Method for preparing drug carrier based on magnetic carbon quantum dot/chitosan composite microsphere

Abstract

The invention relates to a method for preparing a drug carrier based on a magnetic carbon quantum dot / chitosan composite microsphere. The method specifically comprises the following steps of: (1) carrying out a coprecipitation reaction on bivalent and trivalent iron salts in an alkaline aqueous solution so as to prepare nano magnetic ferroferric oxide; (2) carrying out microwave radiation reaction on a glucose and polyethylene glycol mixed solution to prepare carbon quantum dots, and forming magnetic carbon quantum dot composite particles through electrostatic adsorption; (3) reacting the chitosan which is dissolved in a mixed solution of sodium methoxide / absolute methanol with nitric oxide in a high-pressure kettle, and forming a chitosan-nitric oxide addition product; and (4) dropwise adding the magnetic carbon quantum dots into the addition product, and forming the magnetic carbon quantum dot / chitosan composite microsphere through electrostatic adsorption. Compared with the prior art, the method is simple, rapid, low in cost, and the prepared product can be developed into the drug carrier which integrates magnetic targeting, fluorescence imaging or tracing, nitric oxide in-situ release and fluorescence detection.

Description

technical field

[0001] The invention belongs to the technical field of material preparation, and in particular relates to a preparation method of a drug carrier based on magnetic carbon quantum dots / chitosan composite microspheres. Background technique

[0002] In recent years, the role of nanomaterials in the fields of biology and medicine has been paid more and more attention. Once they are applied in clinical practice, they will greatly promote the development of traditional medicine. In particular, the clinical application of nano-drug carriers is bound to bring about major changes and innovations in traditional drug delivery methods. However, before the safe and effective use of nanocarriers in clinical practice, the toxicity, biocompatibility, degradability, dispersibility and stability of the carrier must be solved first, and then the drug loading rate of the carrier should be improved, Functions such as targeting, controllability and traceability of drug release. T...

Examples

Embodiment 1

[0028] The preparation process of the drug carrier based on magnetic carbon quantum dots / chitosan composite microspheres can be found in figure 1 , the detailed preparation steps are as follows: Dissolve 0.2mol ferric chloride, 0.1mol ferrous sulfate and 0.3mol thioglycolic acid in 100mL deionized water, stir magnetically and heat up to 60°C, add 1M sodium hydroxide solution dropwise to adjust The pH of the reaction system is 7.5, and a large amount of black ferroferric oxide magnetic nanoparticles are obtained. Then polyethyleneimine is added dropwise to make its mass fraction in the magnetic nanoparticle system 0.1%, to obtain positively charged magnetic particles. After dissolving 1g of glucose with 5mL PEG-200, transfer it to a microwave reactor and react for 1min at a power of 540W to prepare carbon quantum dots. The aqueous solution dispersed with carbon quantum dots is added dropwise to the positive electromagnetic particle system, and the magnetic particles adsorbed o...

Embodiment 2

[0031] Dissolve 0.1mol of ferric chloride, 0.1mol of ferrous sulfate and 0.2mol of thioglycolic acid in 100mL of deionized water, stir magnetically and raise the temperature to 60°C, add 1M sodium hydroxide solution dropwise to adjust the pH of the reaction system to 7.5, and obtain A large number of black ferroferric oxide magnetic nanoparticles. Then polyethyleneimine is added dropwise to make its mass fraction in the magnetic nanoparticle system 0.1%, to obtain positively charged magnetic particles. After dissolving 2g of glucose with 10mL PEG-200, transfer it to a microwave reactor, and react at a power of 540W for 5min to prepare carbon quantum dots. The aqueous solution dispersed with carbon quantum dots is added dropwise to the positive electromagnetic particle system, and the magnetic particles adsorbed on the surface of carbon quantum dots are formed under the action of ultrasound at 20°C, that is, magnetic carbon quantum dots. Chitosan was dissolved in a mixture of ...

Embodiment 3

[0033]Dissolve 0.3 mol of ferric chloride, 0.1 mol of ferrous sulfate and 0.4 mol of thioglycolic acid in 100 mL of deionized water, stir magnetically and raise the temperature to 60°C, add 1M sodium hydroxide solution dropwise to adjust the pH of the reaction system to 7.0, and obtain A large number of black ferroferric oxide magnetic nanoparticles. Then polyethyleneimine is added dropwise to make its mass fraction in the magnetic nanoparticle system 0.1%, to obtain positively charged magnetic particles. After dissolving 3g of glucose in 15mL PEG-200, it was transferred to a microwave reactor and reacted at a power of 540W for 8min to prepare carbon quantum dots. The aqueous solution dispersed with carbon quantum dots is added dropwise to the positive electromagnetic particle system, and the magnetic particles adsorbed on the surface of carbon quantum dots are formed under the action of ultrasound at 20°C, that is, magnetic carbon quantum dots. Chitosan was dissolved in a mi...

technical field

[0001] The invention belongs to the technical field of material preparation, and in particular relates to a preparation method of a drug carrier based on magnetic carbon quantum dots / chitosan composite microspheres. Background technique

[0002] In recent years, the role of nanomaterials in the fields of biology and medicine has been paid more and more attention. Once they are applied in clinical practice, they will greatly promote the development of traditional medicine. In particular, the clinical application of nano-drug carriers is bound to bring about major changes and innovations in traditional drug delivery methods. However, before the safe and effective use of nanocarriers in clinical practice, the toxicity, biocompatibility, degradability, dispersibility and stability of the carrier must be solved first, and then the drug loading rate of the carrier should be improved, Functions such as targeting, controllability and traceability of drug release. T...

Examples

Embodiment 1

[0028] The preparation process of the drug carrier based on magnetic carbon quantum dots / chitosan composite microspheres can be found in figure 1 , the detailed preparation steps are as follows: Dissolve 0.2mol ferric chloride, 0.1mol ferrous sulfate and 0.3mol thioglycolic acid in 100mL deionized water, stir magnetically and heat up to 60°C, add 1M sodium hydroxide solution dropwise to adjust The pH of the reaction system is 7.5, and a large amount of black ferroferric oxide magnetic nanoparticles are obtained. Then polyethyleneimine is added dropwise to make its mass fraction in the magnetic nanoparticle system 0.1%, to obtain positively charged magnetic particles. After dissolving 1g of glucose with 5mL PEG-200, transfer it to a microwave reactor and react for 1min at a power of 540W to prepare carbon quantum dots. The aqueous solution dispersed with carbon quantum dots is added dropwise to the positive electromagnetic particle system, and the magnetic particles adsorbed o...

Embodiment 2

[0031] Dissolve 0.1mol of ferric chloride, 0.1mol of ferrous sulfate and 0.2mol of thioglycolic acid in 100mL of deionized water, stir magnetically and raise the temperature to 60°C, add 1M sodium hydroxide solution dropwise to adjust the pH of the reaction system to 7.5, and obtain A large number of black ferroferric oxide magnetic nanoparticles. Then polyethyleneimine is added dropwise to make its mass fraction in the magnetic nanoparticle system 0.1%, to obtain positively charged magnetic particles. After dissolving 2g of glucose with 10mL PEG-200, transfer it to a microwave reactor, and react at a power of 540W for 5min to prepare carbon quantum dots. The aqueous solution dispersed with carbon quantum dots is added dropwise to the positive electromagnetic particle system, and the magnetic particles adsorbed on the surface of carbon quantum dots are formed under the action of ultrasound at 20°C, that is, magnetic carbon quantum dots. Chitosan was dissolved in a mixture of ...

Embodiment 3

[0033]Dissolve 0.3 mol of ferric chloride, 0.1 mol of ferrous sulfate and 0.4 mol of thioglycolic acid in 100 mL of deionized water, stir magnetically and raise the temperature to 60°C, add 1M sodium hydroxide solution dropwise to adjust the pH of the reaction system to 7.0, and obtain A large number of black ferroferric oxide magnetic nanoparticles. Then polyethyleneimine is added dropwise to make its mass fraction in the magnetic nanoparticle system 0.1%, to obtain positively charged magnetic particles. After dissolving 3g of glucose in 15mL PEG-200, it was transferred to a microwave reactor and reacted at a power of 540W for 8min to prepare carbon quantum dots. The aqueous solution dispersed with carbon quantum dots is added dropwise to the positive electromagnetic particle system, and the magnetic particles adsorbed on the surface of carbon quantum dots are formed under the action of ultrasound at 20°C, that is, magnetic carbon quantum dots. Chitosan was dissolved in a mi...

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