Method for preparing dual-function targeting quantum dot lipidosome

A quantum dot and dual-function technology, applied in the field of nanomaterials, can solve problems such as difficult quality control, unknown biological safety, complex formulation and preparation process of active targeting preparations, and achieve good targeting and high encapsulation efficiency. Effect

Inactive Publication Date: 2013-03-20
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with passive targeting, the active targeting nano drug delivery system shows better targeting and growth inhibition on tumor tissue, however, due to the complex formulation and preparation process of active targeting preparations, quality control is difficult, unknown Issues such as biological safety and so far only a small number of products have entered Phase I or Phase II clinical trials

Method used

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  • Method for preparing dual-function targeting quantum dot lipidosome
  • Method for preparing dual-function targeting quantum dot lipidosome

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The liposome membrane material modified by RPARPAR is formulated as hydrogenated soybean lecithin / cholesterol / methoxypolyethylene glycol-distearoylphosphatidylethanolamine complex / RPARPAR-PEG-DSPE (60:40:2:0.5, mol / mol). Weigh the above membrane material and dissolve it in chloroform, and remove the organic solvent by rotary evaporation to obtain a uniform lipid membrane. Add CdTe / CdS aqueous solution for hydration, and shake in a water bath at 60°C for 2 hours to obtain a liposome suspension. In a water bath at 60 °C, use a high-pressure homogenizer to sequentially squeeze the liposomes through 400, 200, and 100 nuclear pore membranes to reduce their particle size. Then use physiological saline as eluent to separate and remove unencapsulated quantum dots through Sephadex G-50 chromatography column to obtain active targeting liposomes containing quantum dots. Add doxorubicin normal saline solution, 60 ℃ water bath for 20 min. Elute with normal saline and pass throug...

Embodiment 2

[0025] The liposome membrane material modified by RPARPAR was formulated as dipalmitoylphosphatidic acid / cholesterol / methoxypolyethylene glycol-distearoylphosphatidylethanolamine complex / RPARPAR-PEG-DSPE (90:10:2:0.5, mol / mol). Weigh the above membrane material and dissolve it in chloroform, and remove the organic solvent by rotary evaporation to obtain a uniform lipid membrane. Add CdTe / CdS / ZnS aqueous solution for hydration, and shake in a water bath at 60°C for 2 hours to obtain a liposome suspension. In a water bath at 60 °C, use a high-pressure homogenizer to sequentially squeeze the liposomes through 400, 200, and 100 nuclear pore membranes to reduce their particle size. Then use physiological saline as eluent to separate and remove unencapsulated quantum dots through Sephadex G-50 chromatography column to obtain active targeting liposomes containing quantum dots. Add doxorubicin normal saline solution, 60 ℃ water bath for 20 min. Elute with normal saline and pass thr...

Embodiment 3

[0028] The liposome membrane material modified by RPARPAR was formulated as dilauroylphosphatidylglycerol / cholesterol / methoxypolyethylene glycol-hydrogenated soybean phosphatidylethanolamine / RPARPAR-PEG-DSPE (85:15:2:0.5, mol / mol ). Weigh the above membrane material and dissolve it in chloroform, and remove the organic solvent by rotary evaporation to obtain a uniform lipid membrane. Add an aqueous solution of adenosine-modified CdSe / CdS / ZnS quantum dots for hydration, and shake in a water bath at 60°C for 2 hours to obtain a liposome suspension. In a water bath at 60 °C, use a high-pressure homogenizer to sequentially squeeze liposomes through 400, 200, and 100 nm nuclear pore membranes to reduce their particle size. Then use physiological saline as eluent to separate and remove unencapsulated quantum dots through Sephadex G-50 chromatography column to obtain active targeting liposomes containing quantum dots. Add doxorubicin normal saline solution, 60 ℃ water bath for 20 m...

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Abstract

The invention relates to a method for preparing a dual-function targeting quantum dot lipidosome. The method comprises the following steps: preparing a single quantum dot lipidosome: dissolving phospholipid, cholesterol, methoxy polyethylene glycol-phospholipid complex and NRP-1 ligand polypeptide polyethylene glycol phospholipid complex into chloroform to obtain a uniform lipid membrane, and drying the chloroform by evaporation; dissolving in an ammonium sulfate solution of quantum dots, and oscillating to obtain lipidosome suspension; extruding, and diluting with normal saline to obtain an active targeting lipidosome containing the quantum dots; adding the active targeting lipidosome containing the quantum dots into an adriamycin normal saline solution, and treating in a water bath for 20 minutes at the temperature of 60 DEG C; and diluting with normal saline through a SephadexG-50 gel column, removing free medicaments, and thus obtaining the dual-function targeting quantum dot lipidosome. The lipidosome prepared by the method can be used for marking and treating glioma cells.

Description

technical field [0001] The invention relates to a method for preparing liposomes in the technical field of nanomaterials, in particular to a method for preparing liposomes with bifunctional targeting quantum dots. Background technique [0002] Tumor is a major disease that threatens human health and life, and its incidence has shown an obvious upward trend in recent years. In terms of treatment, surgery cannot completely remove tumor cells and tumor metastatic lymph nodes, resulting in tumor recurrence; chemotherapy drugs are non-selective to tumor tissue after intravenous administration, and have serious systemic toxic and side effects; radiotherapy often causes severe side effects in patients. local skin reactions, blood changes, local mucosal reactions, etc. Since tumor tissue has an EPR (high permeability and retention) effect on nanoparticles with a certain particle size (10-500 nm), that is, nanoparticles in the blood circulation can be passively targeted to tumor tis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/704A61K49/18A61K47/02A61K47/42A61P35/00
Inventor 刘璐闫志强何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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