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Gene segment for coding porcine interferon-gamma and application of gene segment

A technology of interferon gamma and gene fragment, which is applied to the gene fragment encoding porcine interferon gamma and its application field, can solve the problems of low codon expression of the original gene and low antiviral activity, and achieves good antiviral effect and expression The effect of high volume and strong market competitiveness

Active Publication Date: 2013-04-03
SOUTH CHINA AGRI UNIV +1
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Problems solved by technology

[0012] However, the above technologies all have the defects of low expression of the original gene codon and low antiviral activity.

Method used

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  • Gene segment for coding porcine interferon-gamma and application of gene segment
  • Gene segment for coding porcine interferon-gamma and application of gene segment
  • Gene segment for coding porcine interferon-gamma and application of gene segment

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Embodiment Construction

[0026] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0027] (1) Genetic modification

[0028] Using DNASTAR (Version 5.0) gene analysis software, refer to the nucleotide sequence of porcine interferon α gene mature peptide (AY331298), porcine interferon β gene mature peptide nucleotide sequence (S41178) and porcine interferon γ gene published on NCBI GenBank Gene mature peptide sequence (EU249804), referring to the codon usage analysis of Pichia pastoris such as Zhao Xiang and the Pichia yeast codon table of TaKaRa company, etc., according to the amino acid sequence of the sequence, the expressed protein does not change, and at the same time according to Based on the partial tropism of codons in the expression system of Pic...

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Abstract

According to the feeding preference of the pichia pastoris expression system codon, a high-expression codon is selected to be redesigned and synthetized into a novel porcine interferon-gamma mature peptide nucleotide sequence. A recombinant yeast expression plasmid pPICZalphaC-IFN-gamma is successfully constructed, so that the correct expression of the porcine interferon-gamma is achieved in the pichia pastoris, the porcine interferon-gamma is expressed more easily in the pichia pastoris, the expression quantity is higher, and the foundation is laid from the large-scale production of gene engineering.

Description

[0001] This application is a divisional application of the patent application with the application date of March 26, 2010, the application number: 201010139485.4, and the title of the invention is "gene fragment encoding porcine alpha interferon and its application". technical field [0002] The invention relates to a gene segment encoding porcine gamma interferon and its application. Background technique [0003] In recent years, porcine viral infectious diseases have become increasingly rampant, which has seriously threatened the sustainable development of animal husbandry in my country. Immunosuppressive diseases and rapid mutation of viral antigens often lead to vaccine immunization failure; coupled with the continuous emergence of new viral diseases, there is no successful vaccination prevention yet. Vaccines, on the other hand, only prevent disease, and treatment also depends on the use of antibiotics. However, the emergence of some drug-resistant strains poses a grea...

Claims

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Application Information

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IPC IPC(8): C12N15/23C12N15/81C07K14/57A61K38/21A61P31/12A61P37/04C12R1/84
Inventor 罗满林陈瑞爱刘健张欣唐明森
Owner SOUTH CHINA AGRI UNIV
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