FGFR3 gene mutation detection specific primer and liquid chip

Abstract

The invention discloses an FGFR3 gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.22 and SEQ ID NO.23, SEQ ID NO.24 and SEQ ID NO.25, SEQ ID NO.26 and SEQ ID NO.27, SEQ ID NO.28 and SEQ ID NO.29, SEQ ID NO.30 and SEQ ID NO.31, SEQ ID NO.32 and SEQ ID NO.33, SEQ ID NO.34 and SEQ ID NO.35, SEQ ID NO.36 and more than one of SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39 and SEQ ID NO.40, and / or SEQ ID NO.41 and SEQ ID NO.42. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, in particular to a specific primer for FGFR3 gene mutation detection and a liquid phase chip. Background technique [0002] Fibroblast growth factors (fibroblast growth factors, FGFs) are a family of polypeptides with similar structures, and act through the transmembrane high-affinity fibroblast growth factor receptors (fibroblast growth factor receptors, FGFRs). Fibroblast growth factor receptor 3 (Fibroblast growth factor receptors 3, FGFR3) is a tyrosine kinase receptor, which plays an important role in the occurrence of cell mitosis, promotion of angiogenesis, wound healing and tumor formation. FGFR3 is a transmembrane protein. When the extracellular part of the receptor binds to the ligand FGFs, the receptor forms a homologous or heterodimer, activates the tyrosine kinase in the intracellular part, and causes a series of cascades in the cell reaction, ...

AI Technical Summary

Problems solved by technology

The PCR-RFLP method is based on the change of the restriction endonuclease recognition site caused by gene mutation, such as the loss of the site or the generation of a new site, a specific fragment is amplified by PCR, and then cut and amplified with a restriction endonuclease. Amplify the product and observe the size of the fragment by electrophoresis. This method is used to detect gene mutations with altered restriction sites, and can directly determine the genotype. However, this method cannot be used for the detection of gene mutations without new restriction sites.

Again, the above methods all have limitations in detection throughput, and can only detect one mutation type at a time, which cannot meet the needs of practical applications.

Images