Acetylxylan esterase and application thereof

An acetylxylan ester and deacylating technology, applied in the field of acetylxylan esterase and its application, can solve the problems of poor activity and performance, and limit wide application
CN102952787BActive Publication Date: 2014-07-16NANJING TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING TECH UNIV
Publication Date
2014-07-16

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Abstract

The invention discloses a novel DNA of acetyl xylan esterase, the nucleotide sequence of which is shown in SEQ ID NO:1. Its amino acid sequence is shown in SEQ ID NO:2. The highest activity occurs when the protein structure is a homohexamer. The invention also discloses the cloning vector and cloning technology containing the above-mentioned novel acetylxylan esterase DNA, and the application of recombinant esterase to the hydrolysis of short-chain fatty acid lipids and acyl sugars and its important role in the production of β-lactam antibiotics. The application of the intermediate-deacylated 7-aminocephalosporanic acid and deacylated cephalosporin C has important industrial application value.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, in particular to a Bacillus subtilis ) obtained in the new esterase and expression vector, recombinant esterase and the important intermediate of recombinant esterase in the hydrolysis of short-chain fatty acid lipids, deacylation reaction and β-lactam antibiotics - deacylated 7-aminocephalosporin Application of alkanoic acid and deacylated cephalosporin C. Background technique

[0002] Acetyl xylan esterase (AXE for short) is a class of esterases that can deacylate the acylated xylopyranose in xylan or xylooligosaccharides and release acetic acid, classified in carboxyl Esterase family 7, which can hydrolyze short-chain fatty acids and various acylated compounds, has broad substrate specificity.

[0003] Nowadays, acetyl xylan esterase has shown great scientific research value and industrial application value, which are mainly reflected in the following two aspects. On the one ha...

Claims

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