile strong acid is used in acidolysis, which causes environmental pollution and the degradation products are uneven; the oxidative degradation method is a low-cost and environmentally friendly method, but hydrogen peroxide is used alone Degradation rate of seaweed polysaccharide is slow, and high concentration of hydrogen peroxide will cause destruction of polysaccharide structure

Method used

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  • Method for degrading enteromorpha polysaccharide
  • Method for degrading enteromorpha polysaccharide
  • Method for degrading enteromorpha polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Dissolve 0.5g of high-molecular-weight Enteromorpha polysaccharide (>1500KDa) in 50ml of distilled water, add 4.2ml of concentrated hydrochloric acid and 1.5ml of 30% (mass fraction) hydrogen peroxide to the Enteromorpha polysaccharide solution, and then microwave at 70 ℃, degraded for 5-60min, according to different degradation times to obtain low-molecular-weight Enteromorpha polysaccharides and oligosaccharides; the molecular weights of different low-molecular-weight Enteromorpha polysaccharides and oligosaccharides obtained were determined by gel exclusion chromatography (see figure 1 ).

[0026] At the same time, dissolve 0.5g of high-molecular-weight Enteromorpha polysaccharide (>1500KDa) in 50ml of distilled water, add 4.2ml of concentrated hydrochloric acid and 1.5ml of 30% (mass fraction) hydrogen peroxide to the solution of Enteromorpha polysaccharide, and then adopt the traditional heating method to process Enteromorpha For the degradation of polysaccharides,...

Embodiment 2

[0029] Dissolve 0.5g of high-molecular-weight Enteromorpha polysaccharide (>1500KDa) in 50ml of distilled water, add 4.2ml of concentrated hydrochloric acid and 1.5ml of hydrogen peroxide with a mass fraction of 30% to the solution of Enteromorpha polysaccharide, and degrade it for 15 minutes at a microwave power of 600W and a reaction temperature of 60°C After the reaction, the solution was neutralized to neutral with NaOH (2mol / L), concentrated by dialysis, adding 4 times the volume of 95% ethanol for precipitation, and then centrifuged at 3000r / min to collect the precipitate, freeze-dried to obtain low Molecular weight of Enteromorpha polysaccharides. The obtained product utilizes gel exclusion chromatography to measure its molecular weight to be 247.0KDa ( figure 2 shown), its chemical composition is shown in Table 1, and the infrared spectrum of the degradation product is compared with the infrared spectrum of the raw material Enteromorpha polysaccharide ( image 3 show...

Embodiment 3

[0031] Dissolve 0.5g of high-molecular-weight Enteromorpha polysaccharide (>1500KDa) in 50ml of distilled water, add 4.2ml of concentrated hydrochloric acid and 1.5ml of 30% hydrogen peroxide to the Enteromorpha polysaccharide solution, and degrade it at a microwave power of 600W and a reaction temperature of 70°C After 30 minutes, neutralize the reacted solution with lye NaOH (2mol / L) to neutrality, concentrate by dialysis, add 4 times the volume of the concentrate to precipitate with 95% ethanol, then centrifuge at a speed of 3000r / min to collect the precipitate, and freeze-dry it. Enteromorpha polysaccharides with low molecular weight were obtained. The resulting product was determined to have a molecular weight of 19.02KDa ( figure 2 shown), its chemical composition is shown in Table 1, and the infrared spectrum of the degradation product is compared with the infrared spectrum of the original Enteromorpha polysaccharide raw material ( image 3 Shown) found the characteri...

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Abstract

The invention belongs to a marine chemical engineering technology, in particular to a method for degrading enteromorpha polysaccharide, which concretely comprises the steps of: adding hydrochloric acid and hydrogen peroxide into enteromorpha polysaccharide solution, and carrying out degradation reaction under the assistance of microwave to obtain the enteromorpha polysaccharide or oligosaccharide with the low molecular weight of 3-400K Da. Compared with the traditional heating reaction, the method can be used for preparing the enteromorpha polysaccharide with the low molecular weight and greatly shortens the action time; and the obtained enteromorpha polysaccharide or oligosaccharide has no obvious difference in the aspect of basic chemical structure compared with the initially prepared enteromorpha polysaccharide raw material.

Description

technical field [0001] The invention belongs to marine chemical engineering technology, and in particular relates to a method for degrading enteromorpha polysaccharide with the aid of microwave temperature control and hydrochloric acid and hydrogen peroxide. Background technique [0002] Enteromorpha polysaccharide is a water-soluble sulfated heteropolysaccharide derived from the marine macroalgae Enteromorpha, which consists of xyloglucan, glucuronoglycan and cellulose linked by linear β-1,4 glycosidic bonds. Enteromorpha is industrially used in papermaking and biogas production based on the properties of its polysaccharides. Enteromorpha polysaccharide also has many physiological functions: lowering blood fat, increasing superoxide dismutase (SOD) activity, reducing lipid peroxidation (LPO), anti-inflammatory, inhibiting skin cancer, etc., and can be used to produce dietary fiber. Enteromorpha polysaccharides are simple to prepare, safe and non-toxic, and have great appli...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H3/06C07H1/00
Inventor 李鹏程李冰刘松邢荣娥于华华李克成王雪芹秦玉坤李荣锋魏振华
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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